Intranasal Administration of Recombinant<i>Neisseria gonorrhoeae</i>Transferrin Binding Proteins A and B Conjugated to the Cholera Toxin B Subunit Induces Systemic and Vaginal Antibodies in Mice

Price, Gregory A.; Russell, Michael W.; Cornelissen, Cynthia Nau

doi:10.1128/iai.73.7.3945-3953.2005

Cited by 58 publications

(66 citation statements)

References 49 publications

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“…Furthermore, we demonstrated bactericidal activity of immune sera from mice immunized with these Tbp-Ctb chemical conjugates [19]. One of the drawbacks to using the chemical conjugation of two proteins however is the heterologous nature of the vaccine preparation due to differential cross-linking of two proteins.…”

Section: Introductionmentioning

confidence: 93%

“…We demonstrated previously that intranasal immunization with the gonococcal transferrinbinding proteins chemically conjugated to the cholera toxin B subunit (Ctb) induced systemic and vaginal antibodies against both TbpA and TbpB [19]. Furthermore, we demonstrated bactericidal activity of immune sera from mice immunized with these Tbp-Ctb chemical conjugates [19].…”

Section: Introductionmentioning

confidence: 94%

“…Purified chimeras were bound to G M1 ganglioside in an ELISA format and complexes with binding competent Ctb pentamers were confirmed to contain NB and L2 with specific antibodies [19].…”

Section: G M1 Ganglioside Elisamentioning

confidence: 99%

“…All groups received two IP boosts 10 days apart. Sera and vaginal washes were obtained as described previously [19], and collected on days 0, 18, 28 (secretions only), 35, 63, and 82.…”

Section: Mouse Immunizations and Sample Collectionmentioning

confidence: 99%

“…Because L2 is relatively small in size, and from a protein that is not especially immunogenic [19], we constructed a double genetic chimera with this peptide. The strategy we employed expressed NB and L2 together in anticipation that the larger NB would be more immunogenic and could augment antibody responses to L2.…”

Section: Introductionmentioning

confidence: 99%

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Gonococcal transferrin binding protein chimeras induce bactericidal and growth inhibitory antibodies in mice

Price

Masri

Hollander

et al. 2007

Vaccine

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We have previously demonstrated the full-length gonococcal transferrin binding proteins (TbpA and TbpB) to be promising antigens in the development of a protective vaccine against Neisseria gonorrhoeae. In the current study we employed a genetic chimera approach fusing domains from TbpA and TbpB to the A2 domain of cholera toxin, which naturally binds in a non-covalent fashion to the B subunit of cholera toxin during assembly. For one construct, the N-terminal half of TbpB (NB) was fused to the A2 subunit of cholera toxin. In a second construct, the loop 2 region (L2) of TbpA was genetically fused between the NB domain and the A2 domain, generating a double chimera. Both chimeras were immunogenic and induced serum bactericidal and vaginal growthinhibiting antibodies. This study highlights the potential of using protective epitopes instead of fulllength proteins in the development of an efficacious gonococcal vaccine.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 94%

“…Purified chimeras were bound to G M1 ganglioside in an ELISA format and complexes with binding competent Ctb pentamers were confirmed to contain NB and L2 with specific antibodies [19].…”

Section: G M1 Ganglioside Elisamentioning

confidence: 99%

“…All groups received two IP boosts 10 days apart. Sera and vaginal washes were obtained as described previously [19], and collected on days 0, 18, 28 (secretions only), 35, 63, and 82.…”

Section: Mouse Immunizations and Sample Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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