Intrapericardial administration of novel DNA formulations based on thermosensitive Poloxamer 407 gel

Potential of gene therapy as a treatment for heart failure

“…This limitation can be partly overcome by the co-administration of various pharmacological agents. Proteolytic enzymes and polyethyleneimine have been shown to increase the penetration depth of the vectors and to allow progressive release, often at the cost of cardiac toxicity (36,40). Although vectors are injected into a closed space, some studies reported extracardiac gene expression, probably due to the rapid turnover of the pericardium fluid through the lymphatic absorption (41).…”

Sustained release of 5-FU from Poloxamer gels interpenetrated by crosslinking chitosan network

“…At a sufficiently high concentration and temperature (higher than LCST, lower critical solution temperature), the Poloxamer micelles pack in an order that results in a transition of sol to a gel state called thermo-sensitive gel (Juhasz et al, 1989;Liu and Chu, 2000;Dumortier et al, 2006;Ruel-Gariepy and Leroux, 2006). Numerous researchers have examined the thermo-sensitivity of Poloxamer (P) gels with an interest in their potential use in various pharmaceutical applications (Liu and Chu, 2000;Amiji et al, 2002;Roques et al, 2007;Caffaggi et al, 2008). For example, injecting Paclitaxel loaded Poloxamer 407 (20%) into a tumor positively inhibits tumor growth (Amiji et al, 2002).…”

“…For example, injecting Paclitaxel loaded Poloxamer 407 (20%) into a tumor positively inhibits tumor growth (Amiji et al, 2002). Poloxamer 407 has also been employed as a carrier for the intra-pericardial administering of plasmid DNA in gene therapy (Roques et al, 2007). However, P gels such as Poloxamer 188 and 407 generally sustain the release of drugs for only a short period (such as <5 h) in an aqueous environment (Barichello et al, 1999;Jeong et al, 2002;Ricci et al, 2005), since P gels dissociate rapidly in (such an OR an aqueous) environment.…”

Targeting Sarcoplasmic Reticulum Calcium ATPase by Gene Therapy

“…However, the structural architecture of the visceral pericardium can limit transfection efficiency of superficial myocardial layers (Ladage et al, 2011). Addition of permeabilizing agents can increase delivery using a pericardial delivery approach; however, the toxicity and clinical feasibility of these agents has not been well elucidated (Roques et al, 2007;Karakikes et al, 2012). Mechanical methods utilizing microbubbles combined with echocardiography has been shown to provide efficient gene transduction of the myocardium as well (Beeri, 2002).…”