Intraguild predation by <i><scp>H</scp>armonia axyridis</i> (<scp>C</scp>oleoptera: <scp>C</scp>occinellidae) on native insects in <scp>E</scp>urope: molecular detection from field samples

Brown, Peter; Ingels, Brecht; Wheatley, Angela; Rhule, Emma; Clercq, Patrick De; Leeuwen, Thomas Van; Thomas, Alison

doi:10.1111/ens.12092

Cited by 43 publications

(34 citation statements)

References 21 publications

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“…All the primers designed successfully amplified the B. oleae mitochondrial COI gene fragments, with amplicon sizes ranging from 81 bp to 302 bp. Given the DNA degradation during digestive processes, amplifying small fragments of multicopy genes improves detection rates of prey DNA in predators' gut (Brown et al, 2015;MacDonald et al, 2014). The amplicons obtained are within the size range for post-digestion amplification products reported in previous surveys with other primers and other pest species: 78-242 bp (Harper et al, 2005), 160-281 bp (Harwood et al, 2007), 101-274 bp (King et al, 2010), 130-330 bp (Monzó et al, 2010), and 150-345 bp (Moreno-Ripoll et al, 2012).…”

Section: Discussionsupporting

confidence: 53%

Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera: Tephritidae)

Lantero

Matallanas

Ochando

et al. 2017

Span. j. agric. res.

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Bactrocera oleae, the olive fruit fly, is a major pest of olive (Olea europaea L.) trees worldwide. Its presence can cause important losses, with consequences for the economies of countries that produce and export table olives and olive oil. Efforts to control olive fruit fly populations have, however, been insufficient. Now more than ever, environmentally friendly alternatives need to be considered in potential control programs. Generalist predators could provide a way of managing this pest naturally. However, the identification of candidate predator species is essential if such a management system is to be introduced. The present paper describes a set of speciesspecific primers for detecting the presence of B.oleae DNA in the gut of predatory arthropods. All primers were tested for checking cross-reactive amplification of other fruit fly DNA and evaluated in heterospecific mixes of nucleic acids. All were found to be very sensitive for B. oleae. Subsequent feeding trials were conducted using one of the most abundant species of ground dwelling carabids in olive groves in south-eastern Madrid, Spain. These trials allowed determining that 253F-334R and 334F-253R primer pairs had the highest detection efficiency with an ID50 of around 78h. These primers therefore provide a very useful tool for screening the gut contents of potential predators of B. oleae, and can thus reveal candidate species for the pest's biological control.

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Section: Discussionsupporting

confidence: 53%

Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera: Tephritidae)

Lantero

Matallanas

Ochando

et al. 2017

Span. j. agric. res.

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“…It is now widespread and abundant in many regions and many habitats (Roy et al 2016). Here, we compare the invasibility of two recipient habitats (agricultural and boreal systems in Europe; see Brown et al 2015;Roy et al 2016). Harmonia axyridis engages in intraguild interactions with many species, including other aphid predators such as other ladybirds (Ware et al 2009), lacewings (Santi and Maini 2006), and hoverflies , as well as aphid parasitoids (Chacón et al 2008) and aphid-pathogenic fungi ).…”

Section: Invasibility Assessmentmentioning

confidence: 99%

Defining invasiveness and invasibility in ecological networks

et al. 2016

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The success of a biological invasion is context dependent, and yet two key concepts-the invasiveness of species and the invasibility of recipient ecosystems-are often defined and considered separately. We propose a framework that can elucidate the complex relationship between invasibility and invasiveness. It is based on trait-mediated interactions between species and depicts the response of an ecological network to the intrusion of an alien species, drawing on the concept of community saturation. Here, invasiveness of an introduced species with a particular trait is measured by its per capita population growth rate when the initial propagule pressure of the introduced species is very low. The invasibility of the recipient habitat or ecosystem is dependent on the structure of the resident ecological network and is defined as the total width of an opportunity niche in the trait space susceptible to invasion. Invasibility is thus a measure of network instability. We also correlate invasibility with the asymptotic stability of resident ecological network, measured by the leading eigenvalue of the interaction matrix that depicts traitbased interaction intensity multiplied by encounter rate (a pairwise product of propagule pressure of all members in a community). We further examine the relationship between invasibility and network architecture, including network connectance, nestedness and modularity. We exemplify this framework with a trait-based assembly model under perturbations in ways to emulate fluctuating resources and random trait composition in ecological networks. The maximum invasiveness of a potential invader (greatest Electronic supplementary material The online version of this article

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“…Brown et al . () provide recent evidence of the predation on native species by the Asian ladybird in France, England, Slovakia and the Czech Republic; Human nuisance because the beetle aggregates in buildings when seeking overwintering sites in the autumn, thousands being reported in a single household (CABI Invasive Species Compendium). Human nuisances that have been reported include aesthetic inconvenience due to aggregates in the housing).…”

Section: Background and Insights From The Literaturementioning

confidence: 99%

Valuing the Impacts of An Invasive Biological Control Agent: A Choice Experiment on the Asian Ladybird in France

Chakir¹,

David²,

Gozlan³

et al. 2016

J Agricultural Economics

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Initially used as a biological control against aphids, the Asian ladybird has become highly invasive in many regions, including Europe. While biological control is usually considered as an environmentally‐friendly alternative to chemical pesticides in controlling pests in crops, there is growing concern that these environmental benefits could be outweighed by the negative consequences of the invasion. These include (i) biodiversity losses as populations of native ladybirds suffer from intraguild predation and competition for resources; (ii) human nuisance in houses, including risks of allergy and (iii) potential losses to wine‐growers. We provide an economic valuation of environmental and private characteristics affected by the Asian ladybird's invasion. We conduct a discrete choice experiment among a representative sample of the French population. Our results show that the consequences of the Asian ladybird's introduction do affect significantly the population's welfare. Among these, the impact on biodiversity through the threat on native species appears to be a significant concern.

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Intraguild predation by Harmonia axyridis (Coleoptera: Coccinellidae) on native insects in Europe: molecular detection from field samples

Cited by 43 publications

References 21 publications

Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera: Tephritidae)

Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera: Tephritidae)

Defining invasiveness and invasibility in ecological networks

Valuing the Impacts of An Invasive Biological Control Agent: A Choice Experiment on the Asian Ladybird in France

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