SUMMARY We investigated whether chronic subcutaneous infusion of neurotensin during 14 days would affect pancreatic and gastric growth of rats. In another experiment, neurotensin (836 pmol/ kg) was injected intraperitoneally three times a day for three days in 12 rats. Thereafter, pancreatic DNA and in vitro incorporation of 3H-thymidine into pancreatic DNA was determined. Long term infusion of 282 pmol/kg neurotensin induced an increase of pancreatic weight, DNA, and pancreatic polypeptide, whereas pancreatic protein, RNA, amylase and lipase contents were not increased. In relation to DNA, even these parameters were significantly depressed. Insulin remained unchanged. Neurotensin, therefore, caused hyperplasia of the pancreas. Intraperitoneal injection of neurotensin induced an increase of pancreatic DNA content and stimulated 3H-thymidine incorporation into DNA, whereas caerulein only augmented 3H-thymidine incorporation. Moreover, long term neurotensin infusion led to a rise in protein concentration and an increase in the thickness of the gastric antrum; antral DNA concentration was insignificantly stimulated. Neurotensin, therefore, can act as a trophic factor on pancreas and gastric antrum of the rat.One of the many actions of neurotensin is its stimulatory effect on exocrine pancreatic secretion.13 Because other pancreatic secretagogues, such as cholecystokinin and secretin, also stimulate pancreatic growth, we investigated the effect of chronic infusion of neurotensin on pancreatic growth. As neurotensin also affects gastric secretion and motility, we further examined the effect of long term administration of neurotensin on the stomach.
Methods
ANIMALSAlzet osmotic minipumps delivering 0 465 + 0-023 ,u/ h (M + SD) for 14 days were prepared to pump 43 or 282 pmol/kg/min neurotensin subcutaneously in 20 male Wistar rats. The control group consisted of 10 Wistar rats from the same litter in which plastic cylinders with the same size and shape as the minipumps had been implanted. Fourteen days after implantation, the rats were killed by neck dislocation, the pancreata and stomachs were removed, weighed, and frozen. Blood taken by cardiac puncture was centrifuged, the plasma was stored at -20°C until assayed. The frozen tissue was homogenized in