Intranasal Immunization of Ferrets with Commercial Trivalent Influenza Vaccines Formulated in a Nanoemulsion-Based Adjuvant

Hamouda, Tarek; Sutcliffe, Joyce A.; Ciotti, Susan; Baker, James R.

doi:10.1128/cvi.00035-11

Cited by 49 publications

(33 citation statements)

References 30 publications

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“…In 2009, a number of investigators used the ferret model to evaluate the replication and pathogenesis of the newly emerged H1N1 pandemic (H1N1pdm) virus (10,(17)(18)(19). Although the viruses and doses administered were similar, different investigators reported a range of clinical disease outcomes, from asymptomatic infection to severe weight loss, ocular and nasal discharge, sneezing, and lethargy (14,17,18,20,21). Our review of the studies revealed that a wide range of inoculum volumes (0.2 to 3 ml) was used to experimentally infect the ferrets; we sought to determine whether the variations in disease outcome were the result of the volume of inoculum administered.…”

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confidence: 99%

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Severity of Clinical Disease and Pathology in Ferrets Experimentally Infected with Influenza Viruses Is Influenced by Inoculum Volume

Moore

Lamirande

Paskel

et al. 2014

J Virol

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Ferrets are a valuable model for influenza virus pathogenesis, virus transmission, and antiviral therapy studies. However, the contributions of the volume of inoculum administered and the ferret's respiratory tract anatomy to disease outcome have not been explored. We noted variations in clinical disease outcomes and the volume of inoculum administered and investigated these differences by administering two influenza viruses (A/California/07/2009 [H1N1 pandemic] and A/Minnesota/11/2010 [H3N2 variant]) to ferrets intranasally at a dose of 10 6 50% tissue culture infective doses in a range of inoculum volumes (0.2, 0.5, or 1.0 ml) and followed viral replication, clinical disease, and pathology over 6 days. Clinical illness and respiratory tract pathology were the most severe and most consistent when the viruses were administered in a volume of 1.0 ml. Using a modified micro-computed tomography imaging method and examining gross specimens, we found that the right main-stem bronchus was consistently larger in diameter than the left main-stem bronchus, though the latter was longer and straighter. These anatomic features likely influence the distribution of the inoculum in the lower respiratory tract. A 1.0-ml volume of inoculum is optimal for delivery of virus to the lower respiratory tract of ferrets, particularly when evaluation of clinical disease is desired. Furthermore, we highlight important anatomical features of the ferret lung that influence the kinetics of viral replication, clinical disease severity, and lung pathology. IMPORTANCEFerrets are a valuable model for influenza virus pathogenesis, virus transmission, and antiviral therapy studies. Clinical disease in ferrets is an important parameter in evaluating the virulence of novel influenza viruses, and findings are extrapolated to virulence in humans. Therefore, it is highly desirable that the data from different laboratories be accurate and reproducible. We have found that, even when the same virus was administered at similar doses, different investigators reported a range of clinical disease outcomes, from asymptomatic infection to severe weight loss, ocular and nasal discharge, sneezing, and lethargy. We found that a wide range of inoculum volumes was used to experimentally infect ferrets, and we sought to determine whether the variations in disease outcome were the result of the volume of inoculum administered. These data highlight some less explored features of the model, methods of experimental infection, and clinical disease outcomes in a research setting.

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confidence: 99%

“…In addition, ferrets are relatively easy to maintain and handle in a research setting and can be naturally infected with most human influenza viruses (2). Use of the ferret in influenza virus research has expanded to include virus transmission (7)(8)(9)(10)(11)(12), vaccine efficacy (13,14), and antiviral therapeutics studies (15,16).…”

mentioning

confidence: 99%

Severity of Clinical Disease and Pathology in Ferrets Experimentally Infected with Influenza Viruses Is Influenced by Inoculum Volume

Moore

Lamirande

Paskel

et al. 2014

J Virol

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“…A good demonstration that adjuvantation can significantly reduce the amount of antigen required is provided by the work of Baras et al (2008), who have shown that intramuscular immunisation of ferrets with 3.8 µg of haemagglutination (HA) from inactivated split H5N1 virus in an oil-in-water emulsion-based adjuvant was able to protect the ferrets against a lethal challenge with a heterologous H5N1 virus; the amount of HA used in this experiment was much smaller than the amount required for normal vaccination (15 µg). The results obtained by Hamouda et al (2011) also demonstrated that intranasal immunisation of ferrets with the virus antigen in a soybean oil-inwater nanoemulsion induced a HA inhibition titre approximately 90-fold higher than that resulting from the standard intramuscular nonadjuvanted commercial influenza vaccine dose. Despite the fact that some oil-inwater-based adjuvants in influenza vaccines can be well tolerated by humans (Leroux-Roels et al 2007), alum is the adjuvant used predominantly in human vaccines; however, there is still the need to identify compounds with better adjuvant properties.…”

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confidence: 56%

VaxcineTM: an oil-based adjuvant for influenza vaccines

Alves

New

Andrade

et al. 2011

Mem. Inst. Oswaldo Cruz

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Vaccination is the method of choice for the prevention of influenza infection. However, the quantity of the antigen available, especially in the case of pandemics, often fails to meet the global demand. However, improved adjuvants can overcome this problem. Preliminary results obtained in this study revealed that one year after a single subcutaneous immunisation with influenza A H3N2 virus in an oil-based carrier, VaxcineTM, outbreed mice produced a high immunoglobulin G response that lasted for up to one year and exhibited less variation in titre compared with the response of the control group treated with alum. The haemagglutination-inhibition titres induced by VaxcineTM were also higher than those generated by alum. These data indicate that VaxcineTM is a good adjuvant candidate for seasonal influenza vaccines

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“…Hamouda et al [29,30] developed an influenza vaccine adjuvant oil-in-water nanoemulsion-based adjuvant W805EC, which immunized ferrets or mice by nasal cavity to produce safe and specific cellular and humoral immune responses. The hemagglutination inhibitory antibody produced by the nanoemulsionbased adjuvant vaccine was 19 to 90 times that of without the adjuvant vaccine.…”

Section: Construction and Immunological Evaluation Of Organic Nanocommentioning

confidence: 99%

Construction and Immunological Evaluation of Immunostimulatory Nanocomposites with Vaccine Potential

Zhong¹,

Zhuang²

2017

J Infect Dis Ther

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For a long time, the most important means of controlling infectious diseases is prevention, and vaccination is considered the most effective measures, so the preparation and improvement of the vaccine is not only supported by the medical profession, it is the concern of the broad masses of the people. A number of biomolecules have been used as antigens to construct novel vaccine preparations, including Virus-like particles (VLPs) or bacteria, dendritic cells, nucleic acids and peptides. Immunostimulatory nanocomposites are based on the above molecules as vectors, encapsulating immune adjuvants, and nanoparticles for the construction of novel vaccines. Compared to traditional vaccines, adjuvants, nanoparticles alone, the efficacy of immunostimulatory nanocomposites as potential vaccines shows increased CD4 + , CD8+ T cell count, secretion of cytokine interferon-gamma and IL-4 functions also significantly enhanced, in addition, the use of nanoparticles enhances the Immune irritation of adjuvants, VLPs and other molecules on humoral and cellular immune responses. Thus, immunostimulatory nanocomposites may be potential vaccines for future prevention and treatment applications.

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Intranasal Immunization of Ferrets with Commercial Trivalent Influenza Vaccines Formulated in a Nanoemulsion-Based Adjuvant

Cited by 49 publications

References 30 publications

Severity of Clinical Disease and Pathology in Ferrets Experimentally Infected with Influenza Viruses Is Influenced by Inoculum Volume

Severity of Clinical Disease and Pathology in Ferrets Experimentally Infected with Influenza Viruses Is Influenced by Inoculum Volume

VaxcineTM: an oil-based adjuvant for influenza vaccines

Construction and Immunological Evaluation of Immunostimulatory Nanocomposites with Vaccine Potential

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