Intraocular vaccination with an inactivated highly pathogenic avian influenza virus induces protective antibody responses in chickens

Hikono, Hirokazu; Mase, Masaji; Matsuu, Aya; Nakayama, Megumi; Saito, Takehiko

doi:10.1016/j.vetimm.2012.10.005

Cited by 14 publications

(16 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In mammals, dendritic cells in mucosa invoke immune responses. In chicken, intraocular rather than intranasal vaccination facilitates protection against avian influenza . Optimal conditions were determined in our previous study .…”

Section: Resultsmentioning

confidence: 99%

“…In chicken, intraocular rather than intranasal vaccination facilitates protection against avian influenza . Optimal conditions were determined in our previous study . Because it has not yet been shown that there are immunocompetent cells in the conjunctiva of chickens , amounts of cytokine in the conjunctiva and other tissue were determined after Pam2 or polyI:C had been administered in the form of eye drops.…”

Section: Resultsmentioning

confidence: 99%

“…Optimal conditions were determined in our previous study . Because it has not yet been shown that there are immunocompetent cells in the conjunctiva of chickens , amounts of cytokine in the conjunctiva and other tissue were determined after Pam2 or polyI:C had been administered in the form of eye drops. Because no definitive markers of myeloid cells have yet been identified in chickens , their Harderian glands, lungs and spleens were harvested to quantify mRNA after instillation.…”

Section: Resultsmentioning

confidence: 99%

“…Hikono et al . reported a novel strategy for vaccination against highly pathogenic avian influenza; however, this vaccination method is insufficiently effective for practical use (the survival rate is only 75%) . S.c. or i.m.…”

Section: Comparison Of Relevant Human and Chicken Prrsmentioning

confidence: 99%

See 3 more Smart Citations

Cytokine responses to eye spray adjuvants for enhancing vaccine‐induced immunity in chickens

Takaki

Sato

Kurata

et al. 2016

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

Eye spray influenza vaccines for chickens are increasingly available; however, how to enhance cellular and antibody responses to them remains undetermined. Here, eye-drops containing the immune-enhancing adjuvants Pam2CSK4 or polyI:C were assessed in chickens. Application of these TLR agonists to chicken conjunctiva resulted in up-regulation of IL-1β, but not other cytokines, including IFN and IL-6, in the spleen, lung and Harderian gland. Thus, responses to adjuvant applied to the conjunctival mucosa of chickens differ from those expected from the responses to intra-nasal adjuvants in mammals. Identifying an appropriate delivery route for adjuvants is crucial for evoking immune responses in chickens.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Comparison Of Relevant Human and Chicken Prrsmentioning

confidence: 99%

See 2 more Smart Citations

Cytokine responses to eye spray adjuvants for enhancing vaccine‐induced immunity in chickens

Takaki

Sato

Kurata

et al. 2016

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previously, it was suggested, that class B CpG ODN, administered by the eye-drop route, have the potential to act as mucosal vaccine adjuvants (Hikono et al 2013). The ability to induce a potent Th1-type immune response is of considerable importance, because in the infection with various pathogens, cell-mediated immunity is correlated with protection (Patel et al 2008, Taghavi et al 2008.…”

Section: Discussionmentioning

confidence: 99%

The effects of subcutaneous and intraocular administration of class B ODN CpG in chicken on the expression of TLR21, IFN-γ and IL-1β

Chrząstek¹,

Wieliczko²

2014

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The synthetic unmethylated oligodeoxynucleotides with CpG motifs (CpG ODN) were shown to activate Toll-like receptor 21 (TLR21) and stimulate the innate and adaptive immune system. In this study we tested the expression of TLR21, interferon (IFN)-γ and interleukin (IL)-1β mRNA in the blood after subcutaneous and intraocular application of the class B CpG ODN in chicken. The relative expression of mRNA of TLR21, IFN-γ and IL-1β were quantified at 3, 6, 12, 24 and 72 h post-stimulation. The study revealed that IFN-γ mRNA expression was significantly upregulated 12 h after subcutaneous stimulation with a high and low dose of ODN CpG, whereas the IL-1β mRNA expression levels were significantly upregulated 3 and 72 h after subcutaneous administration. After intraocular administration, the IL-1β mRNA levels were the highest at 24 h post-application, albeit not specifically. This data indicates that class B CpG ODN has the ability to induce TLR21 response in blood when administered parenterally in chicken. In contrast, intraocular administration of CpG ODN was not able to produce a significant increase in cytokine mRNA expression in blood. The data suggest that additional stimulus, e.g. the antigen, may be needed on the site of mucosal administration to activate systemic immune response.

show abstract

Protection of Chinese painted quails (Coturnix chinensis) against a highly pathogenic H5N1 avian influenza virus strain after vaccination

et al. 2013

View full text Add to dashboard Cite

Chinese painted quails immunized with a single dose (6 μg HA) of inactivated H5N1 (clade 1) influenza vaccine NIBRG-14 and challenged with 100 LD50 of the heterologous A/Swan/Nagybaracska/01/06(H5N1) (clade 2.2) strain were protected, whereas unvaccinated quails died after challenge. No viral antigens or RNA were detected in cloacal swabs from immunized animals. Sera obtained post-immunization gave low titres in serological assays against the vaccine and the challenge viruses. Our results demonstrate the protective efficacy of the NIBRG-14 strain against the challenge virus and the usefulness of these small birds in protection studies of influenza vaccines.

show abstract

Intraocular vaccination with an inactivated highly pathogenic avian influenza virus induces protective antibody responses in chickens

Cited by 14 publications

References 39 publications

Cytokine responses to eye spray adjuvants for enhancing vaccine‐induced immunity in chickens

Cytokine responses to eye spray adjuvants for enhancing vaccine‐induced immunity in chickens

The effects of subcutaneous and intraocular administration of class B ODN CpG in chicken on the expression of TLR21, IFN-γ and IL-1β

Protection of Chinese painted quails (Coturnix chinensis) against a highly pathogenic H5N1 avian influenza virus strain after vaccination

Contact Info

Product

Resources

About