Intradermal immunization with inactivated swine influenza virus and adjuvant polydi(sodium carboxylatoethylphenoxy)phosphazene (PCEP) induced humoral and cell-mediated immunity and reduced lung viral titres in pigs

Magiri, Royford; Lai, Ken; Chaffey, Alyssa; Zhou, Yan; Pyo, Hyun-Mi; Gerdts, Volker; Wilson, Heather L.; Mutwiri, George

doi:10.1016/j.vaccine.2018.02.026

Cited by 16 publications

(26 citation statements)

References 29 publications

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“…Previously, we showed that pigs immunized intradermally, with inactivated H1N1 swine influenza virus were protected against homologous challenge [ 13 ]. Here, animals were challenged with heterologous SIV H3N2 virus to determine whether the PCEP-adjuvanted H1N1 vaccine was cross-protective.…”

Section: Resultsmentioning

confidence: 99%

“…We have reported that the experimental adjuvant PCEP promotes strong antigen-specific Th1- and Th2-type immune responses to influenza antigens in mice and pigs [ 10 , 13 , 14 ]. Further, intradermal administration of an inactivated H1N1 SIV vaccine formulated with PCEP in pigs induced both systemic (both humoral and cell-mediated) and mucosal immune responses as indicated by reduced lung viral titres in pigs challenged with homologous H1N1 virus [ 13 ]. The broad-spectrum immune responses induced by PCEP-adjuvanted influenza vaccine suggested to us that this adjuvant can confer cross-protection.…”

Section: Discussionmentioning

confidence: 99%

“…Inactivated SIV vaccines were diluted with PBS (pH of 7.4) into 250 μL per injection site. The highest dose of PCEP was chosen based on the previous experiments using PCEP in pigs [ 13 , 17 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…This cross-protection was apparently mediated via induction of Th1-mediated antibody responses [ 7 ]. Polyphosphazenes are high-molecular weight, water-soluble polymers that have been shown to promote and enhance long lasting immune responses with a variety of viral and bacterial antigens [ 8 , 9 , 10 , 11 , 12 , 13 ]. Poly (di (sodium carboxylatoethylphenoxy))-phosphazene (PCEP) promotes strong antigen-specific Th1- and Th2-type immune responses to influenza antigens in mice and pigs [ 10 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…PCEP induced significant production of interleukin IL-1β, and IL-13 at the site of injection and IL-1β, and IL-6 at the draining lymph nodes. and we previously reported that pigs vaccinated via the intradermal route with PCEP-adjuvanted inactivated SIV H1N1 vaccine were protected against virulent challenge with homologous H1N1 [ 13 ]. PCEP injected intradermally into pigs in the absence of antigen induced significant production of interleukin IL-1β, and IL-13 at the site of injection and IL-1β, and IL-6 at the draining lymph nodes, possibly contributing to an immunocompetent environment [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Experimental PCEP-Adjuvanted Swine Influenza H1N1 Vaccine Induced Strong Immune Responses but Did Not Protect Piglets against Heterologous H3N2 Virus Challenge

et al. 2020

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Vaccination is the most efficient method of protection against influenza infections. However, the rapidly mutating viruses and development of new strains make it necessary to develop new influenza vaccines annually. Hence, vaccines that stimulate cross-protection against multiple influenza subtypes are highly sought. Recent evidence suggests that adjuvants such as PCEP that promote Th1-type T cell and Th2-type T cell immune responses and broad-spectrum immune responses may confer cross-protection against heterologous influenza strains. In this study, we evaluated whether the immunogenic and protective potential of PCEP-adjuvanted inactivated swine influenza virus H1N1 vaccine can protect pigs immunized against live H3N2 virus. Piglets were vaccinated via the intradermal route with PCEP-adjuvanted inactivated swine influenza virus (SIV) H1N1 vaccine, boosted at day 21 with the same vaccines then challenged with infectious SIV H3N2 virus at day 35 via the tracheobronchial route. The pigs showed significant anti-H1N1 SIV specific antibody titres and H1N1 SIV neutralizing antibody titres, and these serum titres remained after the challenge with the H3N2 virus. In contrast, vaccination with anti-H1N1 SIV did not trigger anti-H3N2 SIV antibody titres or neutralizing antibody titres and these titres remained low until pigs were challenged with H3N2 SIV. At necropsy (six days after challenge), we collected prescapular lymph nodes and tracheobronchial draining the vaccination sites and challenge site, respectively. ELISPOTs from lymph node cells restimulated ex vivo with inactivated SIV H1N1 showed significant production of IFN-γ in the tracheobronchial cells, but not the prescapular lymph nodes. In contrast, lymph node cells restimulated ex vivo with inactivated SIV H1N1 showed significantly higher IL-13 and IL-17A in the prescapular lymph nodes draining the vaccination sites relative to unchallenged animals. Lung lesion scores show that intradermal vaccination with H1N1 SIV plus PCEP did not prevent lesions when the animals were challenged with H3N2. These results confirm previous findings that PCEP is effective as a vaccine adjuvant in that it induces strong immune responses and protects against homologous swine influenza H1N1 virus, but the experimental H1N1 vaccine failed to cross-protect against heterologous H3N2 virus.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Experimental PCEP-Adjuvanted Swine Influenza H1N1 Vaccine Induced Strong Immune Responses but Did Not Protect Piglets against Heterologous H3N2 Virus Challenge

et al. 2020

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Prospects for developing an Hepatitis C virus E1E2‐based nanoparticle vaccine

Toth

Andrianov

Fuerst

2023

Reviews in Medical Virology

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Globally, more than 58 million people are chronically infected with Hepatitis C virus (HCV) with 1.5 million new infections occurring each year. An effective vaccine for HCV is therefore a major unmet medical and public health need. Since HCV rapidly accumulates mutations, vaccines must elicit the production of broadly neutralising antibodies (bnAbs) in a reproducible fashion. Decades of research have generated a number of HCV vaccine candidates. Based on the available data and research through clinical development, a vaccine antigen based on the E1E2 glycoprotein complex appears to be the best choice, but robust induction of humoral and cellular responses leading to virus neutralisation has not yet been achieved. One issue that has arisen in developing an HCV vaccine (and many other vaccines as well) is the platform used for antigen delivery. The majority of viral vaccine trials have employed subunit vaccines. However, subunit vaccines often have limited immunogenicity, as seen for HCV, and thus multiple formats must be examined in order to elicit a robust anti‐HCV immune response. Nanoparticle vaccines are gaining prominence in the field due to their ability to facilitate a controlled multivalent presentation and trafficking to lymph nodes, where they can interact with both arms of the immune system. This review discusses the potential for development of a nanoparticle‐based HCV E1E2 vaccine, with an emphasis on the potential benefits of such an approach along with the major challenges facing the incorporation of E1E2 into nanoparticulate delivery systems and how those challenges can be addressed.

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Adjuvants: Engineering Protective Immune Responses in Human and Veterinary Vaccines

et al. 2021

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Adjuvants are key components of many vaccines, used to enhance the level and breadth of the immune response to a target antigen, thereby enhancing protection from the associated disease. In recent years, advances in our understanding of the innate and adaptive immune systems have allowed for the development of a number of novel adjuvants with differing mechanisms of action. Herein, we review adjuvants currently approved for human and veterinary use, describing their use and proposed mechanisms of action. In addition, we will discuss additional promising adjuvants currently undergoing preclinical and/or clinical testing.

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Intradermal immunization with inactivated swine influenza virus and adjuvant polydi(sodium carboxylatoethylphenoxy)phosphazene (PCEP) induced humoral and cell-mediated immunity and reduced lung viral titres in pigs

Cited by 16 publications

References 29 publications

Experimental PCEP-Adjuvanted Swine Influenza H1N1 Vaccine Induced Strong Immune Responses but Did Not Protect Piglets against Heterologous H3N2 Virus Challenge

Experimental PCEP-Adjuvanted Swine Influenza H1N1 Vaccine Induced Strong Immune Responses but Did Not Protect Piglets against Heterologous H3N2 Virus Challenge

Prospects for developing an Hepatitis C virus E1E2‐based nanoparticle vaccine

Adjuvants: Engineering Protective Immune Responses in Human and Veterinary Vaccines

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