Intramammary infections with the contagious Staphylococcus aureus genotype B in Swiss dairy cows are associated with low prevalence of coagulase-negative staphylococci and Streptococcus spp.

Michel, A.; Syring, Claudia; Steiner, Adrian; Graber, H.U.

doi:10.1016/j.tvjl.2010.05.030

Cited by 14 publications

(21 citation statements)

References 17 publications

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“…According to our results, the prevalence of animals with udder health problems (buffaloes affected by IMI, SM, and CM) caused by the bacterium (up to 55%; Table 1) as well as the incidence of positive samples for Staph. aureus collected during the whole sampling period (20.4%) were lower than those described for cows (Michel et al, 2011). Nevertheless, considering the prevalence of CM, SM, and IMI due to the bacterium, as well as its relationship with other udder-specific pathogens isolated in the present study (Table 1), Staph.…”

Section: Discussioncontrasting

confidence: 53%

“…Genotype B was related to contagiousness and increased pathogenicity, causing herd problems with a within-herd prevalence of up to 87%, and often 2 or more quarters were affected per cow. By contrast, other genotypes were found in samples of single cows (ranging from 4 to 33.3%) with only 1 quarter per cow infected (Michel et al, 2011).…”

Section: Clinical Outcomes and Molecular Genotyping Of Staphylococcusmentioning

confidence: 77%

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Clinical outcomes and molecular genotyping of Staphylococcus aureus isolated from milk samples of dairy primiparous Mediterranean buffaloes (Bubalus bubalis)

Guccione

Cosandey

Pesce

et al. 2014

Journal of Dairy Science

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Staphylococcus aureus is one of the most important pathogens causing mastitis in dairy cows and in Mediterranean buffaloes. Genotype B (GTB) is contagious in dairy cows and may occur in up to 87% of cows of a dairy herd. It was the aim of this study to evaluate genotypes present, clinical outcomes, and prevalence of Staph. aureus in milk samples of primiparous Mediterranean dairy buffaloes. Two hundred composite milk samples originating from 40 primiparous buffaloes were collected from May to June 2012, at d 10, 30, 60, 90, and 150 d in milk (DIM) to perform somatic cell counts and bacteriological cultures. Daily milk yields were recorded. Before parturition until 40 to 50 DIM, all primiparous animals were housed separated from the pluriparous animals. Milking was performed in the same milking parlor, but the primiparous animals were milked first. After 50 DIM, the primiparous were mixed with the pluriparous animals, including the milking procedure. Individual quarter samples were collected from each animal, and aliquots of 1 mL were mixed and used for molecular identification and genotyping of Staph. aureus. The identification of Staph. aureus was performed verifying the presence of nuc gene by nuc gene PCR. All the nuc-positive isolates were subjected to genotype analysis by means of PCR amplification of the 16S-23S rRNA intergenic spacer region and analyzed by a miniaturized electrophoresis system. Of all 200 composite samples, 41 (20.5%) were positive for Staph. aureus, and no genotype other than GTB was identified. The prevalence of samples positive for Staph. aureus was 0% at 10 DIM and increased to a maximum of 22/40 (55%) at 90 DIM. During the period of interest, 14 buffaloes tested positive for Staph. aureus once, 6 were positive twice, and 5 were positive 3 times, whereas 15 animals were negative at every sampling. At 90 and 150 DIM, 7 (17.5%) and 3 buffaloes (7.5%), respectively, showed clinical mastitis (CM), and only 1 (2.5%) showed CM at both samplings. At 60, 90, and 150 DIM, 1 buffalo was found with subclinical mastitis at each sampling. At 30, 60, 90, and 150 DIM, 2.5 (1/40), 22.5 (9/40), 35 (14/40), and 10% (4/40) were considered affected by intramammary infection, respectively. Buffaloes with CM caused by Staph. aureus had statistically significantly higher mean somatic cell count values (6.06 ± 0.29, Log 10 cells/mL ± standard deviation) and statistically significantly lower mean daily milk yields (7.15 ± 1.49, liters/animal per day) than healthy animals (4.69 ± 0.23 and 13.87 ± 2.64, respectively), buffaloes with IMI (4.82 ± 0.23 and 11.16 ± 1.80, respectively), or with subclinical mastitis (5.47 ± 0.10 and 10.33 ± 0.68, respectively). Based on our knowledge, this is the first time that Staph. aureus GTB has been identified in milk samples of dairy Mediterranean buffaloes.

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Section: Discussioncontrasting

confidence: 53%

Section: Clinical Outcomes and Molecular Genotyping Of Staphylococcusmentioning

confidence: 77%

Clinical outcomes and molecular genotyping of Staphylococcus aureus isolated from milk samples of dairy primiparous Mediterranean buffaloes (Bubalus bubalis)

Guccione

Cosandey

Pesce

et al. 2014

Journal of Dairy Science

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“…Four manuscripts presented odds ratios (OR), 1 unadjusted from a case-control analysis (Lam et al, 1997) and 3 adjusted from multilevel models (Schukken et al, 1999;Green et al, 2002Green et al, , 2005, and either standard errors (SE) or 95% confidence intervals, from which SE could be calculated. Three articles (Hogan et al, 1988;Woolford et al, 2001;Zadoks et al, 2001a) reported rates of new infections, and 3 others reported differing measures such as median difference of infection at the herd level (Michel et al, 2011), clinical differences (Spencer et al, 1968), and coefficients from Poisson models (Zadoks et al, 2001b). Data from these 6 papers could not be included in the meta-analysis, although, as selections for the systematic review, their reported outcomes are discussed later in this manuscript.…”

Section: Data Extractionmentioning

confidence: 99%

“…Hogan et al (1988) reported rates of infection with minor and major pathogens, but extracting raw data from the information given was not possible. Michel et al (2011) included only median differences of minor and major pathogen infections between herds and estimated a protective effect based on herd profile. Woolford et al (2001) summarized a higher incidence of Strep.…”

Section: Descriptive Informationmentioning

confidence: 99%

“…Studies that could not be included in the meta-analysis because they did not report OR reported differing effects, with Spencer et al (1968), Hogan et al (1988), Woolford et al (2001), andZadoks et al (2001a,b) all showing either an increase or no difference in the major pathogen infection in quarters infected with minor pathogens. The paper by Michel et al (2011) describes greater prevalence of CNS in herds with lower prevalence of Staph. aureus, which might be inferred as protection by CNS against Staph.…”

Section: Meta-analysesmentioning

confidence: 99%

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Examining the effect of intramammary infections with minor mastitis pathogens on the acquisition of new intramammary infections with major mastitis pathogens—A systematic review and meta-analysis

Reyher

Haine

Dohoo

et al. 2012

Journal of Dairy Science

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Major mastitis pathogens such as Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis, Streptococcus dysgalactiae, and the coliforms are usually considered more virulent and damaging to the udder than minor mastitis pathogens such as Corynebacterium bovis and coagulase-negative staphylococci (CNS). The current literature contains several studies detailing analyses with conflicting results as to whether intramammary infection (IMI) with the minor pathogens decreases, increases, or has no effect on the risk of a quarter acquiring a new intramammary infection (NIMI) with a major pathogen. To investigate the available scientific evidence regarding the effect of IMI with minor pathogens on the acquisition of NIMI with major pathogens, a systematic review and meta-analysis were conducted. The total extant English-and Frenchlanguage literature in electronic databases was searched and all publications cited by relevant papers were investigated. Results from 68 studies were extracted from 38 relevant papers. Random-effects models were used to investigate the effects of CNS and C. bovis on acquisition of new IMI with any of the major pathogens, as well as individually for the minor pathogens and Staph. aureus. Significant heterogeneity among studies exists, some of which could be accounted for by using meta-regression. Overall, observational studies showed no effect, whereas challenge studies showed strong and significant protective effects, specifically when major pathogens were introduced into the mammary gland via methods bypassing the teat end. Underlying risk can account for several unmeasured factors, and studies with higher underlying risk found more protective effects of minor pathogens. Larger doses of challenge organisms reduced the protective effect of minor pathogens, and studies with more stringent diagnostic criteria for pathogen IMI identified less protection. Smaller studies (those utilizing fewer than 40 cows) also showed a greater protective effect than larger studies.

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Bovine Staphylococcus aureus: Diagnostic properties of specific media

Graber

Pfister

Burgener

et al. 2013

Research in Veterinary Science

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Intramammary infections with the contagious Staphylococcus aureus genotype B in Swiss dairy cows are associated with low prevalence of coagulase-negative staphylococci and Streptococcus spp.

Cited by 14 publications

References 17 publications

Clinical outcomes and molecular genotyping of Staphylococcus aureus isolated from milk samples of dairy primiparous Mediterranean buffaloes (Bubalus bubalis)

Clinical outcomes and molecular genotyping of Staphylococcus aureus isolated from milk samples of dairy primiparous Mediterranean buffaloes (Bubalus bubalis)

Examining the effect of intramammary infections with minor mastitis pathogens on the acquisition of new intramammary infections with major mastitis pathogens—A systematic review and meta-analysis

Bovine Staphylococcus aureus: Diagnostic properties of specific media

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