Intrarectal Administration of Arsphenamin

Abstract

reliable, however, as repeated trials with other strains of B. botulinus have always been unsuccessful. The technic of making cultures from the spleen does not differ in any way from that of making routine cultures from tissues at the necropsy table. Portions of the spleen are removed through a seared surface, and inoculated into glucose-agar and glucose-broth cul¬ ture tubes. We have not found it necessary to grind up the tissue before inoculation, as B. botulinus, if present, will soon spread out from the small pieces of tissue. If cultures are obtained, the bacilli are iso¬ lated and identified in the manner already described.