Intranasal immunization with C. muridarum major outer membrane protein (MOMP) and cholera toxin elicits local production of neutralising IgA in the prostate

Hickey, Danica K.; Jones, Russell C.; Bao, Shisan; Blake, Anita E.; Skelding, Kathryn A.; Berry, Linda J.; Beagley, Kenneth W.

doi:10.1016/j.vaccine.2004.04.021

Cited by 30 publications

(17 citation statements)

References 46 publications

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“…More importantly, to definitively confirm expression of Ig molecules in spermatogenic cells, expression of rearranged Ig gene transcripts should be identified in these cells, because extracellular IgG could be transferred into epithelial cells through FcRn (neonatal Fc receptor) or pIgRs (polymeric immunoglobulin receptor) expressed in the reproductive ducts including the ductuli efferentes (where most IgG was reabsorbed) and the distal caput epididymidis (Gurevich et al 2003;Hickey et al 2004;Knee et al 2005). By ISH and RT-PCR, we also found Ig g1 and k chain transcripts existed in testicular spermatogenic cells and epididymal epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

Expression of Immunoglobulin Gene With Classical V-(D)-J Rearrangement in Mouse Testis and Epididymis

Huang

Zhang

et al. 2008

J Histochem Cytochem.

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Immunoglobulin (Ig) molecules have thus far been found only to be produced by differentiated B lymphocytes. As we know, immune privilege in the normal adult mouse testis exists to make these privileged sites generally free of patrolling immune cells and Igs. However, in this study, the expression of Ig in normal adult mouse testis and epididymis was detected. First, by IHC, a strong pattern of Ig expression was detected in the spermatogenic cells of adult mouse testis at different development stages. Second, by Western blot analysis, different strong bands of Igs in mouse testicular spermatogenic cells and epididymal epithelial cell extract were recognized using specific antibodies against IgG. More importantly, by ISH and cell sorting-related RT-PCR, rearranged Ig gamma chain and kappa chain transcripts were expressed in testicular spermatogenic cells and epididymal epithelial cells. These results suggested that Ig in testis and epididymis was mainly produced by adult mouse testicular spermatogenic cells and epididymal epithelial cells.

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Section: Discussionmentioning

confidence: 99%

Expression of Immunoglobulin Gene With Classical V-(D)-J Rearrangement in Mouse Testis and Epididymis

Huang

Zhang

et al. 2008

J Histochem Cytochem.

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“…Indeed, there is a need to examine the entry of other classes of antibody into the lumen of the reproductive ducts. For example, IgA and IgM as well as IgG have been found in human and boar semen [54,55], and in humans, prostatic fluid contains IgG [56] and the prostate gland secretes IgA [57][58][59][60]. It is possible that sufficient IgA may be secreted into accessory gland fluids for this purpose so that it is worthwhile examining mucosal as well as systemic immunization to raise the immune response.…”

Section: Discussionmentioning

confidence: 99%

Transport of IgG across the Blood-Luminal Barrier of the Male Reproductive Tract of the Rat and the Effect of Estradiol Administration on Reabsorption of Fluid and IgG by the Epididymal Ducts1

Knee

Hickey

Beagley

et al. 2005

Biology of Reproduction

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In rats immunized systemically with tetanus toxoid the concentration of specific anti-tetanus-toxoid-specific IgG in fluid from the rete testis and cauda epididymidis were respectively 0.6% and 1.4% the concentration in blood serum. The extratesticular duct system reabsorbed 97% of the IgG and 99% of the fluid leaving the rete, but estradiol administration affected the site of reabsorption. In untreated rats, the ductuli efferentes reabsorbed 94% of the IgG and 96% of the fluid leaving the rete, whereas estradiol-treated rats reabsorbed 83% of the IgG and 86% of the fluid, and the ductus epididymidis fully compensated for these different effects of estradiol on the ductuli efferentes. The concentrations of IgG in secretions of the seminal vesicles and prostate gland were lower (0.1% and 0.3% respectively of the titers in blood serum) than in fluids from the extratesticular ducts, and were not affected by the administration of estradiol. RT-PCR showed that Fcgrt (neonatal Fc receptor, also known as FcRn) is expressed in the reproductive ducts, where IgG is probably transported across epithelium, being particularly strong in the ductuli efferentes (where most IgG was reabsorbed) and distal caput epididymidis. It is concluded that IgG enters the rete testis and is concentrated only 2.5-fold along the extratesticular duct system, unlike spermatozoa, which are concentrated 95-fold. Further, the ductus epididymidis can recognize and compensate for changes in function of the ductuli efferentes.

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“…Secretory IgA antibodies which are secreted by B cells in the mucosa are resistant to proteolytic cleavage in the protease rich milieu of genital tract and other mucosa sites [3,7,22]. Secretory IgA antibodies have also been shown to protect from chlamydial infections in mice [7,[24][25][26][27] and humans [28]. Induction of mucosal IgA antibodies that protect from chlamydial infections is therefore of prime significance in its vaccine development regimen as recently demonstrated [14].…”

Section: Discussionmentioning

confidence: 99%

A Peptide Containing T-Cell Epitopes of <i>Chlamydia trachomatis</i> Recombinant MOMP Induces Systemic and Mucosal Antibody Responses in Mice

Taha¹,

Singh²,

Hulett³

et al. 2011

WJV

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Intranasal immunization with C. muridarum major outer membrane protein (MOMP) and cholera toxin elicits local production of neutralising IgA in the prostate

Cited by 30 publications

References 46 publications

Expression of Immunoglobulin Gene With Classical V-(D)-J Rearrangement in Mouse Testis and Epididymis

Expression of Immunoglobulin Gene With Classical V-(D)-J Rearrangement in Mouse Testis and Epididymis

Transport of IgG across the Blood-Luminal Barrier of the Male Reproductive Tract of the Rat and the Effect of Estradiol Administration on Reabsorption of Fluid and IgG by the Epididymal Ducts1

A Peptide Containing T-Cell Epitopes of <i>Chlamydia trachomatis</i> Recombinant MOMP Induces Systemic and Mucosal Antibody Responses in Mice

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Intranasal immunization with C. muridarum major outer membrane protein (MOMP) and cholera toxin elicits local production of neutralising IgA in the prostate

Cited by 30 publications

References 46 publications

Expression of Immunoglobulin Gene With Classical V-(D)-J Rearrangement in Mouse Testis and Epididymis

Expression of Immunoglobulin Gene With Classical V-(D)-J Rearrangement in Mouse Testis and Epididymis

Transport of IgG across the Blood-Luminal Barrier of the Male Reproductive Tract of the Rat and the Effect of Estradiol Administration on Reabsorption of Fluid and IgG by the Epididymal Ducts1

A Peptide Containing T-Cell Epitopes of &lt;i&gt;Chlamydia trachomatis&lt;/i&gt; Recombinant MOMP Induces Systemic and Mucosal Antibody Responses in Mice

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A Peptide Containing T-Cell Epitopes of <i>Chlamydia trachomatis</i> Recombinant MOMP Induces Systemic and Mucosal Antibody Responses in Mice