Intraperitoneal Injection of Mice

Miner, Norman; Koehler, Jacqueline A.; Greenaway, Laura

doi:10.1128/am.17.2.250-251.1969

Cited by 35 publications

(4 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both intragastric and intraperitoneal delivery are similar in terms of severity for the animal and technical difficulty, while intravenous administration is usually more difficult as well as more stressful for the mice 32 . Oral administration further eliminates the 10-20% rate of error during intraperitoneal injections into the intestinal lumen or the stomach, which may impact the rate of glucose delivery and redistribution 33,34 .…”

mentioning

confidence: 99%

Study of <em>In Vivo</em> Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Nagy

Einwallner

2018

JoVE

116

View full text Add to dashboard Cite

Obesity represents the most important single risk factor in the pathogenesis of type 2 diabetes, a disease which is characterized by a resistance to insulin-stimulated glucose uptake and a gross decompensation of systemic glucose metabolism. Despite considerable progress in the understanding of glucose metabolism, the molecular mechanisms of its regulation in health and disease remain under-investigated, while novel approaches to prevent and treat diabetes are urgently needed. Diet derived glucose stimulates the pancreatic secretion of insulin, which serves as the principal regulator of cellular anabolic processes during the fed-state and thus balances blood glucose levels to maintain systemic energy status. Chronic overfeeding triggers meta-inflammation, which leads to alterations in peripheral insulin receptor-associated signaling and thus reduces the sensitivity to insulin-mediated glucose disposal. These events ultimately result in elevated fasting glucose and insulin levels as well as a reduction in glucose tolerance, which in turn serve as important indicators of insulin resistance. Here, we present a protocol for the generation and metabolic characterization of high-fat diet (HFD)-fed mice as a frequently used model of diet-induced insulin resistance. We illustrate in detail the oral glucose tolerance test (OGTT), which monitors the peripheral disposal of an orally administered glucose load and insulin secretion over time. Additionally, we present a protocol for the insulin tolerance test (ITT) to monitor whole-body insulin action. Together, these methods and their downstream applications represent powerful tools to characterize the general metabolic phenotype of mice as well as to specifically assess alterations in glucose metabolism. They may be especially useful in the broad research field of insulin resistance, diabetes and obesity to provide a better understanding of pathogenesis as well as to test the effects of therapeutic interventions.

show abstract

mentioning

confidence: 99%

Study of <em>In Vivo</em> Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Nagy

Einwallner

2018

JoVE

116

View full text Add to dashboard Cite

show abstract

“…Implement IP injection was as previously described. 15 Restrain mice by gripping the skin of the posterior surface of the body and the tail. Inject conivaptan or vehicle into the left lower quadrant of the abdomen using a 26 G/1/2 inch needle.…”

Section: Installation Of IV Catheter Into the Jugular Vein For Contin...mentioning

confidence: 99%

Continuous IV Infusion is the Choice Treatment Route for Arginine-vasopressin Receptor Blocker Conivaptan in Mice to Study Stroke-evoked Brain Edema

Zeynalov¹,

Jones²,

Elliott

2016

JoVE

View full text Add to dashboard Cite

Stroke is one of the major causes of morbidity and mortality in the world. Stroke is complicated by brain edema and other pathophysiological events. Among the most important players in the development and evolution of stroke-evoked brain edema is the hormone arginine-vasopressin and its receptors, V1a and V2. Recently, the V1a and V2 receptor blocker conivaptan has been attracting attention as a potential drug to reduce brain edema after stroke. However, animal models which involve conivaptan applications in stroke research need to be modified based on feasible routes of administration. Here the outcomes of 48 hr continuous intravenous (IV) are compared with intraperitoneal (IP) conivaptan treatments after experimental stroke in mice. We developed a protocol in which middle cerebral artery occlusion was combined with catheter installation into the jugular vein for IV treatment of conivaptan (0.2 mg) or vehicle. Different cohorts of animals were treated with 0.2 mg bolus of conivaptan or vehicle IP daily. Experimental stroke-evoked brain edema was evaluated in mice after continuous IV and IP treatments. Comparison of the results revealed that the continuous IV administration of conivaptan alleviates post-ischemic brain edema in mice, unlike the IP administration of conivaptan. We conclude that our model can be used for future studies of conivaptan applications in the context of stroke and brain edema.

show abstract

“…All anesthetic regimens were administered to study animals via a single intraperitoneal injection in the lower-right quadrant of the abdomen using a 26-gauge 1 mL syringe (Miner et al, 1969;Coria-Avila et al, 2007). Prior to administration, animals were weighed and doses of anesthetic agents were determined on a mg/kg basis.…”

Section: Anesthetic Administration and Monitoringmentioning

confidence: 99%

Evaluation on Efficacy and Safety of Tribromoethanol and Tribromoethanol plus α₂-Adrenergic Agonists in Different Mouse Strains

et al. 2010

View full text Add to dashboard Cite

The present study was carried out to provide a guideline for injecting tribromoethanol (TBE) as the main anesthetic agent, while adjusting the doses of xylazine (X) and medetomidine (M) according to different strains of mice (male ICR, C57BL/6, and BALB/c). Seven intraperitoneal injection anesthesia protocols using TBE and mixtures of TBE and α 2 -adrenergic agonists (TBE/X and TBE/M) were compared in terms of their efficacy and safety (anesthetic duration, death rate, and the development of pathological lesions of abdominal organs). All animals that were injected with a low dose of TBE (200 mg/kg) displayed clear signs of light anesthesia with a strong pedal withdrawal reflex. Despite the good anesthetic effect, a high dose of TBE (400 mg/kg) was not a suitable anesthetic for major surgery in all mouse strains because of the risk of pathologic changes in the abdominal organs, such as retention of the digestive tract, peritonitis, and fibrinoid adhesion. TBE200/X10 and TBE200/M0.5 (TBE, 200 mg/kg; X, 10 mg/kg; M, 0.5 mg/kg) appeared to be safe and provided satisfactory anesthesia in ICR mice. Finally, there were clear differences in anesthetic efficacy among ICR, C57BL/6, and BALB/c strains. TBE/M and TBE/X did not anesthetize BALB/c mice, and it anesthetized C57BL/6 mice for a short time. When administered with TBE/X and TBE/M maintained the sedation of ICR mice. We were able to establish different regimes for each strain (TBE200/X20 for C57BL/6, TBE300/X10 and TBE200/M1 for BALB/c). Our results showed that TBE/X and TBE/M could be recommended as an anesthetic mixture, with the dose appropriately adjusted according to mouse strain.

show abstract

Intraperitoneal Injection of Mice

Cited by 35 publications

References 1 publication

Study of <em>In Vivo</em> Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Study of <em>In Vivo</em> Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Continuous IV Infusion is the Choice Treatment Route for Arginine-vasopressin Receptor Blocker Conivaptan in Mice to Study Stroke-evoked Brain Edema

Evaluation on Efficacy and Safety of Tribromoethanol and Tribromoethanol plus α₂-Adrenergic Agonists in Different Mouse Strains

Contact Info

Product

Resources

About

Intraperitoneal Injection of Mice

Cited by 35 publications

References 1 publication

Study of <em>In Vivo</em> Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Study of <em>In Vivo</em> Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Continuous IV Infusion is the Choice Treatment Route for Arginine-vasopressin Receptor Blocker Conivaptan in Mice to Study Stroke-evoked Brain Edema

Evaluation on Efficacy and Safety of Tribromoethanol and Tribromoethanol plus α2-Adrenergic Agonists in Different Mouse Strains

Contact Info

Product

Resources

About

Evaluation on Efficacy and Safety of Tribromoethanol and Tribromoethanol plus α₂-Adrenergic Agonists in Different Mouse Strains