Intracrystalline incorporation of nacre protein hydrogels modifies the mechanical properties of calcite crystals: a microcompression study

Risan, Jared; Jain, Gaurav; Pendola, Martin; Evans, John Spencer

doi:10.1039/c8tb01156g

Cited by 6 publications

(13 citation statements)

References 34 publications

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“…The transcriptome or RNA expression levels in these organisms are also being explored with the goal of understanding the timing of transcription, translation, and protein secretion as it pertains to the events occurring in the biomineralizing matrix . What has emerged from these studies…”

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

“…This framework coating provides a number of survival strategies to each layer. In the case of the nacre layer, these include ductility, elasticity, and fracture resistance; in the case of the prismatic layer, these are organized prismatic collapse and puncture‐resistance In contrast, the intracrystalline secretomes are families of proteins that exist within the interiors of calcite or aragonite single crystals . These proteins form nanoinclusions within the mineral phases of each layer, creating protein gel–induced nanoporosities which lighten and strengthen the crystal …”

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

“…Many of these proteins, such as AP7, AP24, aspein, asprich, perlucin, calprismin, caspartin, and AP8 have pIs < 7 and are considered “acidic” or polyelectrolyte in nature due to their high content of Asp, Glu, and in some cases the presence of anionic oligosaccharides . It is suspected that these anionic proteins attract Ca(II) and water and regulate the nucleation process, for example, by controlling the formation, stabilization, and transformation of ACC to crystalline calcium carbonates, and/or by binding to forming crystals and regulating their growth . Like their framework counterparts, intracrystalline proteins sequences are a combination of intrinsically disordered regions mated with conserved protein–protein interactive domains (Figure ), but lack the chitin‐binding sequence found in framework proteins .…”

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

“…It is suspected that these anionic proteins attract Ca(II) and water and regulate the nucleation process, for example, by controlling the formation, stabilization, and transformation of ACC to crystalline calcium carbonates, and/or by binding to forming crystals and regulating their growth . Like their framework counterparts, intracrystalline proteins sequences are a combination of intrinsically disordered regions mated with conserved protein–protein interactive domains (Figure ), but lack the chitin‐binding sequence found in framework proteins . Intracrystalline proteins are also known to form hydrogel particles that become incorporated into forming crystals, thereby creating nanoinclusions of protein hydrogels that make the crystal more ductile and fracture resistant and thus augmenting the fracture‐resistance offered by the framework proteome.…”

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

“…Like their framework counterparts, intracrystalline proteins sequences are a combination of intrinsically disordered regions mated with conserved protein–protein interactive domains (Figure ), but lack the chitin‐binding sequence found in framework proteins . Intracrystalline proteins are also known to form hydrogel particles that become incorporated into forming crystals, thereby creating nanoinclusions of protein hydrogels that make the crystal more ductile and fracture resistant and thus augmenting the fracture‐resistance offered by the framework proteome.…”

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

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The Biomineralization Proteome: Protein Complexity for a Complex Bioceramic Assembly Process

Evans

2019

Proteomics

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There are over 62 different biominerals on Earth and a diverse array of organisms that generate these biominerals for survival. This review will introduce the process of biomineralization and the current understanding of the molecular mechanisms of mineral formation, and then comparatively explore the representative secretomes of two well-documented skeletal systems: vertebrate bone (calcium phosphate) and invertebrate mollusk shell (calcium carbonate). It is found that both skeletal secretomes have gross similarities and possess proteins that fall into four functional categories: matrix formers, nucleation assisters, communicators, and remodelers. In many cases the mineral-associated matrix former and nucleation assister sequences in both skeletal systems are unique and possess interactive conserved globular domains, intrinsic disorder, post-translational modifications, sequence redundancy, and amyloid-like aggregation-prone sequences. Together, these molecular features create a protein-based environment that facilitates mineral formation and organization and argue in favor of conserved features that evolve from the mollusk shell to bone. Interestingly, the mollusk shell secretome appears to be more complex compared to that of bone tissue, in that there are numerous protein subcategories that are required for the nucleation and organization of inner (nacre) and outer (prismatic) calcium carbonate regions of the shell. This may reflect the organizational and material requirements of an exoskeletal protective system.

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Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

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The Biomineralization Proteome: Protein Complexity for a Complex Bioceramic Assembly Process

Evans

2019

Proteomics

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Multi‐Functionalization of Single crystals Mediated by Gel‐Incorporation: A Bioinspired Strategy

Yao,

Ren,

2023

ChemPlusChem

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In nature, the biominerals are always organic‐inorganic crystal composites. Inspiring by this biomineral structure, the functionalized single crystal can be synthesized by the gel‐grown method, which also leads to the incorporation of gel‐networks into the crystal. The gel‐incorporation expands the variety of functionalized substances that can be incorporated into crystals, such as the nanoparticles without surface modification. And the gel‐incorporation promotes the dye or fluorescent molecules to be isotopically incorporated into crystals. Besides, the gel‐incorporation also enhances the toughness and stability of crystals, even endowing protein crystals with the self‐healing property. More importantly, the recent research indicated that the incorporation of the gel‐networks enhances their charge effects on the crystal morphology control. In general, the gel‐incorporation not only expands the scope of functionalization of crystal materials, but also extends the application scenario. Therefore, preparing the single crystal with gel‐incorporation is a remarkable strategy for synthesizing functionalized crystals with the better mechanical property and controllable morphology.

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Tensile and compressive mechanical properties of nanocrystalline calcite with grain size effect

Luo,

Yang,

2023

Journal of Mechanics

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Calcite is one of the most main components of microbially induced calcium carbonate precipitation (MICP). With the in-depth research of MICP, the mechanical properties of nanocrystalline calcite attract much attention. In this paper, the deformation and failure behaviors of nanocrystalline calcite under a uniaxially tensile or compressive condition are studied by molecular dynamics simulation, and then the dominant deformation and failure mechanisms, as well as their grain size effect, are analyzed. The results show that the grain boundary densification dominates the elastic deformation while the intragranular phase transition dominates the plastic deformation. Compared with single-crystal calcite, the elastic modulus of nanocrystalline calcite is significantly reduced and its ultimate strength is decreased by more than 50%. Nanocrystalline calcite has stronger plastic deformation ability in compression than in tension. Its tensile and compressive elastic modulii and peak stresses all increase with the average grain size. The effects of grain size on the limit stresses can be described by the inverse Hall-Petch equation. This study is helpful for tailoring the mechanical properties of MICP by the morphology of nanocrystalline calcite.

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Intracrystalline incorporation of nacre protein hydrogels modifies the mechanical properties of calcite crystals: a microcompression study

Abstract: The fracture toughness of mollusk shell nacre has been attributed to many factors, one of which is the intracrystalline incorporation of nacre-specific proteins.

Cited by 6 publications

References 34 publications

The Biomineralization Proteome: Protein Complexity for a Complex Bioceramic Assembly Process

The Biomineralization Proteome: Protein Complexity for a Complex Bioceramic Assembly Process

Multi‐Functionalization of Single crystals Mediated by Gel‐Incorporation: A Bioinspired Strategy

Tensile and compressive mechanical properties of nanocrystalline calcite with grain size effect

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