Intraperitoneal Injection of Mice

Abstract

Steward, Ornellas, Beernink, and Northway (2) reported a 14% error in the placement of intraperitoneal injections of mice. They considered this error inherent in the technique and not simply correctable. We similarly found an error in the intraperitoneal injection of mice and attempted to identify the cause of error by varying such technical procedures as size of needle, site of penetration (through lower left versus lower right quadrant), investigator, angle of needle to the abdominal wall, and speed of injection. None of these technical modifications consistently eliminated or reduced the error of placement.

<p>Guidelines and Considerations for Metabolic Tolerance Tests in Mice</p>

“…23 It is technically a simple procedure to perform; however, training and competent lab personnel should ensure that substances are delivered accurately and that needle perforation of the gut or stomach lumen is avoided. 23,24 In the protocols presented below the i.p. route of administration has been described as a basic method.…”

Rapid Detection of Clostridium botulinum Toxin by Capillary Tube Diffusion

“…Injection of products directly into mice sometimes leads to "nonspecific" deaths due to properties of the product itself or bacterial products other than botulinal toxin. Deaths can also be caused by piercing vital organs during inoculation (12,18). The capillary method detected toxin in inoculated cans at levels corresponding to 1 x 102 to 1.5 x 104 MLD/ml in the mouse bioassay provided that the clam juice was treated with Sephadex before use.…”

Androcin, a novel type of cysteine‐rich venom peptide from Androctonus bicolor, induces akinesia and anxiety‐like symptoms in mice

“…Mice were injected intraperitoneally with recombinant Androcin as described previously . Negative control mice were injected with the GST protein, whereas blank control mice were untreated.…”