Intracompartmental Pressure as a Predictor of Intratesticular Blood Flow: A Rat Model

Watson, Matthew J.; Bartkowski, Donald P.; Nelson, Nathan C.

doi:10.1016/j.juro.2014.12.018

Cited by 8 publications

(3 citation statements)

References 17 publications

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“…Though local injection can bypass the blood-testis barrier and directly affect the testis, it is not a good treatment option in clinical practice. The potential risk of compartment syndrome by local injection has limited its clinical application [ 11 , 12 ]. In our previous study of I/R injury of the kidney, intravenous stem cells still conducted a great protective effect though they were blocked in the lung [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…A study reported that MSCs transplanted by local injection into I/R injured testis played a protective effect on germ cells [ 10 ]. However, local injection of MSCs may aggravate the intracompartmental pressure of testis, resulting in an occlusion of the microvascular bed that feeds the testicular lobules [ 11 , 12 ]. On the other hand, intravenous injection might be an appropriate transplantation method for MSCs, which can avoid these risks.…”

Section: Introductionmentioning

confidence: 99%

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Human umbilical cord multipotent mesenchymal stromal cells alleviate acute ischemia-reperfusion injury of spermatogenic cells via reducing inflammatory response and oxidative stress

et al. 2020

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Background This study was designed to determine the effect of human umbilical cord multipotent mesenchymal stromal cells (hUC-MSC) on acute ischemia/reperfusion (I/R) injury of spermatogenic cells. Method The testicular I/R rat model was established through 720° torsion for 1 h. hUC-MSC were intravenously injected 10 min before detorsion. Injury severity of spermatogenic cells was estimated by Johnsen’s score. The proliferating of recipient spermatogonia was measured by the immunostaining of antibodies against Ki67, and all germ cells were detected with DDX4 antibody. And recipient spermatogenesis was assessed by staining spermatozoa with lectin PNA. The levels of inflammatory factors were measured by real-time PCR. And the Selectin-E expression, neutrophil infiltration in the testes was detected by immunostaining. Germ cells apoptosis was tested by TUNEL assay and western blot. Furthermore, the oxidative stress was tested by reactive oxidative species (ROS) levels. In vitro, the condition medium (CM) of hUC-MSC was used to culture human umbilical vein endothelial cells (HUVECs), so as to assess the paracrine effect of hUC-MSC on HUVECs. The protein chip was used to measure the relative concentration of the secretory proteins in the CM of hUC-MSC. Result hUC-MSC greatly alleviated the testicular injury induced by testis I/R. The levels of proinflammatory factors were downregulated by hUC-MSC in vivo and in vitro. Neutrophil infiltration, ROS, and germ cell apoptosis in testicular tissues were greatly reduced in the group of hUC-MSC. Paracrine factors secreted by hUC-MSC including growth factors, cytokines, and anti-inflammatory cytokine were rich. Conclusion This study demonstrated that intravenously injected hUC-MSC could protect the spermatogenic cells against I/R injury by reducing the inflammatory response, apoptosis, and acute oxidative injury. Paracrine mechanism of hUC-MSC may contribute to the protection of spermatogenic cells against I/R injury. Therefore, the present study provides a method for clinical treatment of attenuate I/R injury of spermatogenic cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human umbilical cord multipotent mesenchymal stromal cells alleviate acute ischemia-reperfusion injury of spermatogenic cells via reducing inflammatory response and oxidative stress

et al. 2020

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“…4 Intraparenchymal monitoring of testicular pressures has also shown how complex the reperfusion process can be after detorsion and the development of a compartment syndrome. [4][5][6] This intraoperative method is invasive and technically complex, which makes it not accessible and not reproducible in each and every case. To date, there is no objective method to assess testicular reperfusion that can be used for intraoperative decision making.…”

Section: Introductionmentioning

confidence: 99%

Thermographic Patterns for Real-time Intraoperative Monitoring of Testicular Reperfusion Following Surgical Testicular Detorsion

et al. 2018

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A 5-year-old patient presents to the emergency department with testicular torsion. Intraoperative sequential infrared thermographic images were registered in real-time using a FLIR One (FLIR Systems, Inc., Wilsonville, OR, US) infrared camera. The temperatures of the scrotum and the testis prior to scrotal exploration were 43.6°C on the affected side, and 41.7°C on the contralateral side. The core temperature of the patient was 36.8°C. Immediately after the tunica vaginalis had been opened and the testis had been brought out through the incision, the temperature of the testicle was 31.5°C. After 30 seconds of being detorted, the temperature increased to 34.3°C. The use of intraoperative infrared thermographic imaging helps to detect discrete changes in testicular temperature, which suggests it may be useful to objectively assess reperfusion. Future studies will require more patients to correlate postoperative blood flow to the injured testis as well as volume changes after surgery to see if the intraoperative thermography findings can be used as a predictive tool for postoperative outcomes. ResumenPaciente de 5 años de edad con historia de 8 horas de torsión testicular es llevado a cirugía. Se realiza registro imaginonlógico termográfico seriado en tiempo real del momento en que se realiza la destorsión utilizando una cámara infrarroja FLIR One. La temperatura escrotal previa a la cirgía era de 43,6°C del lado afectado y 41,7°C del lado sano. La temperatura corporal del paciente era de 36,8°C. Inmediatemente el testiculo fue extraído y previo a la destorsión, la temperatura era de 31,5°C. A los 30 segundos de

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Basic Research Applied to Testicular Torsion

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et al. 2020

Translational Research in Pediatric Urology

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Intracompartmental Pressure as a Predictor of Intratesticular Blood Flow: A Rat Model

Abstract: This is the first known study to characterize a stop flow pressure within the testicular parenchyma resulting from an increased intracompartmental pressure. Due to probe sensitivity limitations, color flow appears to provide the most precise mean pressure of occlusion of 52.17 mm Hg.

Cited by 8 publications

References 17 publications

Human umbilical cord multipotent mesenchymal stromal cells alleviate acute ischemia-reperfusion injury of spermatogenic cells via reducing inflammatory response and oxidative stress

Human umbilical cord multipotent mesenchymal stromal cells alleviate acute ischemia-reperfusion injury of spermatogenic cells via reducing inflammatory response and oxidative stress

Thermographic Patterns for Real-time Intraoperative Monitoring of Testicular Reperfusion Following Surgical Testicular Detorsion

Basic Research Applied to Testicular Torsion

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