Intramuscular administration of l-arginine boosts testicular hemodynamics, plasma concentrations of testosterone and nitric oxide in heat-stressed rams

El-Shalofy, Amr S.; Samir, Haney; El-Sherbiny, Hossam R.

doi:10.1016/j.theriogenology.2022.11.030

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…It also upregulated the expression of steroidogenesis-related genes such as steroidogenic acute regulatory protein (Star), steroidogenic factor-1 (Sf1), 17β-hydroxysteroid dehydrogenase 3 (Hsd17b3), and 17α-hydroxylase/17,20-lyase (Cyp17a1) in the testes, thereby enhancing the antioxidant system and testosterone-synthesis-related genes [ 205 ]. Similarly, intramuscular administration of 5 mg/kg L-arginine dissolved in 2 mL of normal saline significantly improved antioxidant responses and increased plasma testosterone concentrations in heat-stressed rams [ 207 ].…”

Section: Role Of Exogenous Antioxidant Supplementation In Relieving H...mentioning

confidence: 99%

Advancements in Genetic Biomarkers and Exogenous Antioxidant Supplementation for Safeguarding Mammalian Cells against Heat-Induced Oxidative Stress and Apoptosis

Khan,

Chen

et al. 2024

Antioxidants

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Heat stress represents a pervasive global concern with far-reaching implications for the reproductive efficiency of both animal and human populations. An extensive body of published research on heat stress effects utilizes controlled experimental environments to expose cells and tissues to heat stress and its disruptive influence on the physiological aspects of reproductive phenotypic traits, encompassing parameters such as sperm quality, sperm motility, viability, and overall competence. Beyond these immediate effects, heat stress has been linked to embryo losses, compromised oocyte development, and even infertility across diverse species. One of the primary mechanisms underlying these adverse reproductive outcomes is the elevation of reactive oxygen species (ROS) levels precipitating oxidative stress and apoptosis within mammalian reproductive cells. Oxidative stress and apoptosis are recognized as pivotal biological factors through which heat stress exerts its disruptive impact on both male and female reproductive cells. In a concerted effort to mitigate the detrimental consequences of heat stress, supplementation with antioxidants, both in natural and synthetic forms, has been explored as a potential intervention strategy. Furthermore, reproductive cells possess inherent self-protective mechanisms that come into play during episodes of heat stress, aiding in their survival. This comprehensive review delves into the multifaceted effects of heat stress on reproductive phenotypic traits and elucidates the intricate molecular mechanisms underpinning oxidative stress and apoptosis in reproductive cells, which compromise their normal function. Additionally, we provide a succinct overview of potential antioxidant interventions and highlight the genetic biomarkers within reproductive cells that possess self-protective capabilities, collectively offering promising avenues for ameliorating the negative impact of heat stress by restraining apoptosis and oxidative stress.

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Section: Role Of Exogenous Antioxidant Supplementation In Relieving H...mentioning

confidence: 99%

Advancements in Genetic Biomarkers and Exogenous Antioxidant Supplementation for Safeguarding Mammalian Cells against Heat-Induced Oxidative Stress and Apoptosis

Khan,

Chen

et al. 2024

Antioxidants

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“…The iNOS protein also increased testicular ischemia/reperfusion (I/R) in injured rats and induced spermatogenetic activity, seminiferous tubular diameter, and Leydig cell mass (Sangodele et al, 2021). Therefore, iNOS is involved in testicular damage via decreased tissue antioxidative defense capacity and the regulation of testicular hemodynamics (El-Shalofy et al, 2023).…”

Section: The Role Of Nos In Male Animal Reproductionmentioning

confidence: 99%

“…Similar in follicular atresia, NO produced by iNOS aslo can play the negatively affected cell death via the p38 pathway during follicle development. Therefore, iNOS is involved in testicular damage and cell death via decreased tissue antioxidative defense capacity and the regulation of testicular hemodynamics (El-Shalofy et al, 2023). In male animals, NO produced by NOS can activate cGMP-dependent PRKG1 and phosphorylate astrocytes to activate soluble cGMP, thus promoting steroid production in Leydig cells.…”

Section: Nos Pathway Involved In Animal Reproductionmentioning

confidence: 99%

Nitric oxide synthase and its function in animal reproduction: an update

Zhang,

Chen,

Guo

et al. 2023

Front. Physiol.

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Nitric oxide (NO), a free radical labile gas, is involved in the regulation of various biological functions and physiological processes during animal reproduction. Recently, increasing evidence suggests that the biological role and chemical fate of NO is dependent on dynamic regulation of its biosynthetic enzyme, three distinct nitric oxide synthase (NOS) according to their structure, location and function. The impact of NOS isoforms on reproductive functions need to be timely elucidated. Here, we focus on and the basic background and latest studies on the development, structure, importance inhibitor, location pattern, complex functions. Moreover, we summarize the exactly mechanisms which involved some cell signal pathways in the regulation of NOS with cellular and molecular level in the animal reproduction. Therefore, this growing research area provides the new insight into the important role of NOS male and female reproduction system. It also provides the treatment evidence on targeting NOS of reproductive regulation and diseases.

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“…An E2 (E2-17β) assay was performed using a competitive type of ELISA (DRG kits, Marburg, Germany), with a sensitivity of 9.8 pg/mL [31]. Nitric oxide (NO) was determined colorimetrically using a nitric oxide kit (Bio-diagnostics, Giza, Egypt) with a sensitivity of 0.24 µmol/L [32,33].…”

Section: Blood Sampling and Assessment Of E2 And No Concentrationsmentioning

confidence: 99%

Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes

Samir

El‐Sherbiny

Ahmed

et al. 2023

Animals

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The postpartum (PP) period is a crucial stage for the resumption of reproductive performance and ovarian cyclicity in dairy buffaloes. The present study aimed, for the first time, to assess the effect of the administration of estradiol benzoate (EB) on ovarian and uterine hemodynamics in PP dairy buffaloes. Eight pluriparous acyclic domestic buffaloes were enrolled in the present experiment and received a dose of 10 mg of estradiol benzoate (EB) intramuscularly 4 weeks after parturition. All animals were examined two times before EB administration (days −3, and −1) and on the day of EB administration (day 0), followed by examinations on days 1, 3, 5, 7, and 9 post-EB administration. The middle uterine artery (MUA) and ovarian artery (OA) blood flow patterns were assessed using a color Doppler ultrasound device. The reproductive parameters were (1) the cross-sectional diameters (cm) of the OA and MUA, (2) cranial uterine horn thickness (UHT; cm), and (3) hemodynamic changes within the MUA on both the ipsi- and contra-lateral sides of the previous pregnant horn and within the OA corresponding to the ovarian tissues. The examined blood flow parameters were the pulsatility index (PI), resistance index (RI), peak systolic/end-diastolic ratio (S/D), time-averaged maximum velocity (TAV; cm/s), uterine blood flow rate (BFR; bpm), and uterine blood flow volume (BFV; mL/min). Concomitantly, blood samples were collected from the coccygeal vein, and the sera were stored at −18 °C for use in estradiol (E2-17β) and nitric oxide (NO) assays. The results revealed increases in both OA and MUA cross-sectional diameter (cm) on the ipsi-lateral and contra-lateral (p < 0.05) sides within 24 h until day 9 post-treatment. The values of the RI and PI of blood flow within the OA and MUA on the ipsi-lateral and contra-lateral sides of the previous pregnancy were obviously lower (p < 0.05) at 24 h after the administration of EB, and then, started to gradually elevate, reaching the pre-treatment values on day 9 after EB administration. Both the BFR and BFV in the OA and MUA significantly increased from 24 h to 72 h after EB administration on both the ipsi-lateral and contra-lateral sides (p < 0.05); then, their values started to decrease to reach the pretreatment value on day 9 after EB administration. Both E2 and NO concentrations significantly increased (p < 0.05) from 24 h until day 3 after EB injection and then started to decline after that, reaching the pre-treatment value on day 9. In conclusion, the administration of EB enhances the ovarian and uterine blood flow concomitantly with increased levels of NO in PP dairy buffaloes.

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Intramuscular administration of l-arginine boosts testicular hemodynamics, plasma concentrations of testosterone and nitric oxide in heat-stressed rams

Cited by 8 publications

References 37 publications

Advancements in Genetic Biomarkers and Exogenous Antioxidant Supplementation for Safeguarding Mammalian Cells against Heat-Induced Oxidative Stress and Apoptosis

Advancements in Genetic Biomarkers and Exogenous Antioxidant Supplementation for Safeguarding Mammalian Cells against Heat-Induced Oxidative Stress and Apoptosis

Nitric oxide synthase and its function in animal reproduction: an update

Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes

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