Intramuscular delivery of a naked DNA plasmid encoding proinsulin and pancreatic regenerating III protein ameliorates type 1 diabetes mellitus

Reg3g overexpression promotes β cell regeneration and induces immune tolerance in nonobese-diabetic mouse model

“…Previously, we reported [15] that i.m. injections of BALB/c mice with naked plasmid encoding Reg3 significantly decreased the onset of T1D and relieved the severity of streptozotocininduced T1D.…”

“…Previously, our study [15] found that the onset of T1D could be delayed by intramuscular delivery of naked plasmids encoding proinsulin and Reg3 in streptozotocin-diabetic BALB/c mice as a gene therapy. Interestingly, this ameliorated T1D was also accompanied by decreased Th1-related cytokines, such as IFN-g and IL-2.…”

DNA Immunotherapies for Type 1 Diabetes

“…In another study, a DNA vaccine encoding proinsulin and pancreatic regenerating (Reg) III protein resulted in a significant reduction of hyperglycemia and diabetes incidence with increased serum insulin in a streptozotocin-induced mice model [64]. The treatment also restored the balance of T helper-1/T helper-2 cytokines, expanded CD4 + CD25 + Foxp3 + T regulatory cells, and attenuated insulitis by inhibiting activation of nuclear factor-kappa B (NF-κB) in the pancreas, which is thought to promote the regeneration of islet beta cells.…”

Preparation of Preproinsulin Gene Construct Containing the Metallothionein2A (pBINDMTChIns) and Its Expression in NIH3T3 Cell Line and Muscle Tissue of Alloxan Diabetic Rabbits

“…Type 1 diabetes (T1DM), is a polygenic disorder and autoimmune disease in which insulin-producing cells are destroyed by autoimmune response via T cells (1,2), leading to many biochemical and clinical problems, including hyperglycemia, kidney failure, retinopathy, and neuropathy (3). The classic treatment is using exogenous insulin, which is not sufficient to stable the blood glucose at normal level and could cause clinical complications (4,5).…”