Intracytoplasmic sperm injection with cryopreserved testicular spermatozoa

Effect of sperm storage and selection techniques on sperm parameters

“…These droplets can then be introduced into LN 2 for storage. In one study, this method of storing sperm produced a clinical pregnancy, and implantation rates were similar to those of fresh testicular samples [Gil-Salom et al 2000].…”

Cryopreservation of Human Spermatozoa by Vitrification vs. Slow Freezing: Canadian Experience

“…ICSI pipettes were suggested as a container to freeze individual spermatozoa by either the slow method or vitrification (AbdelHafez et al, 2009;Sohn et al, 2003). Cryopreservation of sperm in microdroplets containing 1 or 40 μl of spermatozoa in cryoprotectant placed on a cold surface or directly plunged into liquid nitrogen was also reported (Gil-Salom et al, 2000;Isachenko et al, 2005). Microdroplets covered by mineral oil in a plastic tissue culture dish placed in liquid nitrogen were also used to cryopreserve individual spermatozoa (Quintans et al, 2000;Sereni et al, 2008).…”

Genome Banking for Vertebrates Wildlife Conservation

“…When the initial sample is of very poor quality or with very small number of cells, small cell-number or single cell cryopreservation techniques may become useful. Starting in the late 1990's , reports on several single sperm cryopreservation techniques showed up in the scientific literature (Walmsley et al, 1998;Gil-Salom et al, 2000;Gvakharia & Adamson, 2001;Just et al, 2004;Herrler et al, 2006;Isaev et al, 2007;Koscinski et al, 2007;Woods et al, 2010). Using these various techniques, researchers reported a wide range of outcomes and recovery efficiency.…”