Intramembranous bone tissue response to biodegradable octacalcium phosphate implant

Kikawa, T.; Kashimoto, Osamu; Imaizumi, Hideki; Kokubun, Shoichi; Сузукі, Осаму

doi:10.1016/j.actbio.2008.12.008

Cited by 74 publications

(56 citation statements)

References 58 publications

(109 reference statements)

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“…The present study did not provide complete explanation for the adverse effects of the calcium concentration on ST-2 cells. However, the tendency to reduce calcium concentration by OCP surely reflects the in vivo observation that host osteoblastic cells are stimulated by OCP if implanted in bone defects as reported previously 13,48,49) . Pi ions have been found to play multiple roles in maintaining osteoblast-like cells 18) .…”

Section: Discussionmentioning

confidence: 94%

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

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The present study investigated the role of mitogen-activated protein kinase (MAPK) signaling in osteoblastic differentiation of stromal ST-2 cells induced by synthetic octacalcium phosphate (OCP) incubation. Since our previous studies revealed that OCP consumes calcium ions in media during conversion to hydroxyapatite, the effect of the ions on ST-2 cell differentiation with or without OCP crystals was analyzed. The effect of presence or absence of MAPK inhibitors was also analyzed. OCP increased alkaline phosphatase (ALP) activity and the mRNA expression of differentiation markers via the p38 signaling pathway. The PD98059 MAPK inhibitor increased ALP activity and differentiation marker genes in cells cultured in OCP-coated wells. Reduction of calcium ions in the medium by EGTA increased the ALP activity without OCP in the presence of phosphate ion concentrations up to 7.5 mM. OCP may enhance osteoblastic differentiation through the p38 signaling pathway via the reduction of calcium ions induced by its physicochemical property.

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Section: Discussionmentioning

confidence: 94%

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

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“…Although OCP has not been observed in vascular calcifications, it has been strongly suggested as a precursor phase to biological apatite found in natural and prosthetic heart valves (Tomazic et al 1994;Nancollas and Wu 2000). In surgery, OCP is used for implantation into bone defects (Suzuki et al 2006;Kikawa et al 2009;Murakami et al 2010;Suzuki 2013). For the comprehensive information on OCP, the readers are referred to other reviews (Suzuki 2010a;Chow and Eanes 2001;Suzuki 2013).…”

Section: Dcpa (Or Dcp)mentioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

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“…OCP supports osteoblastic cell differentiation (Suzuki et al, 2006b) and has been shown to increase the in vitro expression of mRNA of associated genes, such as alkaline phosphatase (ALP) and osterix (Osx), in a dose-dependent manner (Anada et al, 2008). OCP also increases the ALP activity of host osteoblastic cells in vivo (Kikawa et al, 2009;Miyatake et al, 2009). Based on the experimental evidence to date, we hypothesised that OCP may promote MSCs to initiate bone regeneration around the implanted OCP, which has been observed with host osteoblastic cells.…”

Section: Osteogenecity Of Ocp/collagen With Mscsmentioning

confidence: 99%

The effect of synthetic octacalcium phosphate in a collagen scaffold on the osteogenicity of mesenchymal stem cells

Kawai¹,

Anada²,

Masuda³

et al. 2011

eCM

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Although the effi cacy of the in vivo osteogenic capabilities of synthetic octacalcium phosphate (OCP) crystal implantation can be explained through its stimulatory capacity for the differentiation of the host osteoblastic cell lineage, direct evidence that OCP supports bone regeneration by osteogenic cells in vivo has not been shown. Mesenchymal stem cells (MSCs) isolated from 4-week-old male Wistar rat long bones were pre-incubated in osteogenic or maintenance medium in the presence or absence of basic fi broblast growth factor (bFGF). OCP/Collagen (OCP/Col) or collagen disks were seeded with MSCs that had been pre-incubated in osteogenic medium containing bFGF, which exhibited the highest differentiation induction, and then incubated for an additional day. The disks were implanted in critical-sized calvaria defects of 12-weekold male Wistar rats and the specimens were analysed radiographically, histologically, histomorphometrically, and by micro-computed tomography (CT) imaging at 4 and 8 weeks after the implantation. The OCP/Col·MSCs group rapidly induced more bone regeneration, even within 4 weeks, compared to the OCP/Col group without MSCs. The bone mineral density of the OCP/Col·MSCs group was also greater than the OCP/Col group. The Col·MSCs group did not exhibit prominent osteogenicity. These results indicate that OCP crystals in a collagen matrix effi ciently promote exogenously introduced osteogenic cells to initiate bone regeneration if the cells are pre-treated in a suitable differentiation condition.

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Intramembranous bone tissue response to biodegradable octacalcium phosphate implant

Cited by 74 publications

References 58 publications

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

The effect of synthetic octacalcium phosphate in a collagen scaffold on the osteogenicity of mesenchymal stem cells

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