Intraglandular transplantation of adipose‑derived stem cells combined with platelet‑rich fibrin extract for the treatment of irradiation‑induced salivary gland damage

Abstract

Abstract. The aim of the present study was to determine the effect of adipose-derived mesenchymal stem cells (ADSCs) combined with heterologous platelet-rich fibrin extract (PRFe) on irradiation-induced salivary gland (SG) damage. ADSCs were isolated from C3H mice, whereas PRFe was obtained from New Zealand rabbits. Twelve weeks post irradiation, the ADSCs or PRFe or their combination were transplanted into the submandibular glands of C3H mice with irradiation-induced damage. The salivary flow rate (SFR) was determined and histopathological analysis was performed at 12 weeks post transplantation. Immunofluorescence, microvessel density measurements and transmission electron microscopy were performed to assess α-amylase (AMY) production, apoptosis and microstructural changes in the cells. The administration of ADSCs combined with PRFe increased the SFR at 12 weeks post transplantation, whereas ADSCs alone or PRFe alone failed to do so. The ADSCs+PRFe-treated, irradiated SGs had fewer damaged and atrophied acinar cells, higher AMY levels and an increased microvessel density compared with the untreated irradiated SGs. Moreover, SG tissue from the ADSCs+PRFe group also showed decreased apoptotic and increased proliferative activity compared to that from the irradiated group. In conclusion, ADSCs or PRFe alone did not restore permanent, irradiation-induced damage of SG tissue when used alone, but when used together, they provided effective treatment outcomes.

Regenerative Potential of Platelet-Rich Fibrin Releasate Combined with Adipose Tissue–Derived Stem Cells in a Rat Sciatic Nerve Injury Model

“…Their exogenous addition can further potentiate undifferentiated stem cells to proliferate and differentiate and have been used for tissue regeneration purposes 19 . A recent study has shown that the use of ADSCs in conjunction with PRF extract could enhance tissue regeneration 20 . Our earlier work tested the addition of PRF releasate (PRFr) to ADSCs implantation for osteochondral defect repair, which resulted in positively influencing cartilage repair in terms of the improvement of macroscopic and histological grading scores 21 .…”

Therapeutic benefits of factors derived from stem cells from human exfoliated deciduous teeth for radiation-induced mouse xerostomia

“…It has been shown that intravenously administered MSCs accumulate in the parotid and submandibular glands and differentiate into acinar cells 18 , 20 . They also promote microvascularization and acinar cell proliferation and inhibit apoptotic cell death 28 , 29 . However, their survival rate is reportedly less than 9%, which suggests that much of the therapeutic effect of MSCs is attributable to paracrine factors from the MSCs 19 .…”

Transplantation of Bone Marrow-Derived Mesenchymal Stem Cells Preserve the Salivary Glands Structure after Head and Neck Radiation in Rats

“…The same dose was used in the model created by Fang et al, [14]. We choose to use intraglandular route this was by [10], [15] to ensure homing and detect the early changes and the ability of the treatment to preserve the structure. We found that radiation caused a remarkable decrease in the acinar field area as well as acinar diameter, a remarkable increase in fibrosis and a significant decrease in proliferation activity.…”