Intrapulmonary percussive ventilation improves lung function in cystic fibrosis patients chronically colonized with Pseudomonas aeruginosa: a pilot cross-over study

Dingemans, Jozef; Eyns, H.; Willekens, Julie; Monsieurs, Pieter; Cornélis, Pierre; Malfroot, Anne; Crabbé, Aurélie

doi:10.1007/s10096-018-3232-8

Cited by 12 publications

(11 citation statements)

References 42 publications

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“…A). However, under biofilm conditions, several biofilm marker genes, such as pelA , pslG , glpD , anr and dnr (Goodman et al , ; Son et al , ; Crabbe et al , ; ; Bielecki et al , ; Dingemans et al , ; ) as well as genes contributing to motility ( fliC, pilY1 ) were >2‐fold down‐regulated in biofilms by Δ sagS strain relative to Δ sagS::sagS (Fig. B) .…”

Section: Resultsmentioning

confidence: 97%

Controlling chronic Pseudomonas aeruginosa infections by strategically interfering with the sensory function of SagS

Dingemans

Al-Feghali

Lau

et al. 2019

Molecular Microbiology

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The hybrid sensor SagS plays a central role in the formation of Pseudomonas aeruginosa biofilms, by enabling the switch from the planktonic to the biofilm mode of growth and by facilitating the transition of biofilm cells to a highly tolerant state. In this study, we examined the importance of the SagS key amino acid residues associated with biofilm formation (L154) and antibiotic tolerance (D105) in P. aeruginosa virulence. Recombinant P. aeruginosa ΔsagS and ΔsagS chromosomally expressing wildtype sagS, or its two variants D105A and L154A, were tested for their potential to form biofilms and cause virulence in plants and mouse models of acute and chronic pneumonia. Although mutation of sagS did not alter P. aeruginosa virulence during acute infections, a significant difference in pathogenicity of sagS mutants was observed during chronic infections, with the L154A variant showing reduced bacterial loads in the chronic pneumonia model, while interference with the D105 residue enhanced the susceptibility of P. aeruginosa biofilms during tobramycin treatment. Our findings suggest that interference with the biofilm or tolerance regulatory circuits of SagS affects P. aeruginosa pathogenicity in chronic but not acute infections, and reveal SagS to be a promising new target to treat P. aeruginosa biofilm infections.

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Section: Resultsmentioning

confidence: 97%

Controlling chronic Pseudomonas aeruginosa infections by strategically interfering with the sensory function of SagS

Dingemans

Al-Feghali

Lau

et al. 2019

Molecular Microbiology

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“…Additionally, some studies may use markers, such as sputum weight, which may be unfeasible in a pediatric population. Rigorous assessments of these methodologies may be increasingly important as new devices, including portable devices lacking clinical evidence, are proposed for patient use.…”

Section: Discussionmentioning

confidence: 99%

The effects of high‐frequency chest compression on end‐tidal CO₂

Weiner

Forno

Weiner

2019

Pediatric Pulmonology

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Introduction High‐frequency chest compression (HFCC) is used for airway clearance, but may have other effects. We sought to determine if HFCC provides augmented ventilation. Methods During treatment, capnometry was measured with the HFCC vest set to 6‐20 Hz. End‐tidal CO2 (etCO2) was compared using generalized estimating equations. Results Twenty‐four measurements were obtained from 15 subjects with mean age 15.2 ± 2.5 years and forced expiratory volume in one second (FEV1) % predicted 70 ± 23. EtCO2 decreased with HFCC at 6 Hz when compared with baseline (P < .001), with small changes with increasing oscillation frequency. Change in etCO2 was not predicted by FEV1, body mass index, age, or sex. Conclusions While HFCC has been shown to be a suitable method of airway clearance, investigators have failed to demonstrate differences between techniques. Assessment of these methodologies will become important as new airway clearance devices are proposed. Other outcome measures (besides FEV1) may be needed to assess effects of airway clearance, and we propose that physiologic measures might be one such measure which deserves further exploration.

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“…No significant changes were found over one year in either the PEP group or the OPEP group [46]. A pilot study by Dingemans et al, studied the influence of shear stress induced by IPV on the bacterial load and gene expression of P. aeruginosa in adults with CF [90]. They concluded that IPV at high frequency could potentially alter the behavior of P. aeruginosa, but this should be verified in a larger group of patients.…”

Section: Othermentioning

confidence: 99%

Outcome measures for airway clearance techniques in children with chronic obstructive lung diseases: a systematic review

et al. 2020

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Background: Airway clearance techniques (ACTs) are an important aspect of the treatment of children with chronic obstructive lung diseases. Unfortunately, a sound evidence base is lacking and airway clearance strategies are largely based on clinical expertise. One of the reasons for the limited evidence is the lack of appropriate outcome measures specifically related to the effectiveness of ACTs. This review discusses all outcome measures applied in previous research in the pediatric population to provide a baseline for future studies. Data sources: A systematic literature search was performed in PubMed, Web of Science and EMBASE databases. Search terms included chronic obstructive lung diseases and ACTs. Study selection: Studies were independently selected by the investigators according to the eligibility criteria. After screening, 49 articles remained for further analysis. Results and conclusions: Data are summarized according to the type of outcome measure. 48 (98%) studies performed pulmonary function tests, 19 (39%) assessed expectorated sputum, 10 (20%) parameters related to disease exacerbation, 8 (16%) oxygenation, 8 (16%) patient-reported outcomes, 5 (10%) exercise capacity and 5 (10%) applied imaging techniques. The synthesis of results showed a high discrepancy between studies due to differences in study design, population and the application of techniques. Since no 'gold standard' method could be identified, a combination of different outcome measures is recommended to gain a better understanding and to identify the potential effects of ACTs. An overview of important considerations has been provided to assist researchers in their choice of outcomes in future studies.

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Intrapulmonary percussive ventilation improves lung function in cystic fibrosis patients chronically colonized with Pseudomonas aeruginosa: a pilot cross-over study

Abstract: The trail was retrospectively registered at the ISRCTN registry on 6 June 2013, under trial registration number ISRCTN75391385.

Cited by 12 publications

References 42 publications

Controlling chronic Pseudomonas aeruginosa infections by strategically interfering with the sensory function of SagS

Controlling chronic Pseudomonas aeruginosa infections by strategically interfering with the sensory function of SagS

The effects of high‐frequency chest compression on end‐tidal CO₂

Outcome measures for airway clearance techniques in children with chronic obstructive lung diseases: a systematic review

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Intrapulmonary percussive ventilation improves lung function in cystic fibrosis patients chronically colonized with Pseudomonas aeruginosa: a pilot cross-over study

Abstract: The trail was retrospectively registered at the ISRCTN registry on 6 June 2013, under trial registration number ISRCTN75391385.

Cited by 12 publications

References 42 publications

Controlling chronic Pseudomonas aeruginosa infections by strategically interfering with the sensory function of SagS

Controlling chronic Pseudomonas aeruginosa infections by strategically interfering with the sensory function of SagS

The effects of high‐frequency chest compression on end‐tidal CO2

Outcome measures for airway clearance techniques in children with chronic obstructive lung diseases: a systematic review

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The effects of high‐frequency chest compression on end‐tidal CO₂