Intradetrusor botulinum neurotoxin A (BoNT‐A) injections decrease bladder fibrosis secondary to partial urethral obstruction in the male rat model

Abstract

Partial urethral obstruction induces increased bladder tissue contractile responses to neurogenic and pharmacological stimulation and intradetrusor BoNT-A injections decrease these responses at 5th days after injection. As the unique finding of this study, intradetrusor BoNT-A injections appear to decrease bladder fibrosis secondary to PUO in the male rat model.

The effect of intradetrusor botulinum neurotoxin type A on urinary NGF, TGF BETA-1, TIMP-2 levels in children with neurogenic detrusor overactivity due to myelodysplasia

“…Present study has shown a reduction in urinary TGF Beta‐1 levels following BoNT‐A treatment. One explanation for this condition could be a reduction in bladder fibrosis as it is expected after BoNT‐A treatment …”

“…Detrusor muscle hypertrophy and fibrosis develops in NDOA. Reports from numerous experimental and clinical studies have indicated that intradetrusor BoNT‐A injection reduces the development of detrusor hypertrophy and fibrosis . It is likely that the fall in urine NGF levels following intradetrusor injection of BoNT‐A may be related to its last mentioned effect.…”

The effect of doxazosin and sildenafil citrate combination on bladder tissue contractility, alpha adrenergic receptor, and iNOS subtype expression in a male rat model of partially bladder outlet obstruction

“…A sterile metal bar with a 0.91 mm diameter was placed on the prostatic urethral surface, and a 3‐0 polypropylene suture was used to place a tie around both the prostatic urethra and the bar. As soon as the suture was secured, the bar was removed, leaving the prostatic urethra partially obstructed . Sham operations were also done in the same manner and after the dissection and isolation of the urethra from the cavernous bodies incision was closed without creating any obstruction on the urethra.…”

“…Tissue strips were progressively stretched and periodically contracted with electrical field stimulation (EFS) until optimal isometric tension was achieved. Bladder tissue strips were then subjected to different protocols, as contraction and relaxation studies as described in below …”

“…There is no perfect animal model mimicking bladder outlet obstruction due to prostatic enlargement, especially with rats. The animal model that we used is a previously studied model but the biomechanical response to distal urethral obstruction is markedly different from a proximal prostatic obstruction . This type of obstruction is not particularly relevant to clinical prostatic obstruction and might result in a bias.…”