Intracoronary delivery of bone-marrow-derived stem cells

Bui, Quang T.; Gertz, Zachary M.; Wilensky, Robert L.

doi:10.1186/scrt29

Cited by 29 publications

(19 citation statements)

References 45 publications

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“…In fact, the meager number of progenitor cells homing and integrating into the myocardial architecture after conventional procedures is not determined by inherent myocardial impedimenta [39], but by the lack of at least two irremissible factors: proper culture of optimal progenitor cells and most favorable host tissue environment. Consistently, the present study was designed to verify the capability of properly cultured progenitor cells to home in a favorable host tissue environment (healthy myocardium).…”

Section: Discussionmentioning

confidence: 99%

Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts

et al. 2011

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Human heart harbors a population of resident progenitor cells that can be isolated by stem cell antigen-1 antibody and expanded in culture. These cells can differentiate into cardiomyocytes in vitro and contribute to cardiac regeneration in vivo. However, when directly injected as single cell suspension, less than 1%-5% survive and differentiate. Among the major causes of this failure are the distressing protocols used to culture in vitro and implant progenitor cells into damaged hearts. Human cardiac progenitors obtained from the auricles of patients were cultured as scaffoldless engineered tissues fabricated using temperature-responsive surfaces. In the engineered tissue, progenitor cells established proper three-dimensional intercellular relationships and were embedded in self-produced extracellular matrix preserving their phenotype and multipotency in the absence of significant apoptosis. After engineered tissues were leant on visceral pericardium, a number of cells migrated into the murine myocardium and in the vascular walls, where they integrated in the respective textures. The study demonstrates the suitability of such an approach to deliver stem cells to the myocardium. Interestingly, the successful delivery of cells in murine healthy hearts suggests that myocardium displays a continued cell cupidity that is strictly regulated by the limited release of progenitor cells by the adopted source. When an unregulated cell source is added to the system, cells are delivered to the myocardium. The exploitation of this novel concept may pave the way to the setup of new protocols in cardiac cell therapy.

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Section: Discussionmentioning

confidence: 99%

Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts

et al. 2011

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“…Intracoronary infusion is another option to deliver cells into the myocardial region of injury. While this approach can minimize cell leakage and may limit their systemic diffusion, injected cells may not be retained well (16) and can cause acute myocardial ischemia and myocardial microinfarction (143). Cells may be delivered via a homing system, which enables endogenous or exogenous cells to migrate to a specific region.…”

Section: Cell Delivery and Survivalmentioning

confidence: 99%

Regenerative Medicine for the Heart: Perspectives on Stem-Cell Therapy

Cho

Fernandez

Kwon

2014

Antioxidants & Redox Signaling

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Significance: Despite decades of progress in cardiovascular biology and medicine, heart disease remains the leading cause of death, and there is no cure for the failing heart. Since heart failure is mostly caused by loss or dysfunction of cardiomyocytes (CMs), replacing dead or damaged CMs with new CMs might be an ideal way to reverse the disease. However, the adult heart is composed mainly of terminally differentiated CMs that have no significant self-regeneration capacity. Recent Advances: Stem cells have tremendous regenerative potential and, thus, current cardiac regenerative research has focused on developing stem cell sources to repair damaged myocardium. Critical Issues: In this review, we examine the potential sources of cells that could be used for heart therapies, including embryonic stem cells and induced pluripotent stem cells, as well as alternative methods for activating the endogenous regenerative mechanisms of the heart via transdifferentiation and cell reprogramming. We also discuss the current state of knowledge of cell purification, delivery, and retention. Future Directions: Efforts are underway to improve the current stem cell strategies and methodologies, which will accelerate the development of innovative stem-cell therapies for heart regeneration.

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“…Results obtained in pre-clinical animal models showed that this minimally invasive approach results in a significant percentage of injected cells being sequestered in lungs, liver or spleen, due to blood flow [33]. On the other hand, intracoronary or retrograde coronary sinus infusion of the cells are mainly performed after acute myocardial infarction using an angioplasty balloon and high pressure to deliver cells to heart muscle [34]. The coronary route was proven to be free of stem cell systemic delivery, while a limited number of cells could be found in the infarcted area [35].…”

Section: A) Direct Injectionmentioning

confidence: 99%