Intracerebroventricular Administration of Ouabain to Rats Changes the Expression of NMDA Receptor Subunits in Cerebral Cortex and Hippocampus

Bersier, María Geraldina; Arnaiz, Georgina Rodrı́guez de Lores

doi:10.1007/s11064-009-9956-1

Cited by 7 publications

(11 citation statements)

References 48 publications

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“…Area-dependent effect had been observed after ouabain injection (1-100 nmol) on assaying the expression of NMDA subunits. Such treatment only produces in cerebral cortex some increase in the expression of NR1 subunit and NR2D subunit whereas in hippocampus it leads mainly to decreases in the expression of NR2A-NR2C NMDA subunits as well as to either increase or decrease of NR1 subunit according to the drug dose employed [20]. These results suggest that endobain E produced similar changes on the expression of NMDA receptor subunits and Na ?…”

Section: Discussionmentioning

confidence: 77%

“…Taken jointly, findings indicated that ouabain and endogenous inhibitor endobain E differentially increased expression of NMDA receptor subunits [19,20] and that this effect was concomitant with changes in Na ? , K ?…”

Section: Discussionmentioning

confidence: 89%

“…-ATPase a3 subunit (present findings). Concomitantly, it was observed an enhancement of NMDA receptor NR1 subunit expression, known to be crucial for this receptor function [20].…”

Section: Discussionmentioning

confidence: 95%

“…-ATPase inhibitors was analyzed. Both qualitative and quantitative alterations of NMDA receptor subunit expression were recorded [19,20].…”

Section: Discussionmentioning

confidence: 99%

“…The administration of endobain E and ouabain led to differential regulation of NMDA subunit expression [19,20]. Taken into account these findings, to learn about a potential interplay between the regulation of NMDA receptor and Na ?…”

Section: Introductionmentioning

confidence: 99%

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Changes in Na+, K+-ATPase Activity and Alpha 3 Subunit Expression in CNS After Administration of Na+, K+-ATPase Inhibitors

2010

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The expression of Na(+), K(+)-ATPase α3 subunit and synaptosomal membrane Na(+), K(+)-ATPase activity were analyzed after administration of ouabain and endobain E, respectively commercial and endogenous Na(+), K(+)-ATPase inhibitors. Wistar rats received intracerebroventricularly ouabain or endobain E dissolved in saline solution or Tris-HCl, respectively or the vehicles (controls). Two days later, animals were decapitated, cerebral cortex and hippocampus removed and crude and synaptosomal membrane fractions were isolated. Western blot analysis showed that Na(+), K(+)-ATPase α3 subunit expression increased roughly 40% after administration of 10 or 100 nmoles ouabain in cerebral cortex but remained unaltered in hippocampus. After administration of 10 μl endobain E (1 μl = 28 mg tissue) Na(+), K(+)-ATPase α3 subunit enhanced 130% in cerebral cortex and 103% in hippocampus. The activity of Na(+), K(+)-ATPase in cortical synaptosomal membranes diminished or increased after administration of ouabain or endobain E, respectively. It is concluded that Na(+), K(+)-ATPase inhibitors modify differentially the expression of Na(+), K(+)-ATPase α3 subunit and enzyme activity, most likely involving compensatory mechanisms.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 89%

“…-ATPase a3 subunit (present findings). Concomitantly, it was observed an enhancement of NMDA receptor NR1 subunit expression, known to be crucial for this receptor function [20].…”

Section: Discussionmentioning

confidence: 95%

“…-ATPase inhibitors was analyzed. Both qualitative and quantitative alterations of NMDA receptor subunit expression were recorded [19,20].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Changes in Na+, K+-ATPase Activity and Alpha 3 Subunit Expression in CNS After Administration of Na+, K+-ATPase Inhibitors

2010

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Influence of N‐methyl‐D‐aspartate receptors on ouabain activation of nuclear factor‐κB in the rat hippocampus

Kawamoto

Lima

Munhoz

et al. 2011

J of Neuroscience Research

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It has been shown that ouabain (OUA) can activate the Na,K-ATPase complex and mediate intracellular signaling in the central nervous system (CNS). Inflammatory stimulus increases glutamatergic transmission, especially at N-methyl-D-aspartate (NMDA) receptors, which are usually coupled to the activation of nitric oxide synthase (NOS). Nuclear factor-κB (NF-κB) activation modulates the expression of genes involved in development, plasticity, and inflammation. The present work investigated the effects of OUA on NF-κB binding activity in rat hippocampus and the influence of this OUA-Na,K-ATPase signaling cascade in NMDA-mediated NF-κB activation. The findings presented here are the first report indicating that intrahippocampal administration of OUA, in a concentration that did not alter Na,K-ATPase or NOS activity, induced an activation of NF-κB, leading to increases in brain-derived neurotrophic factor (Bdnf), inducible NOS (iNos), tumor necrosis factor-α (Tnf-α), and B-cell leukemia/lymphoma 2 (Bcl2) mRNA levels. This response was not linked to any significant signs of neurodegeneration as showed via Fluoro-Jade B and Nissl stain. Intrahippocampal administration of NMDA induced NF-κB activation and increased NOS and α(2/3) -Na,K-ATPase activities. NMDA treatment further increased OUA-induced NF-κB activation, which was partially blocked by MK-801, an antagonist of NMDA receptor. These results suggest that OUA-induced NF-κB activation is at least in part dependent on Na,K-ATPase modulatory action of NMDA receptor in hippocampus. The interaction of these signaling pathways could be associated with biological mechanisms that may underlie the basal homeostatic state linked to the inflammatory signaling cascade in the brain.

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Ouabain increases neuronal branching in hippocampus and improves spatial memory

et al. 2018

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Intracerebroventricular Administration of Ouabain to Rats Changes the Expression of NMDA Receptor Subunits in Cerebral Cortex and Hippocampus

Cited by 7 publications

References 48 publications

Changes in Na+, K+-ATPase Activity and Alpha 3 Subunit Expression in CNS After Administration of Na+, K+-ATPase Inhibitors

Changes in Na+, K+-ATPase Activity and Alpha 3 Subunit Expression in CNS After Administration of Na+, K+-ATPase Inhibitors

Influence of N‐methyl‐D‐aspartate receptors on ouabain activation of nuclear factor‐κB in the rat hippocampus

Ouabain increases neuronal branching in hippocampus and improves spatial memory

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