Intraocular Pressure Reduction in a Pigmentary Glaucoma Model by Goniotome Ab Interno Trabeculectomy

Wang, Chao; Dang, Yalong; Shah, Priyal; Esfandiari, Hamed; Hong, Ying; Loewen, Ralitsa T.; Waxman, Susannah; Atta, Sarah; Xia, Xiaobo; Loewen, Nils A.

doi:10.20944/preprints201806.0436.v1

Cited by 3 publications

(3 citation statements)

References 38 publications

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“…Dysfunction of TM leads to increased outflow resistance and elevated IOP. In earlier studies with primary porcine TM cells [3] and porcine anterior segment perfusion model [4] , we found that pigmentary debris caused an increase of TM cell stress fibers, reduced phagocytosis, and mobility, and led to a persistent IOP elevation [17] . Through gene microarray and signal pathway analysis, we found that the Rho pathway was activated in TM cells [3,4] .…”

Section: Discussionmentioning

confidence: 84%

Ripasudil in a model of pigmentary glaucoma

Wang

Dang

Waxman

et al. 2020

Preprint

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Purpose : To investigate the effects of Ripasudil (K-115), a Rho-kinase inhibitor, in a porcine model of pigmentary glaucoma.Methods: Hallmark features of trabecular meshwork (TM), the principle structure of the outflow system affected in this model, were analyzed. In vitro TM cells and ex vivo perfused eyes were subjected to pigment dispersion followed by K-115 treatment (PK115). PK115 was compared to sham-treated controls (C) and pigment (P). Cytoskeletal alterations were assessed by F-actin labeling.TM cell phagocytosis of fluorescent targets was evaluated by flow cytometry. Cell migration was studied with a wound-healing assay. Intraocular pressure was continuously monitored and compared to after the establishment of the pigmentary glaucoma model and after treatment with K-115.

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Section: Discussionmentioning

confidence: 84%

Ripasudil in a model of pigmentary glaucoma

Wang

Dang

Waxman

et al. 2020

Preprint

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“…Both increased age and reduced TM cellularity are a major risk factor for the development of glaucoma. On the other hand, partially removing TM cells can also reduce IOP at least temporarily 47 , while excision of TM causes a profound and lasting IOP drop ex vivo 27,48 and clinically 49 . Abu-Hassan et al 23 recently showed that the transplantation of human TM cells or TM-like iPSCs into the anterior segment perfusion model can restore outflow after killing about 1/3rd of TM cells with saponin.…”

Section: Discussionmentioning

confidence: 99%

Tissue-Engineered Anterior Segment Eye Cultures Maintain Intraocular Pressure

Waxman¹,

Wang²,

Dang³

et al. 2020

Preprint

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Glaucoma is a blinding disease largely caused by increased resistance to drainage of fluid from the eye’s anterior chamber, resulting in elevated intraocular pressure (IOP). A major site of fluid outflow regulation and pathology is the trabecular meshwork (TM) at the entrance of the eye’s drainage system. We aimed to characterize the structural and functional properties of a newly developed tissue-engineered anterior segment eye culture model. We hypothesized that repopulation of a decellularized TM with non-native TM cells could restore aspects of normal TM. The decellularization protocol removed all cells and debris while preserving the ECM. Seeded cells localized to the TM region and progressively infiltrated the meshwork ECM. Cells reached a distribution comparable to control TM after four days of perfusion culture. After a perfusion rate increase challenge, tissue-engineered cultures reestablished normal IOPs (reseeded = 13.7±0.4 mmHg, decellularized = 35.2±2.2 mmHg, p < 0.0001). eGFP expressing CrFK control cells caused a high and unstable IOP (27.0±6.2 mmHg). In conclusion, we describe a readily available, storable, and biocompatible scaffold for anterior segment perfusion culture of non-native cells. Tissue-engineered organs demonstrated similarities to native tissues and may reduce the need for scarce donor globes in outflow research.

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“…A 180-degree ab interno trabeculectomy was performed 24 hours later along the same nasal180° of trabecular meshwork. [26][27][28]…”

Section: Nd:yag-laser Tp and Aitmentioning

confidence: 99%

A Predictive Test of Outflow Enhancement by Ab Interno Trabeculectomy

Verma-Fuehring¹,

Dakroub²,

Han³

et al. 2021

Preprint

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Purpose: To investigate trabeculopuncture (TP) for predicting the outcome of ab interno trabeculectomy (AIT). AIT is an effective, low-risk procedure for open angle glaucoma. Despite widespread utilization, it fails in patients with an unidentified distal outflow resistance. Methods: We bisected 81 enucleated porcine eyes and perfused them for 72 hours. They were assigned to two groups: trial (n=42) and control (n=39). Intraocular pressure (IOP) was measured continuously. At 24 hours, four YAG-laser trabeculopunctures on the nasal trabecular meshwork were performed, followed by a 180° AIT at the same site at 48 hours. Eyes were divided into TP and AIT responders and non-responders; the proportion of TP responders between both AIT groups was compared. Results: Both post-TP and post-AIT IOPs were lower than baseline IOP (p=0.015 and p<0.01, respectively). The success rates of TP and AIT were 69% and 85.7%, respectively. The proportion of TP responders among AIT responders was greater than that of AIT non-responders (p<0.01). Sensitivity and specificity values of TP as predictive test for AIT success were 77.7% and 83.3%, respectively. The positive and negative predictive values were 96.6% and 38.5%, respectively. Conclusion: A 10% reduction in IOP after TP can be used as predictor for the success (>20% IOP decrease) of 180° AIT in porcine eyes.

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Intraocular Pressure Reduction in a Pigmentary Glaucoma Model by Goniotome Ab Interno Trabeculectomy

Cited by 3 publications

References 38 publications

Ripasudil in a model of pigmentary glaucoma

Ripasudil in a model of pigmentary glaucoma

Tissue-Engineered Anterior Segment Eye Cultures Maintain Intraocular Pressure

A Predictive Test of Outflow Enhancement by Ab Interno Trabeculectomy

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