Intragonadal Somatic Cells (ISCs) in the male oyster Crassostrea gigas: Morphology and contribution in germinal epithelium structure

Franco, Alban; Kellner, K; Goux, Didier; Mathieu, Michel; Berthelin, Clothilde Heude

doi:10.1016/j.micron.2011.04.003

Cited by 10 publications

(4 citation statements)

References 36 publications

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“…In stage II females, the same apparent disagreement of expression by qPCR and ISH may be explained in the same way even if tubules are bigger and filled with bigger cells. Nevertheless, a somatic expression cannot be excluded as such cells have been shown to be closely related to germ cells in male oysters [Franco et al, 2011] and are therefore difficult to distinguish from the germ cells by ISH. Moreover, isolated vitellogenic oocytes of oysters showed a low expression of CgFoxl2 mRNA compared to gonadal areas [Lelong C., pers.…”

Section: Adult Expression Of Cg-foxl2os and Cg-foxl2mentioning

confidence: 99%

“…It may play a role in key steps of the gonadic differentiation of C. gigas , (i) at the first gonadic establishment in spats of around 1-2-months of age, when primordial germ cells may differentiate into germinal stem cells and (ii) in adults for vitellogenesis in females. The role of this factor in the oyster's gonadic differentiation is still not clear for many reasons: (i) the oyster sex determination is still unknown and no molecular female-or male-specific markers are actually available; (ii) the adult gametogenesis is poorly known as no advanced study on oogenesis and only few on spermatogenesis [Franco et al, 2008[Franco et al, , 2010[Franco et al, , 2011 are available; (iii) the first gonadic development is still unknown regarding both the time-window, the factors, the cells and the mechanisms involved.…”

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confidence: 99%

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Expression of a Natural Antisense Transcript of Cg-Foxl2 during the Gonadic Differentiation of the Oyster Crassostrea gigas: First Demonstration in the Gonads of a Lophotrochozoa Species

Santerre

Sourdaine

Martinez

2012

Sex Dev

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In the oyster Crassostrea gigas, a successive hermaphrodite, Cg-Foxl2, an ortholog of Foxl2, is suspected to be involved in vitellogenesis or female sex determination. The existence of a natural antisense transcript (NAT) of this factor has been suspected in gonads but needs to be confirmed to better understand the early events of the gonadic differentiation. The occurrence of this NAT was studied by orientation-specific RT-PCR. The NAT and its mRNA expressions were investigated during the development and in adults by real-time PCR and in situ hybridization. The presence of stable in vivo RNA-RNA duplexes was also explored by RNase protection-based approach. This work is the first evidence of characterization of a NAT in the gonads of mollusks and Lophotrochozoa. This NAT named Cg-Foxl2os is supposed to be polyadenylated and forms RNA-RNA duplexes with its mRNA. Cg-Foxl2os is significantly more expressed than Cg-Foxl2 in 2-month-old spats and in mature males. It is co-localized with the mRNA in the cytoplasm of spermatogonia and spermatocytes. The results of this study demonstrate the existence of a NAT of Cg-Foxl2 in the gonads of C. gigas. It may regulate its mRNA expression through formation of cytoplasmic RNA-RNA duplexes during the oyster gonadic differentiation.

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Section: Adult Expression Of Cg-foxl2os and Cg-foxl2mentioning

confidence: 99%

mentioning

confidence: 99%

Expression of a Natural Antisense Transcript of Cg-Foxl2 during the Gonadic Differentiation of the Oyster Crassostrea gigas: First Demonstration in the Gonads of a Lophotrochozoa Species

Santerre

Sourdaine

Martinez

2012

Sex Dev

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“…These cells have a crucial role on the structuring of germinal epithelium in gonadal tubule. In male they possess some ultrastructural characteristics founded in vertebrate Sertoli cells (Franco et al, 2011). Therefore, these properties make ISC ideal candidates as paracrine regulator of germ lineage development.…”

Section: Discussionmentioning

confidence: 99%

Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas

Barranger

Heude-Berthelin

Rouxel

et al. 2016

Comparative Biochemistry and Physiology Part C: Toxicology &

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Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography coupled with UV and electrochemical detection analysis showed an increase in 8-oxodGuo levels in both male and female gonads after exposure to diuron. Immunohistochemistry analysis showed the presence of 8-oxodGuo at all stages of male germ cells, from early to mature stages. Conversely, the oxidized base was only present in early germ cell stages in female gonads. These results indicate that male and female genitors underwent oxidative stress following exposure to diuron, resulting in DNA oxidation in both early germ cells and gametes, such as spermatozoa, which could explain the transmission of diuron-induced DNA damage to offspring. Furthermore, immunostaining of early germ cells seems indicates that damages caused by exposure to diuron on germ line not only affect the current sexual cycle but also could affect future gametogenesis.

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“…Thus, various scales (i.e. inactive, developing, maturing, mature, and spent) categorizing gonadal alterations throughout the reproductive cycle are reported in the bivalve literature (see Lubert, 1959;Shafee, 1989;Saucedo & Southgate, 2008;Franco et al, 2011;Boltovskoy et al, 2015 for review). Despite reporting the same event, reproductive cycle scales vary depending The ovarian tubules present a reduced lumen.…”

Section: Regressing (Cession Of the Release Of Sperm)mentioning

confidence: 99%

Cellular development of the germinal epithelium during the gametogenic cycle of the golden mussel Limnoperna fortunei (Bivalvia: Mytilidae)

Tós

Quagio-Grassiotto

Mazzoni

2016

RBT

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Abstract:The golden mussel Limnoperna fortunei is an invasive species that has quickly dispersed and colonized several potential different habitats distributed all over the world, causing environmental and economic impacts. Thus, in order to contribute to a better understanding of the reproductive aspects of L. fortunei, we described the cellular dynamic of the male and female germinal epithelium during the annual reproductive life history of this species, with the use of high resolution histology. An approximate of 1 200 specimens of L. fortunei were sampled periodically on the upper Paraná River floodplain (Brazil), from March 2010 to December 2012. Based on the activity of the germinal epithelium and consequent germ cell development, this study has resulted in the recognition of the following reproductive phases: Developing, Spawning Capable, Regressing and Regenerating. In the characterization of these phases, the following germ cells were described for males: spermatogonia, primary and secondary spermatocytes, spermatids and spermatozoa. Cell nests, oogonia, early prophase oocytes, previtellogenic oocytes and vitellogenic oocytes (early vitellogenic oocytes, middle vitellogenic oocytes and full-grown oocytes) were described for females. The morphological data and reproductive parameters obtained, showed the value of the cellular dynamics of the germinal epithelium, for the understanding of the cyclic gonadal events during the adult reproductive life of the mollusk in general. These results on the gametogenesis of this invasive species may be a fundamental tool for the development of control strategies and programs implementation, to reduce their proliferation and impacts in natural local environments. Rev. Biol. Trop. 64 (2): 521-536. Epub 2016 June 01.

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Intragonadal Somatic Cells (ISCs) in the male oyster Crassostrea gigas: Morphology and contribution in germinal epithelium structure

Cited by 10 publications

References 36 publications

Expression of a Natural Antisense Transcript of Cg-Foxl2 during the Gonadic Differentiation of the Oyster <b><i>Crassostrea gigas</i></b>: First Demonstration in the Gonads of a Lophotrochozoa Species

Expression of a Natural Antisense Transcript of Cg-Foxl2 during the Gonadic Differentiation of the Oyster <b><i>Crassostrea gigas</i></b>: First Demonstration in the Gonads of a Lophotrochozoa Species

Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas

Cellular development of the germinal epithelium during the gametogenic cycle of the golden mussel Limnoperna fortunei (Bivalvia: Mytilidae)

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