Intragastric pressure during food intake: a physiological and minimally invasive method to assess gastric accommodation

Janssen, Pieter; Verschueren, Sofie; Ly, Huynh Giao; Vos, Rita De; Oudenhove, Lukas Van; Tack, Jan

doi:10.1111/j.1365-2982.2011.01676.x

Cited by 80 publications

(150 citation statements)

References 34 publications

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“…Also, humans reduce food intake upon PP, accompanied by lower hunger scores (5). In healthy volunteers, pharmacologically induced impaired GA was associated with early satiation and reduced food intake (16). Impaired accommodation and early satiety were also correlated in functional dyspepsia patients (36).…”

mentioning

confidence: 97%

Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats

Verschueren

Janssen

Oudenhove

et al. 2014

American Journal of Physiology-Gastrointestinal and Liver Physi

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Verschueren S, Janssen P, Van Oudenhove L, Hultin L, Tack J. Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats. Am J Physiol Gastrointest Liver Physiol 307: G122-G128, 2014. First published April 17, 2014 doi:10.1152/ajpgi.00043.2014 is an anorexigenic hormone released from pancreatic F cells upon food intake. We aimed to determine the effect of PP on gastric accommodation and gastric emptying in conscious Wistar HAN rats to investigate whether effects on motor function could contribute to its anorexigenic effects. Intragastric pressure (IGP) was measured through a chronically implanted gastric fistula during the infusion of a nutrient meal (Nutridrink; 0.5 ml/min). Rats were treated with PP (0, 33 and 100 pmol·kg Ϫ1 ·min Ϫ1 ) in combination with N G -nitro-L-arginine methyl ester (L-NAME; 180 mg·kg Ϫ1 ·h Ϫ1 ), atropine (3 mg·kg Ϫ1 ·h Ϫ1 ), or vehicle. Furthermore, the effect of PP was tested after subdiaphragmal vagotomy of the stomach. Gastric emptying of a noncaloric and a caloric meal after treatment with 100 pmol·kg Ϫ1 ·min Ϫ1 PP or vehicle was compared using X-rays. PP significantly increased IGP during nutrient infusion compared with vehicle (P Ͻ 0.01). L-NAME and atropine significantly increased IGP during nutrient infusion compared with vehicle treatment (P Ͻ 0.005 and 0.01, respectively). The effect of PP on IGP during nutrient infusion was abolished in the presence of L-NAME and in the presence of atropine. In vagotomized rats, PP increased IGP compared with intact controls (P Ͻ 0.05). PP significantly delayed gastric emptying of both a noncaloric (P Ͻ 0.05) and a caloric (P Ͻ 0.005) meal. PP inhibits gastric accommodation and delays gastric emptying, probably through inhibition of nitric oxide release. These results indicate that, besides the well-known centrally mediated effects, PP might decrease food intake through peripheral mechanisms. gastric pressure; nutrient tolerance; nitric oxide; vagus nerve BETWEEN MEALS, GASTRIC SMOOTH muscle maintains a high resting tone because of the myoelectrical properties of the smooth muscle but also because of a constant cholinergic input from the vagal nerves. The stomach is known to relax upon food intake. This reflex relaxation, also referred to as gastric accommodation (GA), enables the stomach to receive large quantities of food without an increase in intragastric pressure (IGP) (35). Relaxation of the stomach upon food intake is mediated via vago-vagal reflex pathways, eventually leading to activation of mainly nitrergic nerves in the enteric nervous system (38). Nitric oxide (NO) finally relaxes the smooth muscle cells, and the gastric tone decreases (19).We recently described a method to assess GA in rats by measuring the IGP during intragastric infusion of a liquid test meal (15). During infusion of a test meal, IGP increased initially until an inflection point was reached, after which the IGP stabilized despite further gastric distension. It was suggested that this inflection point represents the onset of...

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mentioning

confidence: 97%

Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats

Verschueren

Janssen

Oudenhove

et al. 2014

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…To study the involvement of Y 2 receptors when they could be activated by endogenous PYY 3-36 , we performed experiments in rats that were pretreated with nutrient drink 60 min before the intragastric nutrient infusion started to mimic fed conditions. After this pretreatment, the IGP was significantly lower during nutrient infusion compared with the fasted state, indicating that the stomach is more relaxed, as could be expected when the gastric accommodation reflex is maximally activated (32). Also in the fed state, exogenous PYY 3-36 could increase IGP, and this effect could be blocked by JNJ31020028 and BIIE0246, indicating that Y 2 receptor activation could increase gastric tone both in the fed and fasted state.…”

Section: Discussionmentioning

confidence: 85%

“…We (28,32) have previously seen a close correlation between IGP during food intake and the nutrient volume required to induce maximal satiation in healthy volunteers. This finding might provide an alternative explanation for the anorectic effects of PYY in obese subjects (7).…”

Section: Discussionmentioning

confidence: 87%

Role of Y₂ receptors in the regulation of gastric tone in rats

Janssen

Verschueren

Rotondo

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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We set out to determine the effect of peptide YY(3-36) (PYY(3-36)) on the gastric muscle tone in conscious rats by measuring intragastric pressure (IGP) during intragastric nutrient drink infusion. After an overnight fast, a chronically implanted gastric fistula was connected to a custom-made nutrient drink infusion system and a catheter to measure IGP. IGP was measured before and during the infusion of a nutrient drink (Nutridrink; 0.5 ml/min) until 10 ml was infused. Rats were treated with PYY(3-36) (0, 33, and 100 pmol·kg(-1)·min(-1)) in combination with a subcutaneous injection of the Y(2) receptor antagonists JNJ31020028 (10 mg/kg) or BIIE0246 (2 mg/kg). Experiments were also performed after subdiaphragmatic vagotomy and after pretreatment with 3 ml of nutrient drink (to mimic a fed state). IGP was compared as the average IGP during nutrient infusion, represented as means ± SE and compared using ANOVA. PYY(3-36) dose dependently increased the IGP during nutrient infusion (4.7 ± 0.3, 5.7 ± 0.5 and 7.3 ± 0.7 mmHg; P < 0.01) while JNJ31020028 and BIIE0246 could block this increase [4.4 ± 0.5 (P < 0.001) and 4.8 ± 0.4 (P < 0.05) mmHg, respectively]. Also in vagotomized rats, PYY(3-36) was able to significantly increase the IGP during, an effect attenuated by JNJ31020028. BIIE0246 and JNJ31020028 were not able to decrease the IGP when no PYY(3-36) was administered. PYY(3-36) increased gastric tone through an Y(2) receptor-mediated mechanism that does not involve the vagus nerve. Y(2) receptor antagonists were not able to decrease gastric tone without exogenous administration of PYY(3-36), indicating that Y(2) receptors do not play a crucial role in the determination of gastric tone in physiological conditions.

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“…Although it was necessary to leave the bag in the stomach of the animals before the experiments and the isobaric method could not be performed, our method has the merit of allowing experiments on conscious animals. New methods for examining gastric accommodation in rats and humans were also established by measuring intragastric pressure with a manometer (Janssen et al, 2008(Janssen et al, , 2011.…”

Section: Discussionmentioning

confidence: 99%

Role of Transient Receptor Potential Ankyrin 1 in Gastric Accommodation in Conscious Guinea Pigs

Koseki

Oshima²,

Kondo³

et al. 2012

J Pharmacol Exp Ther

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We report the establishment of a new model for measuring gastric tone and liquid meal-induced accommodation in conscious guinea pigs and the role played by transient receptor potential ankyrin 1 (TRPA1). An indwelling polyethylene bag was placed in proximal stomachs of 5-week-old male Hartley guinea pigs. Gastric tone was measured by distending the bag and recording changes in intrabag pressure at various volumes. Gastric accommodation was measured by administering liquid meals and recording intrabag pressure over time. N -nitro-Larginine methyl ester hydrochloride (L-NAME) (a nitric-oxide synthase inhibitor), atropine sulfate (atropine) (a muscarinic receptor antagonist), allyl isothiocyanate (AITC) (a TRPA1 agonist), or theophylline-7-(N-4-isopropylphenyl) acetamide (HC-030031) (a selective TRPA1 antagonist) was administered 15 to 60 min before measurement. Gastric tone was increased by stepwise distension of the bag and was further significantly increased by L-NAME and significantly decreased by atropine. A liquid meal (15% w/v; 1.7 kcal) significantly decreased intrabag pressure 5 to 20 min after administration, indicating gastric accommodation; this was completely suppressed by L-NAME and further enhanced by atropine. AITC significantly increased gastric tone; this increase was decreased by HC-030031 and atropine. A combination of AITC and L-NAME significantly increased gastric tone compared with L-NAME alone. HC-030031 alone significantly decreased gastric tone. Liquid mealinduced gastric accommodation was significantly suppressed by pretreatment with AITC. We established a new model for measuring gastric tone and accommodation in conscious guinea pigs. TRPA1 activation suppresses gastric accommodation by increasing gastric tone through cholinergic neuronal pathways.

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Intragastric pressure during food intake: a physiological and minimally invasive method to assess gastric accommodation

Abstract: The IGP during nutrient drink ingestion provides a minimally invasive alternative to the barostat for the assessment of gastric accommodation. These findings furthermore indicate that IGP is a major determinant of satiation.

Cited by 80 publications

References 34 publications

Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats

Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats

Role of Y₂ receptors in the regulation of gastric tone in rats

Role of Transient Receptor Potential Ankyrin 1 in Gastric Accommodation in Conscious Guinea Pigs

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Intragastric pressure during food intake: a physiological and minimally invasive method to assess gastric accommodation

Abstract: The IGP during nutrient drink ingestion provides a minimally invasive alternative to the barostat for the assessment of gastric accommodation. These findings furthermore indicate that IGP is a major determinant of satiation.

Cited by 80 publications

References 34 publications

Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats

Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats

Role of Y2 receptors in the regulation of gastric tone in rats

Role of Transient Receptor Potential Ankyrin 1 in Gastric Accommodation in Conscious Guinea Pigs

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Role of Y₂ receptors in the regulation of gastric tone in rats