Intraductal Injection of Lentivirus Vectors for Stably Introducing Genes into Rat Mammary Epithelial Cells in Vivo

Bu, Wen; Li, Yi

doi:10.1007/s10911-020-09469-w

Cited by 12 publications

(5 citation statements)

References 46 publications

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“…5 C ) . Genetic engineering has further paved the way for models recapitulating specific molecular BC subtypes and mutational signatures, and for studying the impact of specific germline or somatic mutations in tumor suppressor genes or oncogenes associated with BC [ 262 , 270 , 303 , 304 ], such as the creation of rats with germline Nf1 mutations using CRISPR/Cas9 gene editing [ 270 ] ( Fig. 5 F ) .…”

Section: Applicationsmentioning

confidence: 99%

“…Such a tool enables specific anatomical targeting with the retroviral constructs, which only incorporate into the genome of proliferating mammary ductal epithelial cells [258]. In addition to retroviruses, lentivirus and adeno-associated virus (AAV) vectors can be used to deliver genetic content and trigger in situ genome editing in rats, as done in mouse models [100,262].…”

Section: Genetically Engineered Rat Mammary Tumor Modelsmentioning

confidence: 99%

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Rat Models of Hormone Receptor-Positive Breast Cancer

Nicotra,

Lutz,

Messal

et al. 2024

J Mammary Gland Biol Neoplasia

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Hormone receptor-positive (HR+) breast cancer (BC) is the most common type of breast cancer among women worldwide, accounting for 70–80% of all invasive cases. Patients with HR+ BC are commonly treated with endocrine therapy, but intrinsic or acquired resistance is a frequent problem, making HR+ BC a focal point of intense research. Despite this, the malignancy still lacks adequate in vitro and in vivo models for the study of its initiation and progression as well as response and resistance to endocrine therapy. No mouse models that fully mimic the human disease are available, however rat mammary tumor models pose a promising alternative to overcome this limitation. Compared to mice, rats are more similar to humans in terms of mammary gland architecture, ductal origin of neoplastic lesions and hormone dependency status. Moreover, rats can develop spontaneous or induced mammary tumors that resemble human HR+ BC. To date, six different types of rat models of HR+ BC have been established. These include the spontaneous, carcinogen-induced, transplantation, hormone-induced, radiation-induced and genetically engineered rat mammary tumor models. Each model has distinct advantages, disadvantages and utility for studying HR+ BC. This review provides a comprehensive overview of all published models to date.

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Section: Applicationsmentioning

confidence: 99%

Section: Genetically Engineered Rat Mammary Tumor Modelsmentioning

confidence: 99%

Rat Models of Hormone Receptor-Positive Breast Cancer

Nicotra,

Lutz,

Messal

et al. 2024

J Mammary Gland Biol Neoplasia

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“…At the same time, one might wonder how well these models currently recapitulate human physiology. As just one example, it has been notoriously difficult to model ER + breast cancer in GEMMs, which is why some labs are now – also aided by the developments in genome editing technology – switching to rats [ 158 , 159 ].…”

Section: Five Challenges For the Next Decadementioning

confidence: 99%

Imagine beyond: recent breakthroughs and next challenges in mammary gland biology and breast cancer research

Amerongen

Bentires‐Alj

Boxtel

et al. 2023

J Mammary Gland Biol Neoplasia

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On 8 December 2022 the organizing committee of the European Network for Breast Development and Cancer labs (ENBDC) held its fifth annual Think Tank meeting in Amsterdam, the Netherlands. Here, we embraced the opportunity to look back to identify the most prominent breakthroughs of the past ten years and to reflect on the main challenges that lie ahead for our field in the years to come. The outcomes of these discussions are presented in this position paper, in the hope that it will serve as a summary of the current state of affairs in mammary gland biology and breast cancer research for early career researchers and other newcomers in the field, and as inspiration for scientists and clinicians to move the field forward.

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“…Therefore, intraductal injection of a virus carrying gRNA could be used to mutate NF1 and other genes associated with human ER+ cancer to generate somatic models of ER+ cancer in rats. Wen and Yi also described the intraductal injection of lentiviral vector FUCGW carrying the mutated oncogene HrasQ61L to Sprague/Dawley rats led to mammary tumors with high positive expression of both ER and PR ( 124 ). This technique is an efficient tool for modeling formation, prevention, and treatment of human breast cancer, especially ER+ breast cancer.…”

Section: Challenges In Modeling Er+ Breast Cancermentioning

confidence: 99%

Advances in Rodent Models for Breast Cancer Formation, Progression, and Therapeutic Testing

Zhang

et al. 2021

Front. Oncol.

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Breast cancer is a highly complicated disease. Advancement in the treatment and prevention of breast cancer lies in elucidation of the mechanism of carcinogenesis and progression. Rodent models of breast cancer have developed into premier tools for investigating the mechanisms and genetic pathways in breast cancer progression and metastasis and for developing and evaluating clinical therapeutics. Every rodent model has advantages and disadvantages, and the selection of appropriate rodent models with which to investigate breast cancer is a key decision in research. Design of a suitable rodent model for a specific research purpose is based on the integration of the advantages and disadvantages of different models. Our purpose in writing this review is to elaborate on various rodent models for breast cancer formation, progression, and therapeutic testing.

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Intraductal Injection of Lentivirus Vectors for Stably Introducing Genes into Rat Mammary Epithelial Cells in Vivo

Cited by 12 publications

References 46 publications

Rat Models of Hormone Receptor-Positive Breast Cancer

Rat Models of Hormone Receptor-Positive Breast Cancer

Imagine beyond: recent breakthroughs and next challenges in mammary gland biology and breast cancer research

Advances in Rodent Models for Breast Cancer Formation, Progression, and Therapeutic Testing

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