Intramuscular DNA Vaccination of Juvenile Carp against Spring Viremia of Carp Virus Induces Full Protection and Establishes a Virus-Specific B and T Cell Response

Embregts, Carmen W.E.; Rigaudeau, Dimitri; Veselý, Tomáš; Pokorová, Dagmar; Lorenzen, Niels; Petit, Jules; Houel, Armel; Dauber, Malte; Schütze, Heike; Boudinot, Pierre; Wiegertjes, G.F.; Forlenza, Maria

doi:10.3389/fimmu.2017.01340

Cited by 39 publications

(23 citation statements)

References 72 publications

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“…A possible explanation for this observation may be that the expression of the VAA protein increases the percentage of sIgM + lymphocytes. Indeed, similar results have been noted in previous studies demonstrating the induction of sIgM + B lymphocytes following intramuscular or oral immunization with DNA plasmids (13,42,70). Taken together, these results suggest that the intramuscularly administrated pVAA vaccine induced a humoral immune response in flounder.…”

Section: Discussionsupporting

confidence: 90%

“…Once the protein has been synthesized, it is released from the myocytes either as a secreted protein or due to cell damage/apoptosis, upon which it is recognized and presented by antigen presenting cells (such as macrophages, dendritic cells, and B cells), resulting in the downstream activation of helper and cytotoxic T-cells (39,40). Expression of transfected viral proteins has been observed by many researchers in myocytes along with the infiltration of lymphocytes and macrophages around the injection site (41)(42)(43). In the present study, specific immunofluorescence around the injection site was observed in pVAA immunized fish.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Xing

Tang

et al. 2019

Front. Immunol.

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Vibrio anguillarum is a pathogenic bacterium that infects flounder resulting in significant losses in the aquaculture industry. The VAA protein previously identified in flounder is associated with a role in immune protection within these fish. In the present study, a recombinant DNA plasmid encoding the VAA gene of V. anguillarum was constructed and its potential as a DNA vaccine, to prevent the infection of V. anguillarum in flounder fish, investigated. We verified the expression of the VAA protein both in vitro in cell lines and in vivo in flounder fish. The protective effects of pcDNA3.1-VAA (pVAA) were analyzed by determination of the percentage of sIgM+, CD4-1+, CD4-2+, CD8β+ lymphocytes, and the production of VAA-specific antibodies in flounder following their immunization with the DNA vaccine. Histopathological changes in immune related tissues, bacterial load, and relative percentage survival rates of flounder post-challenge with V. anguillarum, were all investigated to assess the efficacy of the pVAA DNA vaccine candidate. Fish intramuscularly immunized with pVAA showed a significant increase in CD4-1+, CD4-2+, and CD8β+ T lymphocytes at days 9, 11, and 14 post-vaccination, reaching peak T-cell levels at days 11 or 14 post-immunization. The percentage of sIgM+ lymphocytes reached peak levels at weeks 4–5 post-immunization. Specific anti-V. anguillarum or anti-rVAA antibodies were induced in inoculated fish at days 28–35 post-immunization. The liver of vaccinated flounder exhibited only slight histopathological changes compared with a significant pathology observed in control immunized fish. Additionally, a lower bacterial burden in the liver, spleen, and kidney were observed in pVAA protected fish in response to bacterial challenge, compared with pcDNA3.1 vector control injected fish. Moreover, the pVAA vaccine confers a relative percentage survival of 50.00% following V. anguillarum infection. In summary, this is the first study indicating an initial induction of the T lymphocyte response, followed by B lymphocyte induction of specific antibodies as a result of DNA immunization of flounder. This signifies the important potential of pVAA as a DNA vaccine candidate for the control of V. anguillarum infection.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Xing

Tang

et al. 2019

Front. Immunol.

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“…For most rhabdovirus, the surface glycoprotein (G) of rhabdovirus is considered as a major antigen that could induce a primary host immune response. As the typical rhabdovirus, SVCV G protein is the most commonly protein used in SVCV vaccine constructs [13]. As a promising carrier, single-walled carbon nanotubes (SWCNTs) has been widely used for antigens and drugs delivery attributable to its excellent properties, such as biocompatibility, needle-like structure, and high carrying capacity [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Application of antigen presenting cell-targeted nanovaccine delivery system in rhabdovirus disease prophylactics using fish as a model organism

2020

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Background: Targeted delivery of virus-associated antigens to professional antigen-presenting cells (APCs) is considered as an efficient strategy to enhance the pyrophytic effect of vaccines against rhabdovirus disease. Materials and methods:In this study, we constructed a targeted carbon nanotubes-based vaccine deliver system (SWCNTs-MG) which can recognize the signature receptor (mannose) of APCs. An environmentally and economically important disease called spring viremia of carp (SVC) was studied as a model to evaluate the feasibility of singlewalled carbon nanotubes (SWCNTs) conjugated with mannosylated antigen for rhabdovirus prevention.Results: Results showed that SWCNTs-MG could cross into fish body and present to internal immune-related tissues through gill, muscle and intestine within 6 h immersed vaccination. With further modification of mannose moiety, the obtained nanovaccine showed enhanced uptake by carp macrophages and immune-related tissues, which would then trigger strong immune responses against spring viremia of carp virus (SVCV) infection. Moreover, the survival rate of fish vaccinated with SWCNTs-MG (30 mg/L) was 63.5% after SVCV infection, whereas it was 0% for the control group. Conclusion:This study not only provide a theoretical basis and research template for the application of targeted nanovaccine system in aquatic animals, but also play an important role in supporting development of healthy aquaculture and ensuring the safety of aquatic products and ecology.

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“…In fish, the most common administration routes for DNA vaccines are intraperitoneal, intramuscular, and oral 3,6,7 , while in mammals, subcutaneous and intradermal routes are additional options 2 . After an intramuscular injection, the administrated DNA plasmids enter the cells at the administration site (e.g., mostly myocytes, but also resident antigen-presenting cells [APCs]).…”

Section: Introductionmentioning

confidence: 99%

“…. Furthermore, DNA vaccination has been shown to elicit similar immune responses in fish and in mammals, including T and B cell responses 6,13,14,15,16 .…”

mentioning

confidence: 99%

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines

Myllymäki¹,

Niskanen²,

Oksanen³

et al. 2018

JoVE

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The interest in DNA-based vaccination has increased during the past two decades. DNA vaccination is based on the cloning of a sequence of a selected antigen or a combination of antigens into a plasmid, which enables a tailor-made and safe design. The administration of DNA vaccines into host cells leads to the expression of antigens that stimulate both humoral and cell-mediated immune responses. This report describes a protocol for the cloning of antigen sequences into the pCMV-EGFP plasmid, the immunization of adult zebrafish with the vaccine candidates by intramuscular microinjection, and the subsequent electroporation to improve intake. The vaccine antigens are expressed as green fluorescent protein (GFP)-fusion proteins, which allows the confirmation of the antigen expression under UV light from live fish and the quantification of expression levels of the fusion protein with ELISA, as well as their detection with a western blot analysis. The protective effect of the vaccine candidates is tested by infecting the fish with Mycobacterium marinum five weeks postvaccination, followed by the quantification of the bacteria with qPCR four weeks later. Compared to mammalian preclinical screening models, this method provides a cost-effective method for the preliminary screening of novel DNA-based vaccine candidates against a mycobacterial infection. The method can be further applied to screening DNA-based vaccines against various bacterial and viral diseases.

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Intramuscular DNA Vaccination of Juvenile Carp against Spring Viremia of Carp Virus Induces Full Protection and Establishes a Virus-Specific B and T Cell Response

Cited by 39 publications

References 72 publications

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Application of antigen presenting cell-targeted nanovaccine delivery system in rhabdovirus disease prophylactics using fish as a model organism

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines

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