Intraepithelial and Invasive Squamous Cell Lesions of the Uterine Cervix

Crum, Christopher P.; Taylor, Peyton T.

doi:10.1007/978-1-4613-9086-2_5

Cited by 1 publication

(1 citation statement)

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“…We studied 142 cervical uterine biopsy specimens histopathologically consistent with HPV infection and diagnosed as low grade squamous intraepithelial lesions (n = 90), equivalent to cervical intraepithelial neoplasia grade I (CIN I), and high grade squamous intraepithelial lesions (n = 52), equivalent to CIN grades II and III, in accordance with previously described criteria. 9 An IMCO 10 image analyser (Kontron Bildanalyze) was used for image analysis, incorporating the Microm Image Processing program (MIP, Microm, Spain). Images were captured in real colour by a Hitachi KP-C503 TV camera linked to a Nikon microscope with a x 10 lens, and digitalised in a matrix measuring 5122 pixels.…”

Section: Methodsmentioning

confidence: 99%

Diagnosis of genital infection caused by human papillomavirus using in situ hybridisation: the importance of the size of the biopsy specimen.

Gómez¹,

Corcuera²,

Muñoz³

et al. 1995

Journal of Clinical Pathology

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Aim-To determine the size of a cervical biopsy specimen with human papillomavirus (HPV) infection required to enable in situ hybridisation to be carried out with a guarantee of a reliable result. Methods-In situ hybridisation was carried out in 142 cervical uterine biopsy specimens classified histologically as low grade and high grade squamous intraepithelial lesions. Epithelial length at the level of the basal membrane was measured by image analysis. The specimens were divided into 10 groups based on epithelial length. Results-Of the biopsy specimens, 61-2% were HPV positive. In specimens with an epithelial length below 5 mm 31-9% were HPV positive; in those between 5 and 9 mm in length 67*5% were HPV positive; and in those greater than 9 mm in length 81-8% were positive for HPV. For low grade squamous intraepithelial lesions (n = 90), 68*4% of specimens with an epithelial length greater than 5 mm were HPV positive. For high grade squamous intraepithelial lesions (n = 52), 86-8% of specimens with an epithelial length greater than 5 mm were HPV positive. Conclusions-For a diagnosis of HPV infection using in situ hybridisation, the minimum length of epithelium in a cervical biopsy specimen should be 5 mm. For high grade squamous intraepithelial lesions, specimens over 5 mm in length are suitable. For low grade squamous intraepithelial lesions, to minimise the number of false negative results, the ideal minimum length is 10 mm.

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Section: Methodsmentioning

confidence: 99%