Intraspecific classification of Ficus deltoidea Jack subsp. deltoidea (Moraceae) in Peninsular Malaysia based on morphological and molecular variations

Tnah, Lee Hong; Lee, Soon Leong; Tan, Ai Lee; Lee, Chai Ting; Ng, Kevin Kit Siong; Ng, Chin Hong

doi:10.1016/j.bse.2016.06.001

Cited by 4 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ten chloroplast (cpDNA) intergenic regions, including ndhF-rpl32, psbA-trnH, psbB-psbF, psbC-trnS, psbJ-petA, trnD-trnT, trnF-trnV, trnL-trnF, trnQ-rps16, and trnS-trnG [33][34][35], were tested for amplification and sequence variation. Some of these markers are recognised as informative from earlier phylogenetic and phylogeographic research of Ficus plants [35][36][37][38]. To test the ten candidate cpDNA markers, one or two individuals were randomly selected from each sampled population for PCR amplification using universal primer pairs under the conditions described by Shaw et al [33,34] and Vieira et al [35].…”

Section: Dna Extraction and Chloroplast Dnamentioning

confidence: 99%

Homogenized Phylogeographic Structure across the Indo-Burma Ranges of a Large Monoecious Fig, Ficus altissima Blume

2021

View full text Add to dashboard Cite

As well as bountiful natural resources, the Indo-Burma biodiversity hotspot features high rates of habitat destruction and fragmentation due to increasing human activity; however, most of the Indo-Burma species are poorly studied. The exploration of plants closely associated with human activity will further assist us to understand our influence in the context of the ongoing extinction events in the Anthropocene. This study, based on widely and intensively sampled F. altissima across Indo-Burma and the adjacent south China ranges, using both the chloroplast psbA-trnH spacer and sixteen newly developed nuclear microsatellite markers (nSSRs), aims to explore its spatial genetic structure. The results indicated low chloroplast haplotype diversity and a moderate level of nuclear genetic diversity. Although limited seed flow was revealed by psbA-trnH, no discernible phylogeographic structure was shown due to the low resolution of cpDNA markers and dominance of an ancestral haplotype. From the nSSRs data set, phylogeographic structure was homogenized, most likely due to extensive pollen flow mediated by pollinating fig wasps. Additionally, human cultivation and human-mediated transplanting further confounded the analyses of population structure. No geographic barriers are evident across the large study range, with F. altissima constituting a single population, and extensive human cultivation is likely to have had beneficial consequences for protecting the genetic diversity of F. altissima.

show abstract

Section: Dna Extraction and Chloroplast Dnamentioning

confidence: 99%

Homogenized Phylogeographic Structure across the Indo-Burma Ranges of a Large Monoecious Fig, Ficus altissima Blume

2021

View full text Add to dashboard Cite

show abstract

“…Therefore, study variation in intraspecific taxa requires a combination of several characters. Molecular characters can be used to reveal genetic variation and phylogenetic relationships because they are more varied (Nazar and Mahmood 2011;Tnah et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data

et al. 2020

View full text Add to dashboard Cite

Abstract.Ningrum WDA, Atmaja MB, Daryono BS, Purnomo. 2020. Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data. Biodiversitas 21: 5778-5785. Begonia longifolia Blume is a member of the Begoniaceae family which has the widest distribution in Asia including Indonesia. Diversity studies on B.longifolia from Indonesia were mainly based on morphological characters. The genetic diversity analysis of this pantropical species in Indonesia has never been reported yet. This research aimed to study genetic variability of B. longifolia in Indonesia based on nuclear DNA internal transcribed spacer (ITS) sequence data. Twelve living specimens were from Sumatra (Lampung), Java (Sukabumi, Purwokerto), Bali (Buleleng, Gianyar, Jembrana, Tabanan), and Lombok. The 831-839 length sequence was aligned using Mesquite software. Reconstruction of the phylogenetic tree was done by MEGA7 software using Neighbor-Joining method with Kimura-2-Parameter. The 827 fixed sites showed a genetic distance ranged from 0 to 1.6%. Among the 827 sites compared, 802 sites were invariable, 19 sites were variable (11 singleton variable sites and 8 parsimony informative sites) and 6 sites were gaps. The phylogenetic tree showed 1 clade and 6 sub-clades. Specimens from Sumatra and Java formed a single sub-clade, separate with five sub-clades from Bali. Specimen from Lombok belong to one of the sub-clades of the Bali specimens. All specimens can be divided into 2 groups: Sumatra-Java and Bali-Lombok group.

show abstract

Ficus deltoidea: Potential inhibitor of pro-inflammatory mediators in lipopolysaccharide-induced activation of microglial cells

Zolkiffly

Stanslas

Hamid

et al. 2021

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Intraspecific classification of Ficus deltoidea Jack subsp. deltoidea (Moraceae) in Peninsular Malaysia based on morphological and molecular variations

Cited by 4 publications

References 29 publications

Homogenized Phylogeographic Structure across the Indo-Burma Ranges of a Large Monoecious Fig, Ficus altissima Blume

Homogenized Phylogeographic Structure across the Indo-Burma Ranges of a Large Monoecious Fig, Ficus altissima Blume

Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data

Ficus deltoidea: Potential inhibitor of pro-inflammatory mediators in lipopolysaccharide-induced activation of microglial cells

Contact Info

Product

Resources

About