Intraperitoneal Oxidative Stress in Rabbits with Papillomavirus-Associated Head and Neck Cancer Induces Tumoricidal Immune Response That Is Adoptively Transferable

Rossmann, Annette; Mandic, Robert; Heinis, Jochen; Höffken, H.; Küssner, Oliver; Kinscherf, Ralf; Weihe, Eberhard; Bette, Michael

doi:10.1158/1078-0432.ccr-14-0677

Cited by 23 publications

(33 citation statements)

References 51 publications

(57 reference statements)

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“…The induction of antiviral cytokines such as IFN and the modulation of pro-inflammatory cytokines as IL-6, have been demonstrated by ozonating blood such as major autohemotherapy (MAH). Although ozone is a weak inducer, reinfused lymphocytes and monocytes during mayor autohemotherapy (MAH), can migrate through the lymphoid system, and activate other cells that, in time, will lead to the stimulation of the immune system [32,34]. This may represent an important process because it is known that an acute viral disease becomes more severe because the virus is particularly virulent, the heterogeneous viral population evolves rapidly and escapes immune control, or because the immune system becomes tolerant to viral antigens and becomes unable to counteract the infection.…”

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confidence: 99%

Potential Cytoprotective Activity of Ozone Therapy in SARS-CoV-2/COVID-19

2020

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1) Background: The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease in China at the end of 2019 has caused a large global outbreak. Systemic ozone therapy (OT) could be potentially useful in the clinical management of several complications secondary to SARS-CoV-2. The rationale and mechanism of action has already been proven clinically in other viral infections and has been shown in research studies to be highly effective at decreasing organ damage mediated by inflammation and oxidative stress. This review summarizes the OT studies that illustrate the possible cytoprotective mechanism of action of ozone and its physiological by-products in target organs affected by SARS-CoV-2. (2) Methods: This review encompasses a total of 74 peer-reviewed original articles. It is mainly focused on ozone as a modulator of the NF-κB/Nrf2 pathways and IL-6/IL-1β expression. (3) Results: In experimental models and the few existent clinical studies, homeostasis of the free radical and antioxidant balance by OT was associated with a modulation of NF-κB/Nrf2 balance and IL-6 and IL-1β expression. These molecular mechanisms support the cytoprotective effects of OT against tissue damage present in many inflammatory diseases, including viral infections. (4) Conclusions: The potential cytoprotective role of OT in the management of organ damage induced by COVID-19 merits further research. Controlled clinical trials are needed.

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confidence: 99%

Potential Cytoprotective Activity of Ozone Therapy in SARS-CoV-2/COVID-19

2020

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“…The animal experiment was approved by the Regional Board Giessen, Germany [V54-19c20-15(1) MR20/26 Nr.34/2011] according to the German Animal Protection Law. Auricular tumors were experimentally induced by inoculation of a VX2 tumor cell suspension as described previously (11). Auricular VX2 tumor samples were randomly taken from two adult male Iffa Credo New Zealand White rabbits (Charles River Wiga Deutschland GmbH, Sulzfeld, Germany).…”

Section: Methodsmentioning

confidence: 99%

Virome Analysis Reveals No Association of Head and Neck Vascular Anomalies with an Active Viral Infection

Franke

Bette

Marquardt

et al. 2018

In Vivo

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Background/Aim: Vascular anomalies encompass different vascular malformations [arteriovenous (AVM), lymphatic (LM), venous lymphatic (VLM), venous (VM)] and vascular tumors such as hemangiomas (HA). The pathogenesis of vascular anomalies is still poorly understood. Viral infection was speculated as a possible underlying cause. Materials and Methods: A total of 13 human vascular anomalies and three human skin control tissues were used for viral analysis. RNA derived from AVM (n=4) and normal skin control (n=3) tissues was evaluated by RNA sequencing. The Virome Capture Sequencing Platform for Vertebrate Viruses (VirCapSeq-VERT) was deployed on 10 tissues with vascular anomalies (2×AVM, 1×HA, 1×LM, 2×VLM, 4×VM). Results: RNA sequencing did not show any correlation of AVM with viral infection. By deploying VirCapSeq-VERT, no consistent viral association was seen in the tested tissues. Conclusion: The analysis does not point to the presence of an active viral infection in vascular anomalies. However, transient earlier viral infections, e.g. during pregnancy, cannot be excluded with this approach.

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“…For nearly 20 years, the auricular VX2 carcinoma of the New Zealand White (NZW) rabbit serves as an animal model for human head and neck squamous cell carcinomas (HNSCC) (1). Our previous studies (2,3) demonstrated the suitability of the VX2 carcinoma as a model system for HPV associated HNSCC since this tumor, similarly to HPV + HNSCC, is the consequence of a papillomavirus (cottontail rabbit papilloma virus, CRPV) infection. Against this background it is of utmost importance to develop a VX2 carcinoma derived cell line to be able to perform in vitro studies with this animal model.…”

Section: Figure 3 Characterization Of the Vx2 Cell Line By Flow Cytomentioning

confidence: 99%

Establishment and characterization of a VX2 carcinoma derived rabbit cell line as a model system for the study of papilloma virus associated head and neck cancer

Ali

Ambreen

Pinnapireddy³

et al. 2019

Abstract- Und Posterband – 90. Jahresversammlung Der Deutschen Gesellschaft Für HNO-Heilkunde, Kopf- Und Hals-Chirurgie e.V., B

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A B C Figure 3. Characterization of the VX2 cell line by flow cytometry and Western blot analysis. (A) Two distinct populations of VX2 cells are observed during FACS analysis. One population was designated as small and the other was termed large based on their different cell sizes and granularity. (B) After sorting each of the two populations into a single tissue culture flask both subpopulations reappeared during further culturing pointing them to represent two phenotypes of the same cell line. (C) Western Blot analysis of unsorted VX2 cells as well as the small and the large populations demonstrated expression of the epidermal growth factor receptor (EGFR) with pronounced activity of this signaling pathway (Erk1/2-phospho) in the large population. EGFR: epidermal growth factor receptor, TrkB: tropomyosin-related kinase B, Erk1/2-phospho: extracellular signal regulated kinase 1 and 2 (active form), CXCR4: C-X-C chemokine receptor type 4 (=CD184), PCNA: proliferating cell nuclear antigen, Pin1: peptidyl-prolyl cis-trans isomerase 1.

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Intraperitoneal Oxidative Stress in Rabbits with Papillomavirus-Associated Head and Neck Cancer Induces Tumoricidal Immune Response That Is Adoptively Transferable

Cited by 23 publications

References 51 publications

Potential Cytoprotective Activity of Ozone Therapy in SARS-CoV-2/COVID-19

Potential Cytoprotective Activity of Ozone Therapy in SARS-CoV-2/COVID-19

Virome Analysis Reveals No Association of Head and Neck Vascular Anomalies with an Active Viral Infection

Establishment and characterization of a VX2 carcinoma derived rabbit cell line as a model system for the study of papilloma virus associated head and neck cancer

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