Intramammary infusion of a live culture of Lactococcus lactis in ewes to treat staphylococcal mastitis

Mignacca, S.A.; Dore, Simone; Spuria, Liliana; Zanghì, P.; Amato, Benedetta; Dupré, Ilaria; Armas, Federica; Biasibetti, Elena; Camperio, Cristina; Lollai, Stefano; Capucchio, Maria Teresa; Cannas, Eugenia Agnese; Presti, Vincenzo Di Marco Lo; Marianelli, Cinzia

doi:10.1099/jmm.0.000641

Cited by 16 publications

(14 citation statements)

References 23 publications

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“…In bovines, intramammary infusion with lactococci has been proposed to be as effective at eliminating chronic subclinical infections as an antibiotic treatment (Klostermann et al, 2008). However, rather than reconstituting the microbiota equilibrium, some authors argue that this might be due to an immune stimulation enabling a response of the mammary gland and clearance of subclinical intramammary infection agents (Crispie et al, 2008;Mignacca et al, 2017). In line with this concept, Pellegrino et al (2017) described that inoculation of lactic acid bacteria in cows at dry-off period increased the amount of IgG isotypes in blood and milk and found that these antibodies were able to recognize S. aureus epitopes.…”

Section: Can We Manipulate the Milk Microbiota In Order To Improve Mamentioning

confidence: 99%

Milk Microbiota: What Are We Exactly Talking About?

et al. 2020

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The development of powerful sequencing techniques has allowed, albeit with some biases, the identification and inventory of complex microbial communities that inhabit different body sites or body fluids, some of which were previously considered sterile. Notably, milk is now considered to host a complex microbial community with great diversity. Milk microbiota is now well documented in various hosts. Based on the growing literature on this microbial community, we address here the question of what milk microbiota is. We summarize and compare the microbial composition of milk in humans and in ruminants and address the existence of a putative core milk microbiota. We discuss the factors that contribute to shape the milk microbiota or affect its composition, including host and environmental factors as well as methodological factors, such as the sampling and sequencing techniques, which likely introduce distortion in milk microbiota analysis. The roles that milk microbiota are likely to play in the mother and offspring physiology and health are presented together with recent data on the hypothesis of an enteromammary pathway. At last, this fascinating field raises a series of questions, which are listed and commented here and which open new research avenues.

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Section: Can We Manipulate the Milk Microbiota In Order To Improve Mamentioning

confidence: 99%

Milk Microbiota: What Are We Exactly Talking About?

et al. 2020

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“…The cure rates of the two treatments were similar. Two field studies carried out on ewes were used to assess the capacity of the L. lactis LMG7930 to cure staphylococcal (CoNS or S. aureus ) infections ( 106 ). The infused glands developed an inflammatory response, which was accompanied by a transient eclipse in CoNS milk shedding followed by relapse, but no effect on S aureus infections.…”

Section: Appraisal Of Published Probiotic Trials In Relation To Mastimentioning

confidence: 99%

A Critical Appraisal of Probiotics for Mastitis Control

Rainard

Foucras

2018

Front. Vet. Sci.

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The urge to reduce antimicrobials use in dairy farming has prompted a search for alternative solutions. As infections of the mammary gland is a major reason for antibiotic administration to dairy ruminants, mammary probiotics have recently been presented as a possible alternative for the treatment of mastitis. To assess the validity of this proposal, we performed a general appraisal of the knowledge related to probiotics for mammary health by examining their potential modes of action and assessing the compatibility of these mechanisms with the immunobiology of mammary gland infections. Then we analyzed the literature published on the subject, taking into account the preliminary in vitro experiments and the in vivo trials. Preliminary experiments aimed essentially at exploring in vitro the capacity of putative probiotics, mainly lactic acid bacteria (LABs), to interfere with mastitis-associated bacteria or to interact with mammary epithelial cells. A few studies used LABs selected on the basis of bacteriocin production or the capacity to adhere to epithelial cells to perform in vivo experiments. Intramammary infusion of LABs showed that LABs are pro-inflammatory for the mammary gland, inducing an intense influx of neutrophils into milk during lactation and at drying-off. Yet, their capacity to cure mastitis remains to be established. A few preliminary studies tackle the possibility of using probiotics to interfere with the teat apex microbiota or to prevent the colonization of the teat canal by pathogenic bacteria. From the analysis of the published literature, it appears that currently there is no sound scientific foundation for the use of probiotics to prevent or treat mastitis. We conclude that the prospects for oral probiotics are not promising for ruminants, those for intramammary probiotics should be considered with caution, but that teat apex probiotics deserve further research.

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“…In a model of mouse mastitis, the experimental infection with Staphylococcus chromogenes and treatment with live cultures of Lactococcus lactis induced an increased level of IL-1β and TNFα, in addition to tissues damages, suggesting that these GRAS strains cannot be used for mastitis treatment in rodents. Recent findings on ewes affected by subclinical mastitis confirmed that the infusion of Lactococcus lactis into the mammary gland leads to a transient clearance of the pathogens, but also increases the inflammatory status of the mammary gland [24]. Similarly, treatment with different strains of Lactobacillus failed to decrease cow mastitis and caused a local inflammatory response [25,26].…”

Section: Introductionmentioning

confidence: 99%

Impact of intramammary inoculation of inactivated Lactobacillus rhamnosus and antibiotics on the milk microbiota of water buffalo with subclinical mastitis

Catozzi

Cuscó²,

Lecchi

et al. 2019

PLoS ONE

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Water buffalo mastitis represents a major issue in terms of animal health, cost of therapy, premature culling and decreased milk yeld. The emergence of antibiotic resistance has led to investigate strategies to avoid or reduce antibiotics’ based therapies, in particular during subclinical mastitis. The use of Generally Regarded As Safe bacteria (GRAS) such as Lactobacillus rhamnosus to restore the unbalance in mammary gland microbiota could provide potential corrective measures. The aim of this study was to investigate the changes in milk microbiota after the intramammary treatment with inactivated cultures of Lactobacillus rhamnosus of mammary gland quarters naturally affected by subclinical mastitis as compared to antibiotic therapy.A number of 43 quarters affected by subclinical mastitis with no signs of clinical inflammation and aerobic culture positive for pathogens were included in the study. The experimental design was as follows: 11 quarters were treated with antibiotics, 15 with inactivated cultures of Lactobacillus rhmnosus and 17 with PBS as negative control, by means of intrammary injection. Samples were collected at eight time points, pre- (T-29, T-21, T-15, T-7, T0 days) and post- treatment (T1, T2, and T6 days). Microbiological culture and Somatic Cell Count (SCC) were perfomed on all the samples, and microbiota was determined on milk samples collected at T0 and T6 by amplifying the V4 region of 16S rRNA gene by PCR and sequencing using next generation sequencing technique. Treatment with Lactobacillus rhamnosus elicited a strong chemotactic response, as determined by a significant increase of leukocytes in milk, but did not change the microbiological culture results of the treated quarters. For what concerns the analysis of the microbiota, the treatment with Lactobacillus rhamnosus induced the modification in relative abundance of some genera such as Pseudomonas and 5-7N15. As expected, antibiotic treatment caused major changes in microbiota structure with an increase of Methylobacterium relative abundance. No changes were detected after PBS treatment. In conclusion, the present findings demonstrated that the in vivo intrammmary treatment with Lactobacillus rhamnosus has a transient pro-inflammatory activity by increasing SCC and is capable to modify the microbiota of milk after six days from inoculation, albeit slightly, even when the bacterial cultures were heat inactivated. Further studies are necessary to assess the potential use of this GRAS as supportive therapy against mastitis.

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Intramammary infusion of a live culture of Lactococcus lactis in ewes to treat staphylococcal mastitis

Cited by 16 publications

References 23 publications

Milk Microbiota: What Are We Exactly Talking About?

Milk Microbiota: What Are We Exactly Talking About?

A Critical Appraisal of Probiotics for Mastitis Control

Impact of intramammary inoculation of inactivated Lactobacillus rhamnosus and antibiotics on the milk microbiota of water buffalo with subclinical mastitis

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