Intraspecific variation and geographic study of Lonicera insularis (Caprifoliaceae) based on chloroplast DNA sequences

Abstract

Lonicera insularis Nakai (Caprifoliaceae) is Korean endemic plant that lives along the shore of Ulleungdo and Dokdo. The aim of this study is to construct a phylogenetic relationship within six species (L.maackii, L.chrysantha, L.subsessilis, L. harai, L. morrowii) of genus Lonicera L. and Wigela subsessilis as outgroup and intraspecific variation of L. insularis using chloroplast DNA five regions sequences. Sequence analysis revealed that both L. insularis and L. morrowii showed complete homologies in the intergenic regions of trnL-trnF, trnS-trnG, psbM-trnD and matK coding region. However, sequence in the petN-psbM intergenic region showed a single nucleotide difference between both species, thus we designated them as CP01 and CP02. The plants having CP01 are prevalent in the Ulleungdo and Dokdo, while L. insularis and L. morrowii from Ulleungdo and of Dokdo, and Japan have CP02. This confirmed the existence of two cp DNA lineages with different geographical distributions. We can infer the allopatric speciation by geographical barrier. The result will provide the important basal data to study speciation and specie evolution of ocean islands such as Ulleungdo and Dokdo.

A report of the second chloroplast genome sequence in Veronica nakaiana (Plantaginaceae), an endemic species in Korea

“…Variable haplotypes in cpDNA regions were recognized in Campanula takesimana Nakai (rps16-trnK, trnQ-rps16, psbD-trnT, and psbM-trnD) (Cheong et al, 2020) and Rubus takesimensis Nakai (trnL-trnF) (Lee et al, 2017). In contrast, no haplotype variations were reported in the selected cp DNA regions for Lonicera insularis Nakai (trnL-trnF, trnS-trnG, psbM-trnD, and matK) (Jeong et al, 2014) and Fagus multinervis Nakai (trnH-psbA) (Oh, 2015). Analysis of sequence variation between two cp genomes in V. nakaiana in this study is a useful resource to understand the evolution and diversification of this species.…”

“…Many studies on endemic species of Ulleungdo Island have been conducted to understand the evolution of genetic diversification of the species on Ulleungdo Island of volcanic origin. However, all of these studies are based on sequence data from a few DNA regions (e.g., Jeong et al, 2014;Oh, 2015;Lee et al, 2017;Cheong et al, 2020), often resulting in the lack of molecular variations within the species. Therefore, studies based on the whole chloroplast (cp) genome are needed in future population genetic studies of endemic species (Park et al, 2020).…”

Ecophysiology of seed dormancy and germination in four Lonicera (Caprifoliaceae) species native to Korea

“…There are 17 Lonicera species that are native to Korea (Lee 2003). Especially, L. insularis Nakai and L. subsessilis Rehder are Korean endemic plants that are distributed along the shore of Ulleungdo and from Pyeongannam-do to Jeollanam-do in Korea, respectively (Jeong et al 2014;KNA 2019). To exploit these plant resources, investigating germination characteristics and seed dormancy type is needed.…”

Morphological variation and aspects of the geographic distribution of Orobanche coerulescens Stephan ex Willd. (Orobanchaceae) on Ulleung-do and Dok-do Islands

“…)은 열당 과(Orobanchaceae) 초종용속(Orobanche L.)에 속하고, 초종 용속은 열당과에서 170여 종의 기생식물(parasitic plant)을 포함하는 속으로 주로 북반구에 분포한다 (Young et al, 1999 (An and Hong, 2003;Hyun et al, 2003;Hong, 2007;Sun et al, 2014). 초종용은 주로 중앙유럽 (오스트리아, 독일 등)에서부터 중앙아시아(카자흐스탄, 몽골 등)를 거쳐 한국, 러시아, 중국, 일본, 대만 등지에 넓 게 분포한다 (Hsiao, 1978;Zhang and Tzvelev, 1998;Novopokrovskii, 2000;Al-Shehbaz et al, 2006;Hong, 2007;Piwowarczyk and Przemyski, 2009;Piwowarczyk, 2012 (Pfosser et al, 2002(Pfosser et al, , 2005Lee and Pak, 2010;Jeong et al, 2014 (Young et al, 1999;Gerald, 2004;Manen et al, 2004, Gevezova et al 2012 (Hsiao, 1978;Zhang and Tzvelev, 1998;Novopokrovskii, 2000;Al-Shehbaz et al, 2006;Hong, 2007 (Wolfe and dePamphilis, 1997;Young et al, 1999;Manen et al, 2004;Schneeweiss et al, 2004;Park et al, 2008 (Hsiao, 1978;Zhang and Tzvelev, 1998;Novopokrovskii, 2000;Al-Shehbaz et al, 2006;Hong, 2007;Piwowarczyk and Przemyski, 2009;Piwowarczyk, 2012…”