Intracerebroventricular injection of orexins does not stimulate food intake in neonatal chicks

Furuse, Mitsuhiro; Ando, Ryuichi; Bungo, Takashi; Shimojo, Masako; Masuda, Yuichi

doi:10.1080/00071669987115

Cited by 216 publications

(71 citation statements)

References 9 publications

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“…In teleost fish, central injection of human orexin peptides can stimulate food intake in goldfish (Volkoff et al 1999, Volkoff & Peter 2001. Contrary to results demonstrated in other species, human orexins did not stimulate food intake in neonatal chicks (Furuse et al 1999). In addition, we recently cloned prepro-orexin cDNA from chicken hypothalamus and described that food deprivation for 24 h did not alter orexin mRNA expression in the chicken hypothalamus (Ohkubo et al 2002).…”

Section: Atacatcgtcgtgttcatcgtggctctcgtcgggaacgtgctggtttgtattgcagtgtgmentioning

confidence: 99%

“…Furthermore, there is no available report describing cDNA cloning for OXR(s) in non-mammalian species, and the tissue distribution of the receptor would be invaluable for gaining insight into the physiological actions of orexin in non-mammalian vertebrates. At least in birds, data suggest that orexin is not involved in the regulation of food intake (Furuse et al 1999, Ohkubo et al 2002; thus alternative functions other than orexigenic actions should be clarified. To address this issue, we aimed to clone chicken OXR (cOXR) cDNA and reveal tissue distribution of the mRNA in both central and peripheral tissues in the chicken.…”

Section: Atacatcgtcgtgttcatcgtggctctcgtcgggaacgtgctggtttgtattgcagtgtgmentioning

confidence: 99%

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cDNA cloning of chicken orexin receptor and tIssue distribution: sexually dimorphic expression in chicken gonads

Ohkubo¹,

Tsukada²,

Shamoto³

2003

Journal of Molecular Endocrinology

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Orexin-A and -B are known to stimulate food intake in mammals. However, the critical roles of orexins in birds are not fully understood, since orexins have no stimulatory effect on food intake in the chicken. To understand the physiological role(s) of orexins in birds, we have cloned chicken orexin receptor (cOXR) cDNA by RT-PCR, and analysed the tissue distribution of OXR mRNA in the chicken. The cOXR cDNA is 1869 bp long and encodes 501 amino acids. The cloned cDNA for cOXR corresponds to the type 2 OXR in mammals, and shows approximately 80% similarity to those of mammals at the amino acid level. Expression analysis by RNase protection assay revealed OXR mRNA was distributed widely in brain regions, and expression in the cerebrum, hypothalamus and optic tectum were abundant. In peripheral tissues, OXR mRNA was expressed in the pituitary gland, adrenal gland and testis, but no mRNA expression was observed in other tissues examined. Furthermore, we found that the amount of cOXR mRNA was different between testis and ovary, while prepro-orexin mRNA is equally expressed in the gonads of both sexes in the chicken. These data indicate that the orexins have neuroendocrine actions in chickens, which are mediated through hypothalamic receptors as has been observed in mammals. In addition, orexin may have specific role(s) in the regulation of gonadal function in which sex-dependent mechanisms could be involved.

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Section: Atacatcgtcgtgttcatcgtggctctcgtcgggaacgtgctggtttgtattgcagtgtgmentioning

confidence: 99%

Section: Atacatcgtcgtgttcatcgtggctctcgtcgggaacgtgctggtttgtattgcagtgtgmentioning

confidence: 99%

cDNA cloning of chicken orexin receptor and tIssue distribution: sexually dimorphic expression in chicken gonads

Ohkubo¹,

Tsukada²,

Shamoto³

2003

Journal of Molecular Endocrinology

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“…The control group was injected with an equal volume of EB saline solution. The injection method has been shown to cause no stress on chicks: ICV injection of 0.1% EB saline solution has no affect on feeding behavior [29] or corticosterone release [30] when compared with intact chicks without injections. Chicks were exposed to low-temperature (20°C) for 3 h after ICV injection and were sacrificed thereafter.…”

Section: Icv Injectionmentioning

confidence: 99%

Homeothermy in neonatal chicks exposed to low environmental temperature with or without intracerebroventricular administration of corticotropin‐releasing factor

Mujahid

Furuse

2008

FEBS Letters

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To determine the mechanism of sensitivity to lowtemperature exposure (20°C for 3 h) and corticotropin-releasing factor (CRF) induced increased homeothermy, we investigated gene transcripts of putative thermogenic proteins and mitochondrial fatty acid (FA)-oxidation enzymes in neonatal chicks. The hypothalamic-pituitary-adrenal (HPA) axis in low-temperature-exposed neonatal chicks was activated by central administration of CRF. Neonatal chicks showed hypothermia on exposure to low-temperature, with no enhancement of HPA axis and gene transcripts of avian adenine nucleotide translocator, avian uncoupling protein, avian peroxisome-proliferator-activated receptor-c co-activator-1a, and mitochondrial FA transport and oxidation enzymes in vital organs. However, central administration of CRF activated the HPA axis under low environmental temperature and induced increased homeothermy that was associated with the enhancement of gene transcripts and activities of mitochondrial FA-oxidation enzymes in the liver and heart.

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“…There is no injection-induced physiological stress by this technique in neonatal chickens (Saito et al 2005). All injections were done in a volume of 10 μl (Furuse et al 1999). The control group received control solution (saline containing 10 μl Evans) (Furuse et al 1999).…”

Section: Animalsmentioning

confidence: 99%

“…All injections were done in a volume of 10 μl (Furuse et al 1999). The control group received control solution (saline containing 10 μl Evans) (Furuse et al 1999). At the end of the experiments, to recognize accuracy of injection, chicken were killed by decapitation.…”

Section: Animalsmentioning

confidence: 99%

Effect of opioid receptors agonists on feeding behaviour using different diets in ad libitum fed neonatal chicken

Zendehdel¹,

Ebrahimnezhad²,

Ghalehkandi³

et al. 2017

CZECH J ANIM SCI

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Arva S., Zendehdel M., EbrahimNezhad Y., Ghiasi Ghalehkandi J., Hassanpour S., Aghdam Shahryar H. (2017): Effect of opioid receptors agonists on feeding behaviour using different diets in ad libitum fed neonatal chicken. Czech J. Anim. Sci., 62, 98-109.Despite progress in studying the role of opioids in reward, the effect of opioid receptors on feeding behaviour in ad libitum fed meat-type chicken offered different diet types is still unclear. So in this study, 12 experiments (each included 4 groups) were designed to determine the role of μ, δ, and κ receptors with different diets on feeding responses in ad libitum fed neonatal chicken. In Experiment 1, group A chicken were intracerebroventricularly (ICV) injected with saline, groups B-D chicken were ICV injected with DAMGO (µ-opioid receptor agonist; 125, 250, and 500 pmol), then standard diet without fat was offered. In Experiment 2, group A chicken were ICV injected with saline, groups B-D chicken were ICV injected with DAMGO (125, 250, and 500 pmol) and diet with nutrient energy ratio 20% below standard was provided to the birds. Experiments 3-4 were similar to Experiment 1, except after injection, diets containing nutrient energy ratio 20% above standard and standard diet with fat were provided to the birds, respectively. In Experiment 5, chicken were ICV injected with saline, DPDPE (δ-opioid receptor agonist) at doses of 20, 40, and 80 nmol, and then received standard diet without fat. Experiments 6-8 were similar to Experiment 5 in which diet containing nutrient energy ratio by 20% lower than standard, diet containing nutrient energy ratio by 20% higher than standard, and diet containing fat were provided instead of standard diet without fat to the birds, respectively. In Experiment 9, birds received ICV injection of saline and U-50488H (κ-opioid receptor agonist; 10, 20, and 40 nmol) and were provided standard diet without fat. Experiments 10-12 were similar to Experiment 9 but after ICV injection, birds were fed diet containing by 20% lower nutrient energy ratio, diet containing by 20% higher nutrient energy ratio, and standard diet containing fat, respectively. Then the cumulative food intake was measured until 180 min post injection. According to the results, DAMGO decreased while DPDPE and U-50488H increased the food intake (P < 0.05). These findings suggest endogenous governing food preferences via δ-and κ-opioid receptor in ad libitum fed neonatal chicken.

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Intracerebroventricular injection of orexins does not stimulate food intake in neonatal chicks

Cited by 216 publications

References 9 publications

cDNA cloning of chicken orexin receptor and tIssue distribution: sexually dimorphic expression in chicken gonads

cDNA cloning of chicken orexin receptor and tIssue distribution: sexually dimorphic expression in chicken gonads

Homeothermy in neonatal chicks exposed to low environmental temperature with or without intracerebroventricular administration of corticotropin‐releasing factor

Effect of opioid receptors agonists on feeding behaviour using different diets in ad libitum fed neonatal chicken

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