Intranasal infection with Bordetella bronchiseptica in gnotobiotic piglets

Brassinne, M de la; Dewaele, A.; Gouffaux, M.

doi:10.1016/s0034-5288(18)33450-7

Cited by 24 publications

(9 citation statements)

References 3 publications

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“…Swine atrophic rhinitis (AR) is a disease characterized by severe necrosis of epithelia of the upper respiratory tract and by deformity and reduction both in volume and size of the nasal turbinates and snouts (5,8,18,19,23,30,36,39). B. bronchiseptica causes marked loss of cilia accompanied by the characteristic morphological changes of the nasal mucosa, if the organisms at phase I (24) are intranasally inoculated into gnotobiotic or specific pathogen-free neonatal pigs (5,8,18,19,20,30,36,39). Injection of pure cultures of B. bronchiseptica also results in severe nasal turbinate atrophy; therefore, B. bronchiseptica has been considered as a primary causative agent for swine AR.…”

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confidence: 99%

Properties of dermonecrotic toxin prepared from sonic extracts Bordetella bronchiseptica

Kume¹,

Nakai²,

Samejima³

et al. 1986

Infect Immun

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A toxin with dermonecrotic activity (DNT) was purified from sonic extracts of Bordetella bronchiseptica L3 of pig origin at phase I by chromatographic and electrophoretic methods. The purification procedure was one developed for obtaining the Pasteurella multocida DNT from sonic extracts with some modifications. Dermonecrotizing activity of B. bronchiseptica-purified DNT was increased by 600-fold compared with that of the crude extract, and the average yield was about 3%. The toxin was homogeneous, as determined by Ouchterlony double immunodiffusion, crossed immunoelectrophoresis, and disk isoelectric focusing in polyacrylamide gels. The toxin gave a single band on polyacrylamide disk gel electrophoresis (PAGE) and sodium dodecyl sulfate-SDS PAGE. The molecular weight of the toxin was ca. 190,000 + 5,000, as determined by SDS-PAGE. The isoelectric point of the toxin was ca. 6.5 to 6.6. The minimal necrotizing dose of the toxin for guinea pigs was about 2 ng of protein per 0.1 ml, the 50% lethal dose per mouse was about 0.3 ,ug, and the minimal cytotoxic dose for embryonic bovine lung cells was about 2 ng/ml. The toxin was heat labile and sensitive to inactivation by trypsin, Formalin, and glutaraldehyde. The mildly trypsinized B. bronchiseptica DNT preparation dissociated into two polypeptide chains, with molecular weights of ca. 75,000 ± 4,000 (fragment 1) and ca. 118,000 ± 5,000 (fragment 2), after treatment with dithiothreitol-SDS or urea. Upon removal of dithiothreitol and urea from the dissociated DNT preparation, the fragments reassociated, and the DNT that was formed was indistinguishable from the native toxin.

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mentioning

confidence: 99%

Properties of dermonecrotic toxin prepared from sonic extracts Bordetella bronchiseptica

Kume¹,

Nakai²,

Samejima³

et al. 1986

Infect Immun

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“…It is important as a respiratory pathogen in pigs, playing a primary aetiological role in pneumonia (Duncan, Ramsey & Switzer, 1966), and possibly also in the development of atrophic rhinitis (Brassinne, Dewaele & Gouffaux, 1976). In dogs the trachea and bronchi are mainly affected, resulting in the tracheobronchitis known as kennel cough (Thompson, McCandlish & Wright, 1976).…”

Section: Bordetella Bronchisepticamentioning

confidence: 99%

An outbreak of Bordetella bronchiseptica pneumonia in a colony of common marmosets (Callithrix jacchus)

Baskerville¹,

Wood²,

Baskerville

1983

Lab Anim

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An outbreak of Bordetella bronchiseptica pneumonia occurred in a breeding colony of common marmosets (Callithrix jacchus). 16 animals, all except one under 12 months of age, died suddenly. Extensive lesions of pneumonia and pleurisy were found at necropsy and B. bronchiseptica was isolated from the nasopharynx, trachea and lungs. Older animals had only a mild rhinitis. Colonization of the nasal mucosa occurred in 71 of 156 marmosets.

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“…Severe porcine atrophic rhinitis is characterized by reduction in the size of the nasal turbinates accompanied by brachygnathia superior or twisting of the snout (5,13,24,25,28,32). Atrophic rhinitis is a multifactorial disease involving herd management, environment, heredity, and infectious agents, including Bordetella bronchiseptica and Pasteurella multocida.…”

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confidence: 99%

Survival of toxigenic Pasteurella multocida in aerosols and aqueous liquids

Thomson

Chanter

Wathes

1992

Appl Environ Microbiol

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The survival of toxigenic Pasteurella multocida in air and liquids was studied to identify possible risk factors in the etiology of atrophic rhinitis. In aerosols, at low relative humidity (28%), the viability of toxigenic P. multocida 5 min after aerosolization was at least 22% of its initial value. Viability at low relative humidity declined to 8% after 45 min. Viability at high relative humidity (79%) was 69% after 5 min and declined to 2% after 45 min. Survival of toxigenic P. multocida in liquids depended on storage and constituents in the liquid. Toxigenic P. multocida became nonculturable 1 to 14 days after inoculation in water and artificial seawater, depending on the storage temperature. Toxigenic P. multocida stored at 37°C could be detected for up to 6 days in pig slurry and more than 36 days in Bacto Tryptose broth and nasal lavages. However, in Bacto Tryptose broth and nasal lavages stored at 4°C, P. multocida was detected for up to 14 days whereas at 15 and 37°C it was detected for more than 49 days. These results suggest that aerosols and fomites can play a role in the transmission of atrophic rhinitis.

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Intranasal infection with Bordetella bronchiseptica in gnotobiotic piglets

Cited by 24 publications

References 3 publications

Properties of dermonecrotic toxin prepared from sonic extracts Bordetella bronchiseptica

Properties of dermonecrotic toxin prepared from sonic extracts Bordetella bronchiseptica

An outbreak of Bordetella bronchiseptica pneumonia in a colony of common marmosets (Callithrix jacchus)

Survival of toxigenic Pasteurella multocida in aerosols and aqueous liquids

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