Intraperitoneal gene therapy with adenoviral-mediated p53 tumor suppressor gene for ovarian cancer model in nude mouse

Abstract

In an effort to develop a method for better local control of advanced ovarian cancers, we have established a peritoneal tumor model of ovarian cancer in the nude mouse and applied intraperitoneal gene therapy with the recombinant adenoviral-mediated wild-type p53 tumor suppressor gene (Avp53). The results indicate that: (a) the recombinant adenoviral vector system effectively infected the tumor and normal cells in the peritoneal cavity; and (b) Avp53 treatment effectively suppressed the growth of peritoneal tumors and prolonged the survival of the treated group, especially when the tumor burden was less. These results suggest that intraperitoneal gene therapy using Avp53 is potentially useful as an adjuvant therapeutic modality in human ovarian cancer.

Interferon regulatory factor-1 (IRF-1) is a mediator for interferon-γ induced attenuation of telomerase activity and human telomerase reverse transcriptase (hTERT) expression

“…Adenovirus was generated as described by Kim et al (1999). Briefly, pDACMV-IRF-1 was generated by the insertion of 1.8 kb EcoR1 fragment of human IRF-1 from pVL1932-IRF-1 (kind gift from Dr Takashi Fujita, The Tokyo Metropolitan Institute of Medical Science, Japan) into pDACMVp(A).…”

Polyethylenimine-mediated gene transfer into pancreatic tumor dissemination in the murine peritoneal cavity

“…cer model, 9 and the administration of BAX-expressing adenovirus was capable of eradicating Ͼ99% of ovarian tumor implants. 10 A phase I trial suggested that adenovirus-mediated gene therapy using anti-erbB2-directed intrabody is feasible in the human ovarian cancer.…”

Determination of molecules regulating gene delivery using adenoviral vectors in ovarian carcinomas

“…In ongoing studies, the replicationdeficient adenovirus type 5 is mostly favored over alternatively available viral vector systems for gene delivery into tumor cells. [1][2][3][4] Due to its ability to transfer genes into non-dividing cells this highly infectious adenoviral system was found to be also suitable for treatment of malignancies characterized by a low mitotic index.…”