Intraperitoneal Oil Application Causes Local Inflammation with Depletion of Resident Peritoneal Macrophages

Alsina-Sanchís, Elisenda; Mülfarth, Ronja; Moll, Iris; Mogler, Carolin; Rodríguez-Vita, Juan; Fischer, Andreas

doi:10.1158/1541-7786.mcr-20-0650

Cited by 22 publications

(23 citation statements)

References 39 publications

(43 reference statements)

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“…Another important consideration is how to reconstitute and administer curcumenol, as it is insoluble in water. Therefore, dMSO was used in the present study to pre-dissolve curcumenol, which was then diluted in non-pharmaceutical grade corn oil, which showed little adverse effect during intraperitoneal injection in mice (53,54). With regard to the administration method, intraperitoneal injection was selected rather than intraarticular administration, based on the following: i) Intraperitoneal injection of corn oil is widely used in mouse models and the only administration method of curcumenol in mice appears to be intraperitoneal injection (55); and ii) there is a possibility of cartilage injury (56,57) and difficulties associated with injecting corn oil via multiple micro-injections into the knee joint.…”

Section: Discussionmentioning

confidence: 99%

Curcumenol mitigates chondrocyte inflammation by inhibiting the NF‑κB and MAPK pathways, and ameliorates DMM‑induced OA in mice

et al. 2021

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At present, an increasing number of individuals are affected by osteoarthritis (OA), resulting in a heavy socioeconomic burden. OA in knee joints is caused by the release of inflammatory cytokines and subsequent biomechanical and structural deterioration. To determine its anti-inflammatory function, the current study investigated the use of the plant-derived medicine, curcumenol, in OA treatment. curcumenol was not cytotoxic to ATdc5 chondrocytes and primary chondrocytes, as determined using a cell viability test. When these cells were treated with TNF-α and IL-1β to induce inflammation, curcumenol treatment inhibited the progression of inflammation by inactivating the NF-κB and MAPK signaling pathways, as well as decreasing the expression levels of MMP3 (as indicated by reverse transcription-quantitative PcR and western blotting). Moreover, to analyze metabolic and catabolic status in high-density and pellet culture, catalytic changes and the degradation of the extracellular matrix induced by TNF-α and IL-1β, were evaluated by alcian blue staining. These catalytic deteriorations were ameliorated by curcumenol. Using curcumenol in disease management, the mechanical and metabolic disruption of cartilage caused in the destabilization of medial meniscus (dMM) model was prevented in vivo. Thus, curcumenol mitigated inflammation in ATdc5 chondrocytes and primary mice chondrocytes, and also ameliorated OA in a dMM-induced mouse model.

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Section: Discussionmentioning

confidence: 99%

Curcumenol mitigates chondrocyte inflammation by inhibiting the NF‑κB and MAPK pathways, and ameliorates DMM‑induced OA in mice

et al. 2021

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“…Oil, if used for intraperitoneal administration, can cause an inflammatory response in mice, with severity depending on the type of oil used. Mineral and peanut oil have the highest inflammatory response while corn and olive oil produce less of a reaction (Alsina-Sanchis et al, 2021). Inflammation caused by oil injections may interfere with immune cell recruitment and the overall immune response (Alsina-Sanchis et al, 2021).…”

Section: Vehiclementioning

confidence: 99%

“…Mineral and peanut oil have the highest inflammatory response while corn and olive oil produce less of a reaction (Alsina-Sanchis et al, 2021). Inflammation caused by oil injections may interfere with immune cell recruitment and the overall immune response (Alsina-Sanchis et al, 2021). Analysis of the immune response to endometriosis may be confounded by the inflammatory response elicited by the presence of oil in the peritoneal cavity (Cummings and Metcalf, 1995;Hirata et al, 2005;Cheng et al, 2011;Kulak et al, 2011;Wilkosz et al, 2011;Burns et al, 2012Burns et al, , 2018Wieser et al, 2012;Naqvi et al, 2014;Sharma et al, 2021).…”

Section: Vehiclementioning

confidence: 99%

Endometriosis in the Mouse: Challenges and Progress Toward a ‘Best Fit’ Murine Model

et al. 2022

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Endometriosis is a prevalent gynecologic condition associated with pelvic pain and infertility characterized by the implantation and growth of endometrial tissue displaced into the pelvis via retrograde menstruation. The mouse is a molecularly well-annotated and cost-efficient species for modeling human disease in the therapeutic discovery pipeline. However, as a non-menstrual species with a closed tubo-ovarian junction, the mouse poses inherent challenges as a preclinical model for endometriosis research. Over the past three decades, numerous murine models of endometriosis have been described with varying degrees of fidelity in recapitulating the essential pathophysiologic features of the human disease. We conducted a search of the peer-reviewed literature to identify publications describing preclinical research using a murine model of endometriosis. Each model was reviewed according to a panel of ideal model parameters founded on the current understanding of endometriosis pathophysiology. Evaluated parameters included method of transplantation, cycle phase and type of tissue transplanted, recipient immune/ovarian status, iterative schedule of transplantation, and option for longitudinal lesion assessment. Though challenges remain, more recent models have incorporated innovative technical approaches such as in vivo fluorescence imaging and novel hormonal preparations to overcome the unique challenges posed by murine anatomy and physiology. These models offer significant advantages in lesion development and readout toward a high-fidelity mouse model for translational research in endometriosis.

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“…Incomplete recombination due to suboptimal 4-OHT administration or different bioavailability in different organs [117,118] may also affect the efficiency of genetic lineage tracing. Conversely, deleterious effects to the embryo occur from the toxicity of high levels of tamoxifen, 4-OHT or their delivery vehicles [119][120][121]. Toxicity can also arise when Cre recognises genomic sequences resembling loxP sites to cause DNA breaks [122], especially when Cre is expressed highly, or when random genomic insertion of transgenes disrupts essential endogenous genetic loci [123].…”

Section: Dermal Capillary Plexusmentioning

confidence: 99%

Mechanisms and cell lineages in lymphatic vascular development

et al. 2021

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Lymphatic vessels have critical roles in both health and disease and their study is a rapidly evolving area of vascular biology. The consensus on how the first lymphatic vessels arise in the developing embryo has recently shifted. Originally, they were thought to solely derive by sprouting from veins. Since then, several studies have uncovered novel cellular mechanisms and a diversity of contributing cell lineages in the formation of organ lymphatic vasculature. Here, we review the key mechanisms and cell lineages contributing to lymphatic development, discuss the advantages and limitations of experimental techniques used for their study and highlight remaining knowledge gaps that require urgent attention. Emerging technologies should accelerate our understanding of how lymphatic vessels develop normally and how they contribute to disease.

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Intraperitoneal Oil Application Causes Local Inflammation with Depletion of Resident Peritoneal Macrophages

Cited by 22 publications

References 39 publications

Curcumenol mitigates chondrocyte inflammation by inhibiting the NF‑κB and MAPK pathways, and ameliorates DMM‑induced OA in mice

Curcumenol mitigates chondrocyte inflammation by inhibiting the NF‑κB and MAPK pathways, and ameliorates DMM‑induced OA in mice

Endometriosis in the Mouse: Challenges and Progress Toward a ‘Best Fit’ Murine Model

Mechanisms and cell lineages in lymphatic vascular development

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