Intracellular Trafficking Modulation by Ginsenoside Rg3 Inhibits Brucella abortus Uptake and Intracellular Survival within RAW 264.7 Cells

Huy, Tran Xuan Ngoc; Reyes, Alisha Wehdnesday Bernardo; Hop, Huynh Tan; Arayan, Lauren Togonon; Min, Wongi; Lee, Hu Jang; Rhee, Man Hee; Chang, Hong; Kim, Suk

doi:10.4014/jmb.1609.09060

Cited by 11 publications

(10 citation statements)

References 21 publications

(26 reference statements)

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“…To counteract aging, actin polymerization in cells can be inhibited by ginseng. The F-actin content of RAW 264.7 cells was decreased by treatment with the ginsenoside Rg3, a saponin extracted from ginseng [31] . Moreover, the F-actin:G-actin ratio was decreased in leptin-treated cardiomyocytes compared with the controls, indicating an increase in the quantity of F-actin [32] .…”

Section: Discussionmentioning

confidence: 99%

Effects of red ginseng on the elastic properties of human skin

Park

Han

Moon

et al. 2020

Journal of Ginseng Research

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Background Red ginseng contains components, including microelements, vitamins, essential oils, and fatty acids, that can be used in skincare to delay the aging process. We investigated the effects of red ginseng treatment on skin elasticity by assessing cellular stiffness and measuring collagen protein synthesis. Methods Human dermal fibroblasts were treated with red ginseng, and the resulting changes in stiffness were investigated using atomic force microscopy. Cytoskeletal changes and mRNA expression of biomarkers of aging, including that of procollagens I and VII, elastin, and fibrillin-1, were investigated. Collagen in a human skin equivalent treated with red ginseng was visualized via hematoxylin and eosin staining, scanning electron microscopy, and atomic force microscopy. Results and conclusion The stiffness of fibroblasts was significantly reduced by treatment with red ginseng concentrations of ≥ 0.8 mg/mL. The ratio of F-actin to G-actin decreased after treatment, which corresponded to a change in fibroblast stiffness. The storage modulus ( G ′) and loss modulus ( G ″) of the skin equivalent were both lowered by red ginseng treatment. This result indicates that the viscoelasticity of the skin equivalent can be restored by red ginseng treatment.

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Section: Discussionmentioning

confidence: 99%

Effects of red ginseng on the elastic properties of human skin

Park

Han

Moon

et al. 2020

Journal of Ginseng Research

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“…The immunoblotting analysis was performed as previously described [ 53 ]. Cells were subcultured in a 6-well plate and incubated overnight.…”

Section: Methodsmentioning

confidence: 99%

Cobalt (II) Chloride Regulates the Invasion and Survival of Brucella abortus 544 in RAW 264.7 Cells and B6 Mice

et al. 2022

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The effects of Cobalt (II) chloride (CoCl2) in the context of Brucella abortus (B. abortus) infection have not been evaluated so far. Firstly, we found that CoCl2 treatment inhibited the phagocytosis of B. abortus into RAW 264.7 cells. The inhibition of bacterial invasion was regulated by F-actin formation and associated with a reduction in the phosphorylation of ERK1/2 and HIF-1α expression. Secondly, the activation of trafficking regulators LAMP1, LAMP2, and lysosomal enzyme GLA at the transcriptional level activated immune responses, weakening the B. abortus growth at 4 h post-infection (pi). The silencing of HIF-1α increased bacterial survival at 24 h pi. At the same time, CoCl2 treatment showed a significant increase in the transcripts of lysosomal enzyme HEXB and cytokine TNF-α and an attenuation of the bacterial survival. Moreover, the enhancement at the protein level of HIF-1α was induced in the CoCl2 treatment at both 4 and 24 h pi. Finally, our results demonstrated that CoCl2 administration induced the production of serum cytokines IFN-γ and IL-6, which is accompanied by dampened Brucella proliferation in the spleen and liver of treated mice, and reduced the splenomegaly and hepatomegaly. Altogether, CoCl2 treatment contributed to host resistance against B. abortus infection with immunomodulatory effects.

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“…RGSF-A influences also intracellular trafficking of B. abor tus and favors interaction of B. abortus-containing phagosomes (BCPs) with LAMP-1. LAMP-1 is transmembrane protein, that is responsible for the fusion of lysosomes with phagosomes, enabling the connection of BCPs with lysosome and elimination of bacteria (Arayan et al, 2015;Reyes et al, 2016;Huy et al, 2017). Huy et al (2017) have proven that ginsenoside Rg3 -panaxadiol saponin components of RGSF-A have been the major factor controlling brucellosis.…”

Section: Treatment Of Brucellosismentioning

confidence: 99%

Brucella – Virulence Factors, Pathogenesis and Treatment

Głowacka

Żakowska

Naylor

et al. 2018

Polish Journal of Microbiology

133

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Brucellae are Gram-negative, small rods infecting mammals and capable of causing disease called brucellosis. The infection results in abortion and sterility in domestic animals (sheeps, pigs, rams etc). Especially dangerous for humans are: Brucella melitensis, Brucella suis, Brucella abortus, and Brucella canis that trigger unspecific symptoms (flu-like manifestation). Brucella rods are introduced via host cells, by inhalation, skin abrasions, ingestion or mucosal membranes. The most important feature of Brucella is the ability to survive and multiply within both phagocytic and non-phagocytic cells. Brucella does not produce classical virulence factors: exotoxin, cytolisins, exoenzymes, plasmids, fimbria, and drug resistant forms. Major virulence factors are: lipopolysaccharide (LPS), T4SS secretion system and BvrR/BvrS system, which allow interaction with host cell surface, formation of an early, late BCV (Brucella Containing Vacuole) and interaction with endoplasmic reticulum (ER) when the bacteria multiply. The treatment of brucellosis is based on two-drug therapy, the most common combinations of antibiotics are: doxycycline with rifampicin or fluoroquinolones with rifampicin. Currently, also other methods are used to disrupt Brucella intracellular replication (tauroursodeoxycholic acid or ginseng saponin fraction A).

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Intracellular Trafficking Modulation by Ginsenoside Rg3 Inhibits Brucella abortus Uptake and Intracellular Survival within RAW 264.7 Cells

Cited by 11 publications

References 21 publications

Effects of red ginseng on the elastic properties of human skin

Effects of red ginseng on the elastic properties of human skin

Cobalt (II) Chloride Regulates the Invasion and Survival of Brucella abortus 544 in RAW 264.7 Cells and B6 Mice

Brucella – Virulence Factors, Pathogenesis and Treatment

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