Intranasal Inoculation of Mice with<i>Yersinia pseudotuberculosis</i>Causes a Lethal Lung Infection That Is Dependent on<i>Yersinia</i>Outer Proteins andPhoP

Fisher, Michael L.; Castillo, Cynthia; Mecsas, Joan

doi:10.1128/iai.01287-06

Cited by 41 publications

(45 citation statements)

References 88 publications

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“…S2), wild-type Y. pseudotuberculosis IP2666c was unable to trans-complement isogenic mutants lacking the Yersinia virulence plasmid (designated pYV in Y. pseudotuberculosis) or pCD1 − mutants of Y. pestis during pulmonary coinfection (Fig. 3A) (12). Wild-type Y. pseudotuberculosis IP32953, which causes a lung infection with nearly the same bacterial burden as Y. pestis, was also unable to transcomplement an isogenic pYV − strain (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Respiratory coinfection with wild-type Y. pseudotuberculosis is unable to rescue bacterial replication defects of individual Yop mutants (12). All pathogenic Yersinia harbor a nearly identical Ysc T3SS, which directly injects into host cells a number of cytotoxins or effector proteins (Yops) that inhibit bacterial phagocytosis and other innate immune processes (13).…”

Section: Resultsmentioning

confidence: 99%

“…Groups of four to five mice were inoculated subcutaneously in the front neck with 1,000 CFU of Y. pestis for both individual and coinfections. For both Y. pestis and Y. pseudotuberculosis, lacZ mutants (10,12) were designated as our "wild-type" strain, and blue/white screening was used to differentiate between coinoculated strains. Bacterial organ burdens were determined by plating bacteria on BHI or LB agar containing X-Gal for experiments involving multiple Y. pestis or Y. pseudotuberculosis strains.…”

Section: Methodsmentioning

confidence: 99%

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Pulmonary infection by Yersinia pestis rapidly establishes a permissive environment for microbial proliferation

Price

Jin

Goldman³

2012

Proc. Natl. Acad. Sci. U.S.A.

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Disease progression of primary pneumonic plague is biphasic, consisting of a preinflammatory and a proinflammatory phase. During the long preinflammatory phase, bacteria replicate to high levels, seemingly uninhibited by normal pulmonary defenses. In a coinfection model of pneumonic plague, it appears that Yersinia pestis quickly creates a localized, dominant anti-inflammatory state that allows for the survival and rapid growth of both itself and normally avirulent organisms. Yersinia pseudotuberculosis, the relatively recent progenitor of Y. pestis, shows no similar trans-complementation effect, which is unprecedented among other respiratory pathogens. We demonstrate that the effectors secreted by the Ysc type III secretion system are necessary but not sufficient to mediate this apparent immunosuppression. Even an unbiased negative selection screen using a vast pool of Y. pestis mutants revealed no selection against any known virulence genes, demonstrating the transformation of the lung from a highly restrictive to a generally permissive environment during the preinflammatory phase of pneumonic plague.inflammation | pathogenesis

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Pulmonary infection by Yersinia pestis rapidly establishes a permissive environment for microbial proliferation

Price

Jin

Goldman³

2012

Proc. Natl. Acad. Sci. U.S.A.

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“…For example, Yersinia pseudotuberculosis is a gut pathogen transmitted by the fecal-oral route, but it has the capacity to be lethal when it (rarely) invades the lungs or blood (176). The plague bacterium, Yersinia pestis, which is derived from Y. pseudotuberculosis, inherited its ancestor's capacity for systemic infection, but it evolved to reside primarily in the hosts' blood through transmission by fleas (177).…”

Section: Resource Differences Among Pathogensmentioning

confidence: 99%

Transmission in the Origins of Bacterial Diversity, From Ecotypes to Phyla

Cohan

2017

Microbiol Spectr

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Any two lineages, no matter how distant they are now, began their divergence as one population splitting into two lineages that could coexist indefinitely. The rate of origin of higher-level taxa is therefore the product of the rate of speciation times the probability that two new species coexist long enough to reach a particular level of divergence. Here I have explored these two parameters of disparification in bacteria. Owing to low recombination rates, sexual isolation is not a necessary milestone of bacterial speciation. Rather, irreversible and indefinite divergence begins with ecological diversification, that is, transmission of a bacterial lineage to a new ecological niche, possibly to a new microhabitat but at least to new resources. Several algorithms use sequence data from a taxon of focus to identify phylogenetic groups likely to bear the dynamic properties of species. Identifying these newly divergent lineages allows us to characterize the genetic bases of speciation, as well as the ecological dimensions upon which new species diverge. Speciation appears to be least frequent when a given lineage has few new resources it can adopt, as exemplified by photoautotrophs, C1 heterotrophs, and obligately intracellular pathogens; speciation is likely most rapid for generalist heterotrophs. The genetic basis of ecological divergence may determine whether ecological divergence is irreversible and whether lineages will diverge indefinitely into the future. Long-term coexistence is most likely when newly divergent lineages utilize at least some resources not shared with the other and when the resources themselves will coexist into the remote future.

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“…8,18 Among the Yops translocated by the TTSS, YopH and YopB are important for lung colonization and dissemination. 8,19 The chromosomally encoded Inv product enables the organism to invade Peyer's patch M-cells. 9,19 Genotyping on the basis of these virulence-associated genes has been used to predict the phenotypes of field isolates.…”

Section: -13mentioning

confidence: 99%

Fatal Yersiniosis in Farmed Deer Caused by Yersinia Pseudotuberculosis Serotype O:3 Encoding a Mannosyltransferase-Like Protein WbyK

Zhang

Liu

et al. 2008

J VET Diagn Invest

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Abstract. Sudden death of 9 deer occurred in a large enclosed deer farm with approximately 400 heads of cervids. Fatal yersiniosis was diagnosed in 2 deer that were submitted for laboratory diagnosis. Histopathologically, the disease was characterized by multifocal pulmonary hemorrhage and mild interstitial pneumonia, marked diffuse cholangiohepatitis, minimal myocarditis with mild myocardial degeneration, and mild multifocal suppurative cystic colitis. Yersinia pseudotuberculosis was isolated from the lungs and colon of the affected animals. The isolates were PCR-positive for genes virF, inv, yopB, and yopH, which are essential for invasion and colonization of host intestine and lung. The isolates reacted with polyclonal antibodies against serotype O:3 antigen. The O-genotyping patterns of the isolates were identical with each other, but different from those of the 21 O-genotypes (or serotypes) reported previously. In addition to the O-antigen genes possessed by classical serotype O:3, a gene (wbyK ) encoding a mannosyltransferase-like protein was detected in these isolates. The wbyK gene of the isolates showed 94% of DNA sequence homology with the wbyK gene harbored by Y. pseudotuberculosis O:1b. On the basis of pathology, bacteriology, and serology, the authors concluded that the acute deaths of these deer were caused by Y. pseudotuberculosis infection. Molecular characterization of the isolate revealed a genetic heterogeneity in the O-antigen gene cluster of Y. pseudotuberculosis serotype O:3.

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Intranasal Inoculation of Mice withYersinia pseudotuberculosisCauses a Lethal Lung Infection That Is Dependent onYersiniaOuter Proteins andPhoP

Cited by 41 publications

References 88 publications

Pulmonary infection by Yersinia pestis rapidly establishes a permissive environment for microbial proliferation

Pulmonary infection by Yersinia pestis rapidly establishes a permissive environment for microbial proliferation

Transmission in the Origins of Bacterial Diversity, From Ecotypes to Phyla

Fatal Yersiniosis in Farmed Deer Caused by Yersinia Pseudotuberculosis Serotype O:3 Encoding a Mannosyltransferase-Like Protein WbyK

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