Intraperitoneal delivery of hrR3 and ganciclovir prolongs survival in mice with disseminated pancreatic cancer

Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

“…Ovarian, pancreatic, and gastric cancers are particularly attractive targets for IP injections. [6,37,[39][40][41][42][43][44][45][46] In this study, for the first time, we comprehensively compare the transgene expression generated by IP injection of branched or linear PEI-pDNA nanoparticles using bioluminescent imaging (BLI). BLI is a modern transgene expression imaging technique from which the results strongly correlate to transgene expression results measured using traditional tissue and organ harvesting methodologies.…”

Polyethylenimine-mediated gene transfer into pancreatic tumor dissemination in the murine peritoneal cavity

“…[5][6][7][8] As regards an apoptosis-inducing gene therapy, the treatment of adenovirus encoding p53 gene effectively suppressed the growth of peritoneal tumors in ovarian can-more efficient than the cationic lipids examined in this study in vivo, and the transgene was preferentially expressed in the tumors. Although PCR analysis showed that the injected DNA was delivered to various organs, the distributed DNA became undetectable by 6 months after the gene transfer.…”

Oncolytic Herpes Viruses as a Potential Mechanism for Cancer Therapy

“…An additional advantage of the deletion of RR is hypersensitivity of this virus to GCV, compared to the parent wild-type strain KOS (90). Long-term survival of the animals was found to be increased dramatically when GCV was administered in addition to viral treatment, with increases of 30 Á/60% over virus alone (117,118); administration of GCV also eliminated viral vector-related mortality in one case (117). Replication of this virus was enhanced in the presence of cyclophosphamide, which, in addition to its anti-cancer activity, is a strong immunosuppressive agent.…”

“…An RR-deficient strain, hrR3, has been tested in animal models of cancers of the brain (89,90,116,117), pancreas (118), and liver (109), demonstrating significant virus-induced cytotoxicity and necrosis of tumors. An additional advantage of the deletion of RR is hypersensitivity of this virus to GCV, compared to the parent wild-type strain KOS (90).…”