Intracytoplasmic morphologically selected sperm injection (IMSI) after repeated IVF or ICSI failures: a prospective comparative study

Delaroche, Lucie; Yazbeck, Chadi; Gout, C.; Kahn, Vanessa; Oger, Pierre; Rougier, Nathalie

doi:10.1016/j.ejogrb.2012.11.011

Cited by 31 publications

(16 citation statements)

References 30 publications

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“…Use of higher magnification approaches, such as intracytoplasmic morphological sperm injection (IMSI) do not appear overly beneficial, though this may depend on patient population. [15][16][17][18] Emerging selection approaches, such as those use hyaluronan binding to identify/select sperm, may also be useful, 19 though this likely depends on the patient population and semen characteristics. 20…”

Section: Gamete Processingmentioning

confidence: 99%

Optimal Human Embryo Culture

Swain

2015

Semin Reprod Med

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A large contributor to success during in vitro fertilization (IVF) lies in the processes occurring within the IVF laboratory. These processes make up the "culture system." This system entails numerous procedures and technical steps that must be optimized to produce a competent embryo. Notably, variations exist between programs that include differences in patient population, clinical stimulation, and other factors. Thus, a single "optimal" culture system to be utilized between all laboratories is likely not feasible. Rather, laboratory procedures should be optimized based on an individual laboratory's performance. That being said, within the scientific literature, there are key components, approaches, and techniques within the culture system that have been shown to be superior to alternatives. These key components important in improving embryo culture are discussed.

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Section: Gamete Processingmentioning

confidence: 99%

Optimal Human Embryo Culture

Swain

2015

Semin Reprod Med

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“…Thus, after two or three IVF/ICSI failures, IMSI seemed to give better embryo quality and more blastocysts, which allowed more ETs at the blastocyst stage. This study supported the use of sperm ultramorphology examination as an independent test to be proposed after repeated IVF or ICSI failures [24].…”

Section: Discussionmentioning

confidence: 78%

“…It is, however, a complicated technique that cannot be routinely performed [24]. Values were expressed as mean ± SD for parametric data or as median (minimum-maximum) for nonparametric data.…”

Section: Discussionmentioning

confidence: 99%

Will intracytoplasmic morphologically selected sperm injection replace intracytoplasmic sperm injection in cases of severe oligo-astheno-teratozoospermia?

Orief

Younan

El-Agwany

2016

Evidence Based Womenʼs Health Journal

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ObjectiveThe aim of the study was to compare between intracytoplasmic morphologically selected sperm injection (IMSI) and conventional intracytoplasmic sperm injection (ICSI) as regards laboratory and clinical outcomes in couples with severe oligoastheno-teratozoospermia (OAT). Design This was a prospective, nonrandomized, controlled clinical trial. SettingThe study was conducted in the Agial ICSI/IVF Center. Materials and methods A total of 1729 couples with severe OAT were enrolled: 1356 couples were submitted to routine ICSI (group I) and 373 couples were submitted to IMSI (group II) after they had been counselled about the possible role of the IMSI technique in increasing pregnancy rates. For IMSI, spermatozoa were selected at 6600 Â magnification using an inverted microscope. For conventional ICSI, spermatozoa were selected at 400 Â magnification. Main outcome measures Laboratory (fertilization rate, embryo quality, number of embryos transferred) and clinical outcomes (clinical pregnancy rate) were compared between the two groups. Both groups were comparable regarding the number of mature (MII) oocytes collected (P = 0.36) and the total number of embryos transferred (P = 0.202). No statistically significant difference was observed between the two groups regarding rates of fertilization (P = 0.946), with a mean ± SD of 63.47 ± 25.15% in group I and 64.69 ± 22.66% in group II. However, a statistically significant difference (Po0.001) was present regarding clinical pregnancy rates, 44 versus 57% in groups I and II, respectively. There was a lower number of cycles with no embryo transfer in the IMSI group versus the ICSI group (4.3 vs. 6.7%), but the difference was statistically insignificant (P = 0.086). Major conclusionOur results suggest that IMSI provides a significant improvement in clinical pregnancy rates compared with ICSI in couples with severe OAT.

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“…Numerous studies have attempted to compare standard ICSI to IMSI with varying results. Several studies show improvement in healthy fertilizations when implementing IMSI, especially for couples who have undergone and experienced repeated ICSI failure [9][10][11][12], while other studies indicate that IMSI does not produce any significant increase in successful fertilizations and implantations [13,14].…”

Section: Intracytoplasmic Morphologically Selected Sperm Injection (Imentioning

confidence: 99%

Label-Free Quantitative Imaging of Sperm for In-Vitro Fertilization Using Interferometric Phase MicroscopyIVF, IMSI, ICSI, IPM, Holography, Imaging, Sperm

Mirsky¹,

Barnea²,

Shaked³

2016

JFIV Reprod Med Genet

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Accurate morphological evaluation via imaging of sperm cells is a critical step in both routine sperm analysis and sperm cell selection for in vitro fertilization. In this paper, we review the significant advantages of interferometric phase microscopy (IPM), also called digital holographic microscopy, for label-free sperm imaging in fertility clinics. We first review the current state of the art of label-free sperm cell imaging for in vitro fertilization, including brightfield, Zernike phase contrast, differential interference contrast (DIC), Hoffman modulation contrast and polarized light microscopy, as well as intracytoplasmic morphologically selected sperm injection (IMSI). Using experimental demonstrations, we compare the limitations of these techniques for label-free sperm imaging. IPM and its advantages are then discussed in detail. These include the ability to receive quantitative data regarding the 3D structure of sperm cells, as well as data concerning sperm biomass and morphology without labeling. The current gap to clinical use is discussed, as well as the possible solution of employing compact and simple holographic modules, which may allow holographic imaging to reach the clinic in the near future.

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Intracytoplasmic morphologically selected sperm injection (IMSI) after repeated IVF or ICSI failures: a prospective comparative study

Cited by 31 publications

References 30 publications

Optimal Human Embryo Culture

Optimal Human Embryo Culture

Will intracytoplasmic morphologically selected sperm injection replace intracytoplasmic sperm injection in cases of severe oligo-astheno-teratozoospermia?

Label-Free Quantitative Imaging of Sperm for In-Vitro Fertilization Using Interferometric Phase MicroscopyIVF, IMSI, ICSI, IPM, Holography, Imaging, Sperm

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