Intraperitoneal Administration of Muramyl Dipeptide β-Heptylglycoside to Pregnant and Non-Pregnant Female Mice Modulates Production of Th1/Th2/Th17/Tr1 Cytokines by Splenocytes Ex Vivo

Abstract

Muramyl dipeptide β-heptylglycoside (C7MDP) was administered to non-pregnant CBA female mice and pregnant mice after non-abortion-prone mating (CBA×BALB/c) and mating associated with a high rate of spontaneous abortion (CBA×DBA/2). In non-pregnant females, C7MDP increased the production of IL-2, IL-4, IL-5, IL-6, IL-17, IFNγ, TNFα, and GM-CSF at constant production of IL-1α and IL-10. C7MDP increased the production of IL-10 and IL-17 and suppressed the production of IFNγ on day 8 of gestation in non-abortion-prone mouse couples and stimulated the synthesis of IL-4 and IFNγ, reduced IL-5 production, and slightly increased IL-1α secretion after abortion-prone mating. On day 14 of gestation, C7MDP elevated the yield of IL-2, IL-4, IFNγ, TNFα, and GM-CSF in CBA×BALB/c and CBA×DBA/2 couples and IL-17 in the fi rst variant of mating.

Features of the functioning of the natural defense mechanisms of piglets under the influence of immunotropic substances

“…Under the TIR effect, phagocytic cells are activated, the number of lymphocytes is normalised, cytokine synthesis is enhanced, etc. (Kalyuzhin et al, 2015). Thus, in order to correct the mammalian immune system state during immunosuppressive periods of organism development, it is necessary to use immunotropic drugs that directly or indirectly affect the resistance mechanisms and allow to ensure their optimally high level, and thus reduce the risk of infectious diseases in animals (Srinivasan & Babensee, 2020).…”