Intraperitoneal mesenchymal stem cell administration ameliorates allergic rhinitis in the murine model

Abstract

Previous studies showed that bone marrow-derived mesenchymal stem cells (BMSCs) could ameliorate a variety of immune-mediated and inflammatory diseases due to their immunomodulatory and anti-inflammatory effects. In this study, we developed a mouse model of ovalbumin (OVA) induced allergic inflammation in the upper airways and evaluated the effects of the intraperitoneal administration of BMSCs on allergic inflammation. Twenty-five BALB/c mice were divided into five groups; group I (control group), group II (sensitized and challenged with OVA and treated with saline-placebo group), group III (sensitized and challenged with OVA and treated with 1 × 10 BMSCs), group IV (sensitized and challenged with OVA and treated with 2 × 10 BMSCs), and group V (sensitized and challenged with phosphate buffered saline (PBS) and treated with 1 × 10 BMSCs). Histopathological features (number of goblet cells, eosinophils and mast cells, basement membrane, epithelium thickness, and subepithelial smooth muscle thickness) of the upper and lower airways and BMSCs migration to nasal and lung tissue were evaluated using light and confocal microscopes. Levels of cytokines in the nasal lavage fluid and lung tissue supernatants were measured using enzyme-linked immunosorbent assay (ELISA). Confocal microscopic analysis showed that there was no significant amount of BMSCs in the nasal and lung tissues of group V. However, significant amount of BMSCs were observed in group III and IV. In OVA-induced AR groups (group II, III, and IV), histopathological findings of chronic asthma, such as elevated subepithelial smooth muscle thickness, epithelium thickness, and number of goblet and mast cells, were determined. Furthermore, the number of nasal goblet and eosinophil cells, histopathological findings of chronic asthma, and IL-4, IL-5, IL-13, and NO levels was significantly lower in both BMSCs-treated groups compared to the placebo group. Our findings indicated that histopathological findings of chronic asthma were also observed in mice upon AR induction. BMSCs migrated to the nasal and lung tissues following intraperitoneal delivery and ameliorated to the airway remodeling and airway inflammation both in the upper and lower airways via the inhibition of T helper (Th) 2 immune response in the murine model of AR.

Unraveling the therapeutic effects of mesenchymal stem cells in asthma

“…It has been shown that intraperitoneal administration of bone marrow MSCs is the potential to modulate the allergic asthma reaction. Soon after injection into the peritoneal cavity, these cells easily migrate to the pulmonary niche and exert immunomodulatory capacity [ 73 , 74 ].

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Protective effect of miR‐138‐5p inhibition modified human mesenchymal stem cell on ovalbumin‐induced allergic rhinitis and asthma syndrome

“…MSCs are capable of differentiating into a number of cell types and are of high translation potential for clinical applications. MSCs are found to migrate to the airway under inflammation and exert reparative function 11‐13 . A number of previous studies have demonstrated the great potential of MSCs in treating AR 9,11,14‐16 .…”

Mesenchymal Stem Cell-Based Therapy for Allergic Rhinitis

“…A similar discovery was made in a separate study. It was found that bone marrow-derived MSCs migrated to the nasal and lung tissues following intraperitoneal delivery and ameliorated to the airway remodeling and airway inflammation both in the upper and lower airways via the inhibition of Th2 immune response in the mouse model of AR [40]. [41].…”