Intraperitoneal Phototherapy Suppresses Inflammatory Reactions in a Surgical Model of Peritonitis

Hara, Takao; Etoh, Tsuyoshi; Itai, Y; Kono, Yohei; Shiroshita, Hidefumi; Shiraishi, Norio; Ito, Masafumi

doi:10.1016/j.jss.2020.03.020

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…The impact of visible or non-infrared light on vitamin D 3 biosynthesis should be further explored using full-spectrum light with UV cut-off filters. In addition to the clinical impact of phototherapy in terms of the elevated vitamin D 3 in NASH patients [35], we and our collaborator have demonstrated the therapeutic potential of phototherapy in experimental animal models of colitis [39] peritonitis [40], and food allergies [41]. Although the mode of action of phototherapy with full-spectrum light has not been fully elucidated, it may regulate proinflammatory cytokine signaling and oxidative stress and maintain optimal levels of vitamin D 3 and healthy microbiota composition.…”

Section: Phototherapy For Active and Healthy Aging: History And Current Applicationsmentioning

confidence: 98%

Sunlight Exposure and Phototherapy: Perspectives for Healthy Aging in an Era of COVID-19

Nakano

Chiang

Chen

et al. 2021

IJERPH

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Most humans depend on sunlight exposure to satisfy their requirements for vitamin D3. However, the destruction of the ozone layer in the past few decades has increased the risk of skin aging and wrinkling caused by excessive exposure to ultraviolet (UV) radiation, which may also promote the risk of skin cancer development. The promotion of public health recommendations to avoid sunlight exposure would reduce the risk of skin cancer, but it would also enhance the risk of vitamin D3 insufficiency/deficiency, which may cause disease development and progression. In addition, the ongoing global COVID-19 pandemic may further reduce sunlight exposure due to stay-at-home policies, resulting in difficulty in active and healthy aging. In this review article, we performed a literature search in PubMed and provided an overview of basic and clinical data regarding the impact of sunlight exposure and vitamin D3 on public health. We also discuss the potential mechanisms and clinical value of phototherapy with a full-spectrum light (notably blue, red, and near-infrared light) as an alternative to sunlight exposure, which may contribute to combating COVID-19 and promoting active and healthy aging in current aged/superaged societies.

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Section: Phototherapy For Active and Healthy Aging: History And Current Applicationsmentioning

confidence: 98%

Sunlight Exposure and Phototherapy: Perspectives for Healthy Aging in an Era of COVID-19

Nakano

Chiang

Chen

et al. 2021

IJERPH

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“…The disruption of the blood-brain barrier (BBB) by systemic inflammation and by brain infiltration of immune cells/inflammatory mediators increases neuroinflammation and exacerbates clinical symptoms and neuronal death in patients with neurodegenerative diseases [17][18][19][20]. Because the BBB contains brain vascular endothelial cells as a main element [21] and because peripheral administration of LPS causes systemic inflammation [22][23][24][25], LPS-treated endothelial cells are widely used to investigate the mechanisms underlying the BBB dysfunction associated with systemic inflammation [26][27][28]. Activation of endothelial cells is triggered by high levels of LPS via a receptor complex of TLR4, CD14 and MD2, resulting in endothelial cell apoptosis and potentially severe vascular collapse in sepsis [26].…”

Section: Introductionmentioning

confidence: 99%

A novel co-culture model for investigation of the effects of LPS-induced macrophage-derived cytokines on brain endothelial cells

Yang

Bhalla

et al. 2023

PLoS ONE

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In order to study effects of macrophage-derived inflammatory mediators associated with systemic inflammation on brain endothelial cells, we have established a co-culture system consisting of bEnd.3 cells and LPS-activated Raw 264.7 cells and performed its cytokine profiling. The cytokine profile of the co-culture model was compared to that of mice treated with intraperitoneal LPS injection. We found that, among cytokines profiled, eight cytokines/chemokines were similarly upregulated in both in vivo mouse and in vitro co-culture model. In contrast to the co-culture model, the cytokine profile of a common mono-culture system consisting of only LPS-activated bEnd.3 cells had little similarity to that of the in vivo mouse model. These results indicate that the co-culture of bEnd.3 cells with LPS-activated Raw 264.7 cells is a better model than the common mono-culture of LPS-activated bEnd.3 cells to investigate the molecular mechanism in endothelial cells, by which systemic inflammation induces neuroinflammation. Moreover, fibrinogen adherence both to bEnd.3 cells in the co-culture and to brain blood vessels in a LPS-treated animal model of Alzheimer’s disease increased. To the best of our knowledge, this is the first to utilize bEnd.3 cells co-cultured with LPS-activated Raw 264.7 cells as an in vitro model to investigate the consequence of macrophage-derived inflammatory mediators on brain endothelial cells.

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“…KA inhibits a series of index in LPS-induced peritonitis in mice, such as TNF-α, IL-33, and IL-1β [14]. (3) Certain physical stimuli also suppress the inflammatory reaction of LPS-induced peritonitis in mice, such as artificial sunlight [16] and low-level laser therapy [17]. Very recently, DUSP11 (dual-specificity phosphatase 11, DUSP11) is reported to inhibit LPS-induced peritonitis in mice.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics Changes in the Peritoneum of Mice with Lipopolysaccharide-Induced Peritonitis

Liu

Zhang

Sun

et al. 2021

IJMS

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Peritonitis caused by LPS is a severe clinical challenge, which causes organ damage and death. However, the mechanism of LPS-induced peritonitis has not been fully revealed yet. Here, we investigated the transcriptome profile of the peritoneal tissue of LPS-induced peritonitis in mice. A model of LPS-induced peritonitis in mice was established (LPS 10 mg/kg, i.p.), and the influence of TAK 242 (TLR4 inhibitor) on the level of inflammatory cytokines in mouse peritoneal lavage fluid was investigated by using an ELISA test. Next, the peritoneal tissues of the three groups of mice (Control, LPS, and LPS+TAK 242) (n = 6) were isolated and subjected to RNA-seq, followed by a series of bioinformatics analyses, including differentially expressed genes (DEGs), enrichment pathway, protein-protein interaction, and transcription factor pathway. Then, qPCR verified-hub genes that may interact with TAK 242 were obtained. Subsequently, the three-dimensional structure of hub proteins was obtained by using homology modeling and molecular dynamics optimization (300 ns). Finally, the virtual docking between TAK 242 and hub proteins was analyzed. Our results showed that TAK 242 significantly inhibited the production of inflammatory cytokines in the peritoneal lavage fluid of mice with peritonitis, including IL-6, IFN-γ, IL-1β, NO, and TNF-α. Compared with the Control group, LPS treatment induced 4201 DEGs (2442 down-regulated DEGs and 1759 up-regulated DEGs). Compared with the LPS group, 30 DEGs were affected by TAK 242 (8 down-regulated DEGs and 22 up-regulated DEGs). A total of 10 TAK 242-triggered hub genes were obtained, and the possible docking modes between TAK 242 and hub proteins were acquired. Overall, our data demonstrated that a large number of DEGs were affected in LPS-triggered peritonitis mice. Moreover, the TLR4 inhibitor TAK 242 is capable of suppressing the inflammatory response of LPS-induced peritonitis. Our work provides clues for understanding the pathogenesis of LPS-induced peritonitis in mice.

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Intraperitoneal Phototherapy Suppresses Inflammatory Reactions in a Surgical Model of Peritonitis

Cited by 4 publications

References 42 publications

Sunlight Exposure and Phototherapy: Perspectives for Healthy Aging in an Era of COVID-19

Sunlight Exposure and Phototherapy: Perspectives for Healthy Aging in an Era of COVID-19

A novel co-culture model for investigation of the effects of LPS-induced macrophage-derived cytokines on brain endothelial cells

Transcriptomics Changes in the Peritoneum of Mice with Lipopolysaccharide-Induced Peritonitis

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