Intranuclear Distribution of the Inducing Metal in Primary Rhabdomyosarcomata induced in the Rat by Nickel, Cobalt and Cadmium

Webb, M.; Heath, J. C.; Hopkins, T. R.

doi:10.1038/bjc.1972.37

Cited by 27 publications

(16 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since in the present work the nucleoli were isolated after treatment with DNase ( Fig. 1), the average RNA: DNA ratio (1-5 : 1) differed from that of the nucleolar preparations from primary nickel-induced rhabdomyosarcomata (RNA: DNA 0 33: 1; Webb et al, 1972). Also, in the fractionation of the C57S/IP nuclei, only about 60 % of the nuclear 63Ni2+ was recovered in the nucleolar and " nuclear sap + DNA " fractions, whilst 30-40 % was removed in the supernatant fraction, S4 (Fig.…”

Section: Resultsmentioning

confidence: 69%

See 1 more Smart Citation

Uptake of 63Ni2+ from its Complexes with Proteins and other Ligands by Mouse Dermal Fibroblasts in vitro

Webb¹,

Weinzierl²

1972

Br J Cancer

Self Cite

View full text Add to dashboard Cite

Summary.-A study has been made of the uptake of 63Ni2+ from its complexes with serum proteins and with the small molecules from a sterile autolysate of rat muscle by cells of the C57S/IP line of mouse dermal fibroblasts in vitro. Nickel from these complexes is incorporated intracellularly, the distribution being independent of the nature of the carrier. The cation is associated with all subcellular fractions, the largest amount being bound by the nuclei and the least by the microsomes. About half of the 63Ni2+ incorporated into the nuclei is located in the nucleoli.IT has been shown that the carcinogenic metals, cobalt, cadmium and nickel dissolve when incubated aseptically in horse serum and in homogenates of rat muscle with the formation of complexes of various biological ligands (Heath, Webb and Caffrey, 1969;Weinzierl and Webb, 1972). After solution in serum the dissolved cations are bound by both large (e.g. protein) and small (diffusible) molecules, whereas in muscle homogenates they are complexed almost entirely (e.g. 90 %) with compounds of low molecular weight. The products obtained on solution of nickel in a muscle homogenate in vitro are similar to those formed when implants of the metal dissolve in whole muscle in vivo, and thus it seems that these small molecular complexes may be the normal cation carriers that are involved in the transport of the cation during carcinogenesis. The cobalt serum complex obtained by dissolution of the metal in horse serum, however, also produces cytological changes in cultures of rat myoblasts similar to those seen in pre-malignant cells in vivo (Heath et al., 1969).To investigate whether carcinogenic metals, when bound by ligands of both high and low molecular weight, are incorporated by mammalian cells, a study has

show abstract

Section: Resultsmentioning

confidence: 69%

“…1) were resuspended in water or RSB for analysis. Nucleolar preparations were examined microscopically, methanol-fixed smears being stained by the methods given previously (Webb, Heath and Hopkins, 1972).…”

mentioning

confidence: 99%

Uptake of 63Ni2+ from its Complexes with Proteins and other Ligands by Mouse Dermal Fibroblasts in vitro

Webb¹,

Weinzierl²

1972

Br J Cancer

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cellular Ca* has been shown to be involved in a multitude of cellular regulation process ; Ca* plays a major role particularly in the regulation of cell proliferation (Swierenga et al 1980;Chafouleas et al 1984). Ca* was also found (Borle 1972), the second one is cytoplasmic exchangeable Nip consisting of several subpools, (mainly cell free anion) and binding to soluble ligand in the cytosol or binding to subcellular structure, and the third one is a pool of Nip sequestered in subcellular structure, presumably mitochondria, nucleus or endoplasmic reticulum as in the case of Cam (Borle 1973;Webb et al 1972). In the present study, the exchange of Nip between the incubation medium and the first, the second and the third compartment in the cells has been observed.…”

Section: Methodsmentioning

confidence: 99%

“…The pulmonary tissues with regional lymphnodes appear to be the preferential organs for the nickel retention (Waksvik et al 1984). In the lung of rats exposed to nickel, high concentration of nickel was found in the microsomal fraction (Sunderman 1964) and to be bound to RNA, protein (Sunderman and Sunderman 1963) and to nucleus (Webb et al 1972). However, the mechanisms of the accumulation of nickel into pulnomary cells are still not clear.…”

mentioning

confidence: 99%

Accumulation and efflux of nickel from cultured pneumocytes.

Saito

Menzel

1986

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

The accumulation and efflux of nickel from three kinds of cultured pneumocytes were studied. Nickel rapidly penetrated the cells examined. The concentration of nickel in the cells was related to the volum of cytoplasm and surface area of the cells. Some metabolic inhibitors (NaN3, NaCN, NaAsO2) reduced the accumulation of nickel by up to 74° . Extracellular calcium regulated the accumulation of nickel into the cells. CaC12 at 10 mM inhibited partially the accumulation of nickel, while nickel accumulation was enhanced in calcium free medium. From the efflux experiment, nickel was shown to be distributed to three different compartments in the cells, i.e., cell membrane, cytoplasm and intracellular constituents. Three rate constants were determined as k1=1.67-5.33, k2 =0.031-0.037 and k3 = 0.002-0.007 min-1. These results suggest that cell membrane is readily permeable to nickel and that saturable nickel-binding sites might exist in the pneumocytes examined,

show abstract

“…They have suggested that cobalt-protein complexes, absorbed on the surface of myoblasts, may enter the cell by endocytosis and that subsequent digestion of the carrier proteins by lysosomal proteinases leads to intracellular liberation and redistribution of cobalt. A high proportion of intracellular cobalt hasbeen found in the nuclei of muscle cells, more than half of this within nucleoli, where it would be well placed to exert an effect on DNA and RNA replication (16). Cobalt acetate has been shown to enhance viral transformation in embryonic cell culture (17).…”

Section: Comments and Evaluationmentioning

confidence: 99%

Role of cobalt, iron, lead, manganese, mercury, platinum, selenium, and titanium in carcinogenesis.

Kazantzis¹

1981

Environ Health Perspect

124

View full text Add to dashboard Cite

The possible carcinogenicity of cobalt, iron, lead, manganese, mercury, platinum, selenium, and titanium is reviewed, taking into account epidemiological data, the results of animal experimental studies, data on mutagenic effects and on other in vitro test systems. Of the great variety of occupations where exposure to one of these metals may occur, only haematite mining has been clearly shown to involve an increased human cancer risk. While the possibility that haematite might in some way act as a carcinogen has to be taken into consideration it is more likely that other carcinogens are responsible. Certain platinum coordination complexes are used in cancer chemotherapy, are mutagenic, and likely to be carcinogenic. Cobalt, its oxide and sulfide, certain lead salts, one organomanganese, and one organotitanium compound have been shown to have a limited carcinogenic effect in experimental animal studies, and except for titanium appear to be mutagenic. Certain mercury compounds are mutagenic but none have been shown to be carcinogenic. The presently available data are inadequate to assess the possible carcinogenicity of selenium compounds, but a few observations suggest that selenium may suppress the effect of other carcinogens administered to experimental animals and may even be associated with lower cancer mortality rates in man. Epidemiological observations are essential for the assessment of a human cancer risk, but the difficulty in collecting past exposure data in occupational groups and the complexity of multiple occupational exposures with changes over time, limits the usefulness of retrospective epidemiological studies.

show abstract

Intranuclear Distribution of the Inducing Metal in Primary Rhabdomyosarcomata induced in the Rat by Nickel, Cobalt and Cadmium

Cited by 27 publications

References 13 publications

Uptake of 63Ni2+ from its Complexes with Proteins and other Ligands by Mouse Dermal Fibroblasts in vitro

Uptake of 63Ni2+ from its Complexes with Proteins and other Ligands by Mouse Dermal Fibroblasts in vitro

Accumulation and efflux of nickel from cultured pneumocytes.

Role of cobalt, iron, lead, manganese, mercury, platinum, selenium, and titanium in carcinogenesis.

Contact Info

Product

Resources

About