Intranasal challenge with increasing ovalbumin doses differently affects airway hyperresponsiveness and inflammatory cell accumulation in mouse model of asthma

Bošnjak, Berislav; Tkalčević, Vanesa Ivetić; Đurić, Koraljka; Belamarić, Daniela; Čužić, Snježana; Ferenčić, Željko; Brajša, Karmen; Glojnarić, Ines; Antolović, Roberto; Hrvačić, Boška

doi:10.1007/s00011-009-0046-2

Cited by 3 publications

(1 citation statement)

References 29 publications

(50 reference statements)

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“…When we increased the dose to 1 µg (∼300 pollen grains), 10 µg (∼3,000 pollen grains) and 100 µg (∼30,000 pollen grains), the number of airway inflammatory cells increased, most notably the percentage of eosinophils and neutrophils, with 100 µg leading to the most severe disease. Thus, titrated doses of RWP caused increasing severity of acute airway and lung disease, as previously shown in mice and patients (10,11,13,14,40,41). Because endotoxin is present in the samples, increasing pollen dose also increases the endotoxin content and together they could induce the more intense, mixed inflammatory response that we observed.…”

Section: Discussionsupporting

confidence: 77%

Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation

et al. 2022

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Common ragweed (Ambrosia artemisiifolia) is an invasive plant with allergenic pollen. Due to environmental changes, ragweed pollen (RWP) airborne concentrations are predicted to quadruple in Europe by 2050 and more than double allergic sensitization of Europeans by 2060. We developed an experimental RWP model of allergy in BALB/c mice to evaluate how the number of RWP and how RWP collected from different geographical environments influence disease. We administered RWP six times over 3 weeks intranasally to the mice and then evaluated disease parameters 72 h later or allowed the mice to recover for at least 90 days before rechallenging them with RWP to elicit a disease relapse. Doses over 300 pollen grains induced lung eosinophilia. Higher doses of 3,000 and 30,000 pollen grains increased both eosinophils and neutrophils and induced disease relapses. RWP harvested from diverse geographical regions induced a spectrum of allergic lung disease from mild inflammation to moderate eosinophilic and severe mixed eosinophilic-neutrophilic lung infiltrates. After a recovery period, mice rechallenged with pollen developed a robust disease relapse. We found no correlation between Amb a 1 content, the major immunodominant allergen, endotoxin content, or RWP structure with disease severity. These results demonstrate that there is an environmental impact on RWP with clinical consequences that may underlie the increasing sensitization rates and the severity of pollen-induced disease exacerbation in patients. The multitude of diverse environmental factors governing distinctive patterns of disease induced by RWP remains unclear. Further studies are necessary to elucidate how the environment influences the complex interaction between RWP and human health.

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Section: Discussionsupporting

confidence: 77%

Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation

et al. 2022

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Establishment of a mouse model with all four clinical features of eosinophilic bronchitis

Chen

Peng

et al. 2020

Sci Rep

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Eosinophilic bronchitis (EB) is a clinical disease characterized by chronic cough, airway eosinophil infiltration, and responsive to steroid therapy but with the absence of airway hyperreactivity (AHR). This study established an EB mouse model with all the above features. First, 42 mice were divided into 7 groups to investigate the optimal time interval between cough and AHR detections. Afterward, 28 mice were divided into the asthma, EB, normal saline (NS), and dexamethasone (DXM) groups. Mice were challenged using nasal drops of 200 µg ovalbumin (OVA), 10 µg OVA, NS, or intraperitoneal injections of 5 mg/kg of DXM one hour prior to 10 µg OVA challenge. Airway reactivity was measured 6 h after cough was observed. The frequency of coughs in the asthma and EB groups increased significantly compared to mice in the NS group. After DXM administration, frequency of coughs was significantly decreased compared to mice in the asthma and EB groups. Lung resistance in the asthma group was significantly higher compared to mice in the NS, EB, and DXM groups. Obvious airway eosinophilic inflammation in BALF and lung tissues were observed in the asthma and EB groups, while DXM administration could attenuate airway inflammatory infiltration. In summary, we developed a mouse EB model with all four clinical features of EB by the administration of 10 µg OVA nasal drops.

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Dual Role of Interleukin-23 in Epicutaneously-Sensitized Asthma in Mice

Masaki

Suzuki

Kagawa

et al. 2014

Allergology International

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Intranasal challenge with increasing ovalbumin doses differently affects airway hyperresponsiveness and inflammatory cell accumulation in mouse model of asthma

Cited by 3 publications

References 29 publications

Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation

Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation

Establishment of a mouse model with all four clinical features of eosinophilic bronchitis

Dual Role of Interleukin-23 in Epicutaneously-Sensitized Asthma in Mice

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