Intraclonal diversity of fibrosarcoma cells for the production of macrophage colony‐stimulating factor and granulocyte colony‐stimulating factor

Sakai, Nobuo; Kubota, M; Shikita, Mikio; Yokota, Masaru; Andō, Kōichi

doi:10.1002/jcp.1041330228

Cited by 15 publications

(4 citation statements)

References 12 publications

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“…Fibroblast cells and fibrosarcoma cell lines are a good source of M-CSF. Native M-CSF was purified from the conditioned media of these cell lines (41,42).…”

Section: Discussionmentioning

confidence: 99%

Constitutive production of macrophage colony-stimulating factor by human ovarian and breast cancer cell lines.

et al. 1989

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Many nonhematologic tumors produce growth factors that may influence cellular proliferation either by autocrine or by paracrine mechanisms. In the current study, human tumor cell lines were investigated for the constitutive production of macrophage colony-stimulating factor (M-CSF). Culture supernatants obtained from cell lines were analyzed using a radioimmunoassay and a radioreceptor assay specific for M-CSF. Among the various cell types analyzed, all the ovarian cell lines and a majority of the breast cancer cell lines secreted significant amount of an M-CSF-like factor. Treatment of mouse bone marrow cultures with culture supernatants from ovarian cancer cells stimulated the production of macrophage colonies. Analysis of total cellular RNA obtained from the ovarian cell lines by Northern blot showed multiple sizes of M-CSF transcripts with an abundance of a 4.2-kb message. The relative amount of M-CSF transcripts correlated with the level of immunoreactive material seen in the culture supernatants.

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“…Fibroblast cells and fibrosarcoma cell lines are a good source of M-CSF. Native M-CSF was purified from the conditioned media of these cell lines (41,42).…”

Section: Discussionmentioning

confidence: 99%

Constitutive production of macrophage colony-stimulating factor by human ovarian and breast cancer cell lines.

et al. 1989

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“…The production of granulocyte colony‐stimulating factor (G‐CSF) or granulocyte macrophage colony‐stimulating factor (GM‐CSF) by the tumor itself is thought to be responsible for this paraneoplastc syndrome 1, 2, 3. Varieties of nonhematopoietic malignant tumors, including bladder carcinoma,4, 5, 6 hepatoma,7 mesothelioma,8 squamous cell carcinoma of the oropharynx,9 melanoma,10 glioblastoma,11 sarcoma12,13 and carcinoma of the lung14, 15, 16, 17, 18 have been demonstrated to secrete G‐CSF or GM‐CSF in an amount large enough to cause a significant systemic hematopoietic effect. Several reports also have demonstrated the presence of an autocrine growth loop for G‐CSF and GM‐CSF in nonhematopoietic tumor cells 19, 20, 21.…”

mentioning

confidence: 99%

Effect of serum deprivation on constitutive production of granulocyte‐colony stimulating factor and granulocyte macrophage‐colony stimulating factor in lung cancer cells

Uemura

Kobayashi

Nakata

et al. 2004

Intl Journal of Cancer

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We previously established 2 lung cancer cell lines, OKa‐C‐1 and MI‐4, which constitutively produce an abundant dose of granulocyte‐colony stimulating factor (G‐CSF) and granulocyte macrophage‐colony stimulating factor (GM‐CSF). Many other cases with G‐CSF or GM‐CSF producing tumors have been reported up to the present. However, the biological properties of the overproduction of G‐CSF and GM‐CSF by tumor cells have not been well known. Several reports demonstrated the presence of an autocrine growth loop for G‐CSF and GM‐CSF in nonhematopoietic tumor cells. We showed that exogenous G‐CSF and GM‐CSF stimulated cell growth in a dose‐dependent manner in OKa‐C‐1 and MI‐4 cells. We could detect the presence of G‐CSF and GM‐CSF receptors in both cell lines by RT‐PCR analysis. We have previously shown that inflammatory cytokines, tumor necrosis factor (TNF)‐α and interleukin (IL)‐1β enhance the expression of G‐CSF and GM‐CSF in the cell lines. However, the factors that regulate constitutive production of G‐CSF or GM‐CSF by tumor cells are still unknown well. In our study, we first reported that serum deprivation stimulated constitutive production of G‐CSF and GM‐CSF by lung tumor cells through activation of nuclear factor (NF)‐κB and p44/42 mitogen‐activated protein kinase (MAPK) pathway signaling. We suggest that G‐CSF and GM‐CSF constitutively produced by tumor cells could grow tumor itself and rescue tumor cells from the cytotoxicity of serum deprivation. © 2004 Wiley‐Liss, Inc.

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“…The finding that a higher proportion of cells in the population contain GM-CSF transcripts compared to Multi-CSF transcripts suggests that within an individual cell, the genes may be differentially regulated. A similar approach may be used to elucidate the basis for the intraclonal heterogeneity in G-CSF and M-CSF production by fibrosarcoma cells (Sakai et al, 1987).…”

mentioning

confidence: 99%

Detection of colony‐stimulating factor messenger RNA in single T cells by in situ hybridization

Williamson

Owens

Pearse

1989

Journal Cellular Physiology

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In situ hybridization has been used to study the accumulation of colony-stimulating factor (CSF) mRNA in single cells of a T lymphocyte clone (E9.D4) following antibody-mediated (F23.1) activation via the Ti-T3 complex in filler-independent bulk cultures. The specificity of hybridization for cellular RNA was demonstrated by pretreating the cells with the Ca2+-dependent enzyme micrococcal nuclease by using a novel protocol developed for use with riboprobes. Maximal levels of granulocyte-macrophage (GM) and multipotential-CSF (interleukin 3) mRNA were detected after 8-10 h, with GM-CSF mRNA being detected earlier and at a lower concentration of stimulus. The rise in intracellular mRNA was accompanied by an increase in the corresponding CSF bioactivity in the supernatant. In situ hybridization was of comparable sensitivity to Northern blot analysis and revealed significant heterogeneity in the accumulation of CSF mRNA within individual cells of the clone following stimulation with F23.1. This could account for the corresponding heterogeneity in CSF production by single cells. Under optimal conditions at least 25% of cells contained both transcripts. The method has been used to examine CSF production by normal spleen cells and should be useful in the further analysis of lymphokine gene regulation in single T cells.

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Intraclonal diversity of fibrosarcoma cells for the production of macrophage colony‐stimulating factor and granulocyte colony‐stimulating factor

Cited by 15 publications

References 12 publications

Constitutive production of macrophage colony-stimulating factor by human ovarian and breast cancer cell lines.

Constitutive production of macrophage colony-stimulating factor by human ovarian and breast cancer cell lines.

Effect of serum deprivation on constitutive production of granulocyte‐colony stimulating factor and granulocyte macrophage‐colony stimulating factor in lung cancer cells

Detection of colony‐stimulating factor messenger RNA in single T cells by in situ hybridization

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