Intraspecific variability of the facultative meiotic parthenogenetic root-knot nematode (Meloidogyne graminicola) from rice fields in Vietnam

Bellafiore, Stéphane; Jougla, Claire; Chapuis, Élodie; Besnard, Guillaume; Suong, Malyna; Vu, Phong Nguyen; Waele, Dirk De; Gantet, Pascal; Thi, Xuyen Ngo

doi:10.1016/j.crvi.2015.04.002

Cited by 37 publications

(52 citation statements)

References 27 publications

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“…Moreover, one would expect within the MF species a large pathogenic and genetic variability like in other meiotic parthenogenetic Meloidogyne spp. such as M. chitwoodi , M. fallax , M. hapla , M. exigua and M. graminicola (Bellafiore et al., ; Janssen, Van Norel, Verkerk‐Bakker, & Janssen, ; Muniz et al., ; Van der Beek, Poleij, Zijlstra, Janssen, & Janssen, ). Thus, it is important to evaluate resistance against different MF isolates and RKN species to determine specificity of the Mf gene in P. kansuensis .…”

Section: Discussionmentioning

confidence: 99%

Inheritance of resistance to the peach root‐knot nematode (Meloidogyne floridensis) in interspecific crosses between peach (Prunus persica) and its wild relative (Prunus kansuensis)

et al. 2018

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The peach root‐knot nematode, Meloidogyne floridensis (MF), infects majority of available nematode‐resistant peach rootstocks which are mostly derived from peach (Prunus persica) and Chinese wild peach (P. davidiana). Interspecific hybridization of peach with its wild relative, Kansu peach (P. kansuensis), offers potential for broadening the resistance spectrum in standard peach rootstocks. We investigated the inheritance of resistance to MF in segregating populations of peach (‘Okinawa’ or ‘Flordaguard’) × P. kansuensis. A total of 379 individuals from 13 F2 and BC1F1 families were challenged with a pathogenic MF isolate “MFGnv14” and were classified as resistant (R) or susceptible (S) based on root galling intensity. Segregation analyses in F2 progeny revealed the involvement of a major locus with a dominant or recessive allele determining resistance in progeny segregating 3R:1S and 1R:3S, respectively. Testcrosses with a homozygous‐susceptible peach genotype (‘Flordaguard’ or ‘UFSharp’) confirmed P. kansuensis as a source of new resistance and the heterozygous allelic status of P. kansuensis at the locus conferring resistance to MF. We propose a single‐locus dominant/recessive model for the inheritance of resistance.

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Section: Discussionmentioning

confidence: 99%

Inheritance of resistance to the peach root‐knot nematode (Meloidogyne floridensis) in interspecific crosses between peach (Prunus persica) and its wild relative (Prunus kansuensis)

et al. 2018

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“…In spite of morphological and morphometrical differences, the ITS marker-based molecular analysis revealed that all the nematodes belonged to one species-M. graminicola. It is possible that these morphometrical differences may be because of geographical intraspecific variability not associated with a molecular marker, presenting another example of either phenotypic plasticity exhibited by nematodes (Viney and Diaz, 2012;Nijhout, 2015;de Oliveira et al, 2017), or the presence of races within M. graminicola (Pokharel et al, 2010;Bellafiore et al, 2015). In a previous study, 10 different geographical isolates of M. graminicola showed substantial variation in morphometric measurements, yet the ITS sequences of all 10 isolates identified them as M. graminicola (Pokharel et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study, 10 different geographical isolates of M. graminicola showed substantial variation in morphometric measurements, yet the ITS sequences of all 10 isolates identified them as M. graminicola (Pokharel et al, 2010). Several studies (Jepson, 1983;Pokharael et al, 2007Pokharael et al, , 2010Bellafiore et al, 2015) used morphometrical characters to reveal FIG. 4.…”

Section: Discussionmentioning

confidence: 99%

“…Identification and diagnosis of M. graminicola has always been a challenge (Hunt and Handoo, 2009), and many markers based on nuclear or mitochondrial DNA have been used including the ITS region (Pokharel et al, 2007;Besnard et al, 2014;Bellafiore et al, 2015). It has been suggested that mitochondrial phylogenetic markers including variable number tandem repeats could help resolve the population diversity better than nuclear markers (Lunt et al, 1998;Besnard et al, 2014;Sun et al, 2014;Humphreys-Pereira and Elling, 2015).…”

Section: Discussionmentioning

confidence: 99%

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Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of Meloidogyne graminicola

Salalia

Walia

Somvanshi

et al. 2017

Journal of Nematology

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Fourteen populations of Meloidogyne graminicola were collected from different agroecological regions of India. Morphological and morphometrical comparisons were made for various nematode life stages. Three populations (Hisar, New Delhi, and Samastipur) were different from typical M. graminicola on the basis of the length of eggs; J2 length, a-value, hyaline tail portion; male length, distance up to excretory pore, spicule and gubernaculum lengths; female length and width, stylet length, distance up to excretory pore, EPST (distance of excretory pore from anterior end / stylet length [females]) ratio, and vulval length. Morphological and morphometrical comparison with closely related species M. graminis, M. oryzae, M. salasi, M. triticoryzae, and M. lini clustered these populations into two groups: Anand, Bhubaneswar, Hyderabad, Jammu, Jorhat, Kalyani, Kanpur, Ludhiana, Mandya, Palampur, Vellayani grouped with M. graminicola, M. triticoryzae and M. salasi; whereas, Hisar, New Delhi, Samastipur grouped with M. oryzae and M. graminis. Molecular phylogenetic analysis using internal transcribed spacer (ITS) suggested that in spite of morphological differences, these populations belonged to M. graminicola.

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“…DNA of all purified populations was extracted according to Randig et al (2002) and tested with specific SCAR markers developed by Bellafiore et al (2015) and Htay et al (2016). PCR studies targeting the ITS1-5.8S-ITS2 rRNA region were carried out using the primer set developed by Schmitz et al (1998), i.e., forward primer 5 -TTGATTACGTCCCTGCCCTTT-3 and reverse primer 5 -TCCTCCGCTAAATGATATG-3 , and amplification conditions according to Subbotin et al (2000).…”

Section: Populationsmentioning

confidence: 99%

Characterisation of a Meloidogyne species complex parasitising rice in southern Brazil

Negretti

Gomes

Mattos

et al. 2017

Nematol

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Root-knot nematodes (RKN) are important plant pathogens affecting rice in South-East Asia and southern Brazil in irrigated rice fields. In order to investigate the specific diversity of RKN associated with irrigated rice in southern Brazil, Meloidogyne spp. from Rio Grande do Sul (RS) and Santa Catarina (SC) States were characterised biochemically by esterase (Est) and malate dehydrogenase (Mdh) phenotypes. Fifty-six Meloidogyne spp. populations were detected in 48% of rice samples, and a total of fiveesterase phenotypes were identified, four of which presented as drawn-out bands in different positions. In RS State, M. graminicola (Est VS1), Meloidogyne sp. 2 (Est R2) and Meloidogyne sp. 3 (Est R3) were identified, which corresponded to ca 80, 40 and 10% of samples, respectively. In SC State, M. graminicola, M. javanica (Est J3), Meloidogyne sp. 1 (Est R1), Meloidogyne sp. 2 and Meloidogyne sp.3 accounted for ca 93.75, 12.50, 62.50, 12.25 and 6.25% of samples, respectively. The esterase phenotypes R1, R2 and R3 are new, never having been detected on rice before. Meloidogyne javanica showed a N1 Mdh phenotype (Rm: 1.0), while four other populations exhibited a N1a (Rm: 1.4) phenotype. All populations were tested with two SCAR markers specific to M. graminicola, which confirmed that, but no specificity was obtained with both markers in relation to the atypical populations analysed. Sequencing and phylogenetic analyses of internal transcribed spacer-rRNA (ITS) were performed to infer the phylogenetic relationship of these atypical Meloidogyne spp. populations. Meloidogyne sp. 1 grouped with the mitotic parthenogenetic species, while the two others (Meloidogyne sp. 2 and sp. 3) clustered with M. graminicola and other meiotic parthenogenetic species. Taken together, these data highlight the unprecedented specific diversity of RKN associated with irrigated rice in southern Brazil. Further morphological and phylogenetic studies involvingthese atypical isolates will be carried out to identify this complex of species

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Intraspecific variability of the facultative meiotic parthenogenetic root-knot nematode (Meloidogyne graminicola) from rice fields in Vietnam

Cited by 37 publications

References 27 publications

Inheritance of resistance to the peach root‐knot nematode (Meloidogyne floridensis) in interspecific crosses between peach (Prunus persica) and its wild relative (Prunus kansuensis)

Inheritance of resistance to the peach root‐knot nematode (Meloidogyne floridensis) in interspecific crosses between peach (Prunus persica) and its wild relative (Prunus kansuensis)

Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of Meloidogyne graminicola

Characterisation of a Meloidogyne species complex parasitising rice in southern Brazil

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