Intracellular transmission in cell volume regulation in Ehrlich ascites tumor cells

Hoffmann, Else K.

doi:10.1002/(sici)1097-010x(19971201)279:5<398::aid-jez2>3.0.co;2-j

Cited by 6 publications

(7 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In experiments in a variety of cells, including amphibian gall bladder cells (Foskett and Spring, 1985), green crab axons (Gilles et al, 1986), rabbit proximal tubules (Linshaw et al, 1991), cultured PC12 cells (Cornet et al, 1988), and mammalian tumor cells (Cornet at al., 1993), cytochalasin was shown to impact the cell's ability to undergo regulatory volume increase (RVI) and/or decrease (RVD). This relationship appeared particularly strong with regard to the RVD response and it was hypothesized that the actin cytoskeleton may be involved in either limiting the extent of cell swelling, and thus allowing RVD to take place, activating the K ϩ and Clchannels known to be important in RVD, undergoing actomyosin-based cortical contraction to aid in cell shrinkage, or all of the above Hoffman, 1997).…”

Section: Alterations In the Structuralmentioning

confidence: 99%

“…The fact that actin filaments are often concentrated in the submembranous cell cortex region makes them appealing candidates for controlling the shape changes associated with alterations in cell volume. Although the regulation of cell volume is often considered to be controlled exclusively at the level of the plasma membrane via the activity of various membrane transport systems, a number of past reviews have implicated the involvement of the actin cytoskeleton in this crucial cellular process (see Kleinzeller and Ziyah, 1990;Mills et al, 1994;Hoffman, 1997;Cantiello, 1997).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Relationships between the actin cytoskeleton and cell volume regulation

Henson

1999

Microsc. Res. Tech.

View full text Add to dashboard Cite

The actin cytoskeleton mediates a variety of essential biological functions in cells, including division, shape changes, and movement. A number of studies have suggested that the abundant submembranous actin cytoskeleton present in the cortex of many cell types is involved in the regulation of cell volume. This relationship is supported by numerous works which document the changes in the structural organization of the actin cytoskeleton which accompany cell volume changes and the F‐actin‐dependence of the regulatory volume responses. In addition, other studies demonstrate structural and functional relationships between the actin cytoskeleton and the membrane transporters known to be involved in cell volume homeostasis. This review provides a summary of the current level of knowledge in this area and discusses the mechanisms which may underlie the linkage between the actin cytoskeleton and cell volume regulation. Microsc. Res. Tech. 47:155–162, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

Section: Alterations In the Structuralmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Relationships between the actin cytoskeleton and cell volume regulation

Henson

1999

Microsc. Res. Tech.

View full text Add to dashboard Cite

show abstract

“…This acceleration was due to activation of ChTX-sensitive K + channels distinct from and in parallel with the swelling-induced ChTX-insensitive K + channels (see [4]). Previous studies using Ehrlich cells have provided evidence for a role for the 5-lipoxygenase product leukotriene D 4 (LTD 4 ) as a regulator of swelling-activated channels [18], and it has been demonstrated that addition of 3 nM LTD 4 to Ehrlich cells in hypotonic, Ca 2+ -free medium accelerates the RVD response in the absence of any detectable increase in ([Ca 2+ ] i ) [14]. Furthermore, various lipoxygenase products have been found to play a role in the RVD response, and to modulate the activity of K + and Cl -channels in several other cell types (see [5] for references).…”

Section: Introductionmentioning

confidence: 99%

“…LTD 4 [21], bradykinin [10,16], ATP and UTP [23] are all found to evoke a transient increase in [Ca 2+ ] i in Ehrlich cells due to release from intracellular Ca 2+ stores sensitive to inositol 1,4,5-trisphosphate (Ins [1,4,5]P 3 ) as well as a capacitative Ca 2+ influx. In addition they all elicited rapid cell shrinkage, presumably due to activation of Ca 2+ -activated K + and Cl -channels.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Leukotriene D 4 (LTD 4 ) activates charybdotoxin-sensitive and -insensitive K + channels in Ehrlich ascites tumor cells

Hoffmann¹

1999

Pfl�gers Archiv European Journal of Physiology

View full text Add to dashboard Cite

The putative role for ATP, UTP, bradykinin and leukotriene D4 (LTD4) in the activation of the charybdotoxin-insensitive, volume-activated K+ leak pathway has been assessed in Ehrlich cells. K+ channel activity is evaluated from bumetanide-insensitive 86Rb+ efflux using Rb+ as a tracer for K+. Addition of the Ca2+-mobilizing agonists bradykinin, ATP, UTP or LTD4 accelerates the regulatory volume decrease (RVD) response and activates a fast bumetanide-insensitive, charybdotoxin-sensitive efflux of K+. In addition LTD4 activates a charybdotoxin-insensitive K+ efflux, whereas bradykinin, ATP and UTP do not. The charybdotoxin-insensitive K+ efflux dominates after addition of LTD4 at concentrations too low to elicit an increase in [Ca2+]i but still high enough to be effective in accelerating the RVD response. The EC50 values for LTD4-induced K+ effluxes are estimated at 2 nM and 15 nM for the charybdotoxin-insensitive and charybdotoxin-sensitive components, respectively. The LTD4 (cysLT1) receptor antagonist L660,711(MK-571) blocks the activation of the charybdotoxin-sensitive but not the charybdotoxin-insensitive K+ efflux. Thus, LTD4 activates two different K+ leak pathways in Ehrlich cells, one pathway activated by an increase in [Ca2+]i and the other via an alternative signalling pathway. LTD4 is thus a potential candidate for an autocrine messenger activating the Ca2+-independent, charybdotoxin-insensitive K+ channel during the RVD response in Ehrlich cells.

show abstract

Organic osmolyte channels in cell volume regulation in vertebrates

Perlman

Goldstein

1999

J. Exp. Zool.

View full text Add to dashboard Cite

Volume‐activated organic osmolyte channels are found in a variety of vertebrates and cell types and show both common and disparate features. Upon exposure to hypotonic conditions, organic compounds such as taurine are released through these channels, reducing the intracellular solute concentration and thereby restoring cell volume. Various structurally diverse membrane proteins have been proposed as the channel. Accumulating evidence suggests that some of these proteins may play a more significant role as regulators than as the channel itself. Intracellular ionic strength may also modulate the release of organic osmolytes through these channels. J. Exp. Zool. 283:725–733, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

Intracellular transmission in cell volume regulation in Ehrlich ascites tumor cells

Cited by 6 publications

References 74 publications

Relationships between the actin cytoskeleton and cell volume regulation

Relationships between the actin cytoskeleton and cell volume regulation

Leukotriene D 4 (LTD 4 ) activates charybdotoxin-sensitive and -insensitive K + channels in Ehrlich ascites tumor cells

Organic osmolyte channels in cell volume regulation in vertebrates

Contact Info

Product

Resources

About