Intradermal challenge with interleukin‐8 causes tissue oedema and neutrophil accumulation in atopic and non‐atopic human subjects

Douglass, Jo A; Dhami, D.; Bulpitt, M.; Lindley, I. J. D.; Shute, Janis K.; Church, Martin K.; Holgate, S. T.

doi:10.1111/j.1365-2222.1996.tb00538.x

Cited by 11 publications

(5 citation statements)

References 38 publications

(43 reference statements)

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“…However, at the site 1 cm away from allergen injection, there was little staining for adhesion molecules and no inflammatory cell accumulation. This was somewhat of a surprise considering that the levels of IL-6 and IL-8, both of which have been reported to increase adhesion molecule expression and inflammatory cell accumulation (Borish et al, 1989;Douglass et al, 1996), were similar to those recovered from the site of allergen injection. Further, while endothelial cells are good producers of the NF-kB-stimulated cytokines, IL-6 and IL-8, the generation of these cytokines is often accompanied by other NF-kB-stimulated products, including the adhesion molecules ICAM-1 and VCAM-1 (Lin et al, 2005).…”

Section: Discussionmentioning

confidence: 86%

What Can Microdialysis Tell Us About the Temporal and Spatial Generation of Cytokines in Allergen-Induced Responses in Human Skin In Vivo?

Clough

Jackson

Lee

et al. 2007

Journal of Investigative Dermatology

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This study examined the suitability of microdialysis to assess the time course of cytokine generation from discrete sites within the skin following intradermal injection of allergen. Cytokines were recovered using two microdialysis probes, one close to the point of allergen injection and the other 1 cm away but within the area of the late-phase induration. Skin biopsies taken at both sites were stained immunocytochemically to investigate possible relationships between cytokine generation, expression of adhesion molecules, and recruitment of neutrophils and eosinophils during the late-phase allergic response. The cytokine response to probe insertion was assessed using a single probe in the opposite arm (control). At baseline, microdialysate contained low levels of IL-1alpha, IL-5, IL-8, IL-12, GM-CSF, and TNFalpha (n=27-33). At control sites, this was followed by increases in IL-6 and IL-8 at 3 and 6 hours. Allergen increased TNFalpha levels in 3/11 individuals within 30 minutes at the injection site. Levels of IL-6 and IL-8 rose rapidly and were significantly greater (P<0.05) than that of controls at 3 and 6 hours at both injection and distant sites. Adhesion molecule expression and leukocyte infiltration were elevated only at the allergen injection site, suggesting a complex relationship between cytokine generation and cellular events in allergic inflammation. In conclusion, microdialysis can be used to distinguish temporal and spatial changes in protein profiles in the skin. Furthermore, when used in conjunction with skin biopsies, it provides novel information about the mechanisms of dermal inflammation.

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Section: Discussionmentioning

confidence: 86%

What Can Microdialysis Tell Us About the Temporal and Spatial Generation of Cytokines in Allergen-Induced Responses in Human Skin In Vivo?

Clough

Jackson

Lee

et al. 2007

Journal of Investigative Dermatology

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“…IL-8 is a chemokine that plays an important role in neutrophil inflammatory responses including upregulation of neutrophil expression of CD11b and shedding of L-selectin, steps necessary for firm adhesion to endothelial cells and movement into tissue (Hoch et al, 1996;Mukaida et al, 1998). In addition, IL-8 is expressed in tissue with neutrophilic infiltrates and injection of IL-8 into skin results in the accumulation of neutrophils (Lira et al, 1994;Douglass et al, 1996;Murphy, 1997). We previously demonstrated that neutrophils in patients with DH have elevated expression of CD11b, a decreased expression of L-selectin and increased function of neutrophil Fc IgA receptor (Smith et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Serum IL-8 in Patients with Dermatitis Herpetiformis is Produced in Response to Dietary Gluten

Hall

Benbenisty

Mickle

et al. 2007

Journal of Investigative Dermatology

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Patients with dermatitis herpetiformis (DH) have a gluten-sensitive enteropathy and while on gluten-containing diets have elevated levels of serum IL-8. We hypothesized that the mucosal immune response to gluten is responsible for the elevated serum IL-8. Six DH patients were studied while on a gluten-free diet (GFD), whereas four continued on a normal diet. Patients were followed for a mean 2.2 years and serum IL-8 was analyzed. Small bowel biopsies from five DH patients on normal diets, two DH patients on GFD, and six subjects with no small bowel abnormalities were analyzed for IL-8 mRNA. Serum IL-8 levels normalized in five of six patients on GFD and decreased in one, whereas serum IL-8 levels showed no statistically significant change in DH patients on normal diets. Small bowel biopsies from DH patients on normal diets had increased expression of IL-8 mRNA compared to normal subjects, whereas patients on a GFD showed no significant increase in small bowel mRNA. No significant IL-8 mRNA was detected in normal skin biopsies from patients with DH. These observations suggest that the IL-8 in the serum of patients with DH originates from the small bowel as a mucosal immune response to gluten ingestion.

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“…Lifespan and size of large animals are more similar to men than to rodents, as is their developmental, innate, and mucosal immunity. For instance, mice lack the gene encoding for the interleukin-8 (Hol et al, 2010), a cytokine implicated in severe neutrophilic asthma and in respiratory virus-induced asthma exacerbations (Nakagome et al, 2012;Rohde et al, 2014), and also essential for neutrophil recruitment in men, cattle, and horses (Caswell et al, 1999;Caswell et al, 2001;Cook et al, 2009;Douglass et al, 1996;Franchini et al, 1998;Kaur and Singh, 2013).…”

Section: Development Of Animal Models Better Mimicking Human Diseasesmentioning

confidence: 99%

Asthma “of horses and men”—How can equine heaves help us better understand human asthma immunopathology and its functional consequences?

Bullone

Lavoie

2015

Molecular Immunology

128

142

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Animal models have been studied to unravel etiological, immunopathological, and genetic attributes leading to asthma. However, while experiments in which the disease is artificially induced have helped discovering biological and molecular pathways leading to allergic airway inflammation, their contribution to the understanding of the causality of the disease has been more limited. Horses naturally suffer from an asthma-like condition called "heaves" which presents sticking similarities with human asthma. It is characterized by reversible airway obstruction, airway neutrophilic inflammation, and a predominant Th2 immune response. This model allows one to investigate the role of neutrophils in asthma, which remains contentious, the regulation of chronic neutrophilic inflammation, and their possible implication in pulmonary allergic responses. Furthermore, the pulmonary remodeling features in heaves closely resemble those of human asthma, which makes this model unique to investigate the kinetics, reversibility, as well as the physiological consequences of tissue remodeling. In conclusion, heaves and asthma share common clinical presentation and also important immunological and tissue remodeling features. This makes heaves an ideal model for the discovery of novel pathways implicated in the asthmatic inflammation and associated tissue remodeling.

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Intradermal challenge with interleukin‐8 causes tissue oedema and neutrophil accumulation in atopic and non‐atopic human subjects

Abstract: This study demonstrates that in human skin, IL-8 induces increased microvascular permeability and neutrophil infiltration, but not eosinophil or T-lymphocyte chemoattraction.

Cited by 11 publications

References 38 publications

What Can Microdialysis Tell Us About the Temporal and Spatial Generation of Cytokines in Allergen-Induced Responses in Human Skin In Vivo?

What Can Microdialysis Tell Us About the Temporal and Spatial Generation of Cytokines in Allergen-Induced Responses in Human Skin In Vivo?

Serum IL-8 in Patients with Dermatitis Herpetiformis is Produced in Response to Dietary Gluten

Asthma “of horses and men”—How can equine heaves help us better understand human asthma immunopathology and its functional consequences?

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